No Association between Foxp3 Promoter Region Polymorphisms (rs3761548 and rs2232365) and Crimean Congo Hemorrhagic Fever Disease

Abstract

Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne, viral disease that causes severe outbreaks in people throughout large geographical areas. The common feature of viral hemorrhagic fever (VHF) cases is the attack on the cells that initiate the antiviral response and the impaired immune response of the host. There is no study of the variation of the promoter region of Foxp3 gene, which has been shown to be involved in inflammation in studies of autoimmune diseases, in relation to the risk or susceptibility of disease in CCHF patients. Therefore, the aim of our study was to investigate Foxp3 rs3761548 and rs2232365 gene polymorphisms in patients with CCHF. Blood samples taken from 80 CCHF patients and 80 healthy subjects were used in the study. Genotyping was performed by PCR-RFLP followed by total DNA isolation. No statistically significant relationship between Foxp3 gene polymorphisms rs2232365 and rs3761548 and CCHF was found.Although the pathophysiology of CCHF is still not fully understood, there are some mechanisms shown to be related to the immune system. Studies examining other variants in the Foxp3 gene promoter region and of exon and intron mutations may yield beneficial results in terms of the association between gene and disease progression.

Keywords

• Crimean-Congo hemorrhagic fever,Foxp3,polymorphism

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