In Vitro Establishment Procedures of Dog Rose (Rosa canina)

Abstract

Dog rose (Rosa canina) is one of the medicinal plants with important role on vitamin C and active ingredients but a few researches has been conducted on the in vitro efficient establishment of this plant. Therefore, for optimization of establishment stage of R. canina, this study was carried out by 8 decontamination procedures included T1: 5% Sodium Hypochlorite, T2: 10% Povidone Iodine, T3: commercial Dettol, T4: commercial Banzalkonium Chloride, T5: Mercury (II) Chloride for 5 min, T6: Mercury (II) Chloride for 8 min, T7: 2.5% Sodium Hypochlorite with Cefotaxime and Tetracycline, T8: Mercury (II) Chloride for 2 min with Cefotaxime and Tetracycline. for elimination of bacteria and fungi contamination. In this study minimum browning percentage was observed in T7 and T8 (6 and 8% respectively), Bacterial contamination was completely controlled in T7 and T8, Fungi contamination percentage was completely controlled (100%) by T2, T3, T4, T6 and partially controlled by T8 and non-controlled by T7. Maximum bud break percentage was observed in T8, T7 and T2. The highest shoot length in T2 and T7 but the lowest shoot lengths were observed in T6. In T2, T3 and T7, node numbers were more than other decontamination treatments. T8 on the base of browning percentage, bacteria and fungi contamination percentage, bud break percentage was better than other procedures. T7 and T2 on the base of shoot length and node numbers were decontamination procedure better than other procedures. In this investigation decontamination procedures are considered as one the most important steps for successful implementation of biotechnological techniques for R. canina

Keywords

• Decontamination procedures bacterial contamination,fungi contamination,In vitro,Rosa canina

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