Biodegradation of Diesel by Acinetobacter lwoffii and Vibrio alginolyticus Isolated from Ship Dismantling Facility in Tanjungjati Coast, Madura, Indonesia

Abstract

Presence of diesel in environment will cause serious effects to ecosystembecause the components of diesel are potentially carcinogenic and toxic for environment. Diesel contaminated seawater and soil samples were collected from ship dismantling facility in Tanjungjati coast, Madura, Indonesia.Isolation of diesel degrading bacteria was performed using serial dilution method.Preliminary and secondary screening of diesel degrading bacteria were carried out by using nutrient agar medium and Mineral Salt Medium (MSM) containing diesel (0; 5; 10; and 15% (v/v))as a carbon source.The diesel degradation was conducted by inoculating bacteria that potentially degrade diesel into MSM with optimum concentration and diesel degradation percentage was detected by GC-MS analysis.In this study, thirteen bacteria strains were isolated which had the potential to degrade diesel. Based on preliminary and secondary screening, the optimum concentration was found to be 10% (v/v) of diesel and bacteria coded AT and EL have highest growth with 8.01 and 8.13 log CFU/mL for 7 days incubation. Bacterial code of AT and EL were identified as Acinetobacter lwoffii and Vibrio alginolyticus. The result showed that V. alginolyticus and A. lwoffii were able to degrade diesel about 26.78% and 1.85% for 14 days of incubation at initial diesel of 10% (v/v). In conclusion, V. alginolyticus may has a potential as an degrader for the bioremediation of marine environments polluted by dieselwith further investigation through biostimulation and bioaugmentation technique.

Keywords

• Acinetobacter lwoffii,Biodegradation,Diesel,Ship dismantling facility,Vibrio alginolyticus

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