Antibiotics resistant Escherichia coli from hospital liquid waste

Abstract

The emergence of antimicrobial-resistant bacteria is a major challenges in hospital waste water disposal. The present study aimed to investigate the presence of antibiotics and chlorine resistant E.coli in hospital liquid waste in Bogor, Indonesia. The samples were obtained from influent, chlorination tank and efluent points of waste treatment installation. The steps of the research were enumeration of bacterial population in Nutrient Agar (NA)and Eosin Methylen Blue Agar (EMBA) medium, hemolytic assay test, biochemical tests, antimicrobials test and diversities of 16S rRNA sequences analysis. The result showed the highest number of total bacterial population were at chlorination point 2.31x 105cfu/ml, followed by influent 1.78 x 105 cfu/ml, and efluent 1.9 x103cfu/ml. Due to hemolytic test for 30 isolates, there were 2 isolates showed beta-hemolytic sign, 4 isolates with alfa-hemolytic sign and 24 isolates showed gamma-hemolytic sign. There were a diversity resistance pattern to antimicrobial agents of E. coli isolates. The 16S rRNA analysis result showed that all of four tested isolates genetically closed to E.coli. The results of the present study gives an evidence aboutemergence possibility of multiantimicrobials resistant bacteria from insufficient disinfection method in hospital liquid waste.

Keywords

• Escherichia coli,antibiotics resistant,hospital liquid waste

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