In vitro Regeneration of Vanilla (Vanilla planifolia L.)

Abstract

Vanilla is a high demanding product sold at high prices in world markets. Natural vanilla has high economic value and is theworld’s second most expensive spices after saffron. Despite its economic value, it has been one of the least studied species of vanilla plants among other orchid species. Vanilla’s synthetic form (vanillin) can be obtained from various chemical methods by using starting materials (coniferin, guaiacol, eugenol and lignin), but the vanilla flavor can naturally be obtained only from vanilla fruit. Micropropagation is an alternative technique for economically important plant production more effectively used with minimum starting material. For this reason, in the study, regeneration of the vanilla plant in vitro were carried out. Seed, young leaves, node and  maerial parts of V. planifolia L. were used as an initial material. The highest callus regeneration (80%) from node explants was obtained in VK4 (MS+1 mg/L 2,4-D + 1 mg/L BAP) nutrient medium, while 100% direct shoot regeneration was provided in VK3 (MS+1 mg/L 2,4-D + 0,5 mg/L BAP) and VK6 (MS+1 mg/L BAP + 0,5 mg/L NAA) nutrient media. In addition, 50% rooting percentage was obtained in VK6 medium. Indirect shoot regeneration ratio was determined as 33,3% in VK2 (MS + 1 mg / L 2,4-D + 0,5 mg / L KIN) and 71,4% of indirect embryo formation was detected in VK4 medium. All embryos stayed in globules stages

Keywords

• vanilla,Vanilla planifolia L.,regeneration,in vitro

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