Comparison of Enzyme Production by Halophilic Bacteria in Sterilized and Non-Sterilized Conditions

Abstract

In present study, we aimed to determine the enzymes of moderately halophilic bacteria isolated from Serefl ikochisar Lake the largest salt lake in Turkey and compared their enzymes in sterilized and non-sterilized conditions to obtain any contamination from non-sterilized growth condition. The enzymes of moderately halophilic bacteria isolated from Şerefl ikoçhisar Lake were determined by SDS-PAGE. In addition of zymogram analysis, the enzymes from the moderately halophilic bacteria cultured in sterilized and non-sterilized growth condition were compared on SDS-PAGE to determine any contamination in non-sterilized growth condition. The enzyme bands in both conditions were similar and therefore the investigation suggests that the moderately halophilic isolates can be easily cultured in non-sterilized growth conditions without sterilization of growth medium and thereby energy cost can be reduce to produce such enzymes for industrial applications

Keywords

• Halophilic bacteria, enzyme production, zymogram analysis