Multiplication Of Seed Mini Tubers From In Vitro Potato Plantlets

Abstract

The promising clones developed in the last stage of potato breeding should be produced with the meristem culture required for the registration experiments. Production is required to obtain a small number of seeds from the in vitro plantlets produced by the meristem culture. This investigation carried out two different locations (Nigde-Turkey and Tokat-Turkey) in 2016. The aim of this study is to determine in which medium the tubers from the in vitro plantlets can be determined faster and to obtain seeds with suitable characteristics. In this study, 16 different promising clones were used. Plants from in vitro plantles of these clones were compared in pot and soil conditions under net greenhouse conditions. A mixture of 2/3 peat and 1/3 perlite was used as a medium in the pots.

According to the findings, the number of tubers per pot and the number of tubers per plant was higher in pots condition in the net greenhouse than in the field condition in the net greenhouses whereas in the field condition in the net greenhouses, the number of tubers per plant and the number of tubers were less than, although the number of plants planted per unit area was higher. Therefore, it has been determined that more space is required for the amount of seeds required for location experiments.

While the average number of tubers per plant from the tubers planted directly on the soil in the net greenhouses ranged from 2.4 to 5.4, the number in the net greenhouse field condition ranged from 5.7 to 14.1. Similarly, while tuber yield per plant in the field condition ranged from 123.1 to 383.6 g, in the peat-pearlite pot medium ranged from 290.3 to 470.9 g. It was also determined that the tuber seeds produced in pots and peat-perlite medium are more homogenous and healthier. As a result of the study, it was determined that the production of the tubers from the in vitro plantlets in peat-pearlite pots in the net-greenhouse condition is better results than other condition in net greenhouse. It has been determined that more area is required for direct field planting the net greenhouse to produce the same amount of seed nodules.

Keywords

• potato,mini tuber,multiplication of mini tuber,net greenhouse

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