In vitro high frequency axillary shoot regeneration of Roundleaf toothcup-Rotala rotundifolia [(BuchHam. ex Roxb) Koehne]

Abstract

Roundleaf toothcup-Rotala rotundifolia [(BuchHam. ex Roxb) Koehne] is an amphibious aquatic plant of Southeast Asia used as medicinal and ornamental purpose. The shoot tip explant of R. rotundifolia was aseptically excised and cultured on MS (Murashige and Skoog) medium enriched with 0.05-1.60 mg/l Thidiazuron (TDZ) that resulted in callus induction without any shoot induction after 8 weeks of inoculation. Thereafter, the explants were transferred to MSO for further 4 weeks that resulted in 100% shoot regeneration frequency, 29.33-59.67 shoots per explant alongwith mean shoot length ranged 0.20-1.87 cm. Maximum number of 59.67 shoot alongwith mean longer shoots (1.87 cm) were achieved on MS medium with 0.05 mg/l TDZ. Further increase of TDZ concentration in the culture medium inhibited the both shoots per explant and shoot length. Soots with ≈1.0 cm length were successfully rooted on medium enriched with 0.25 mg/l Indole-3-butyric Acid (IBA) that was followed by successfıul adaptation of plantlets in the aquariums filled with tap water. The protocol developed can be employed for future biotechnological studies like genetic transformation. 

Keywords

• Axillary shoots,In vitro,Shoot tip,Thidiazuron

References

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