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Subcellular Localization of Bm4, the Protein Product of ORF4 of Bombyx Mori Nucleopolyhedrovirus

Year 2008, Volume: 2 Issue: 2, 69 - 72, 01.07.2008

Abstract

Bm4, the protein product of open reading frame 4 (Bm4) of Bombyx mori nucleopolyhedrovirus (BmNPV), is a protein whose function is completely unknown. To unravel its biological function, its subcellular localization is a fi rst crucial step. In order to study the subcellular localization of Bm4 in the context of BmNPV infection, we used a recently developed BmNPV bacmid, which is infectious to Bm cell lines, to express Bm4-EGFP fusion protein. With EGFP fused to the 3’ terminus of Bm4 as the reporter gene and by using a confocal scanning microscope, we found that the green fl uorescent signal was localized primarily in the nucleus of Bm cells, however, a weaker signal was also observed in the membrane. Furthermore, bioinformatic analysis showed that there was no nuclear localization signal (NLS) or transmembrane helices (TMHs) in the amino acid sequence of Bm4, indicating that Bm4-EGFP fusion protein may be colocalized with host- or virus-encoded factors in the nucleus, and that Bm4 may have the ability to bind to certain protein located in the membrane. The western blot analysis using the antibody against EGFP detected only a specifi c band of about 67 kDa, which is consistent with the theoretical size of Bm4-EGFP fusion protein, confi rming that EGFP was correctly fused to Bm4. The localization of Bm4-EGFP fusion protein in the nucleus and membrane implies that Bm4 may have two different functions

Year 2008, Volume: 2 Issue: 2, 69 - 72, 01.07.2008

Abstract

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Details

Other ID JA79ZV73PU
Journal Section Research Article
Authors

Wu-jie Su This is me

Yan Wu This is me

Bing Li This is me

Wei-de Shen This is me

Guang Gao This is me

Wen-bing Wang This is me

Publication Date July 1, 2008
Published in Issue Year 2008 Volume: 2 Issue: 2

Cite

APA Su, W.-j., Wu, Y., Li, B., Shen, W.-d., et al. (2008). Subcellular Localization of Bm4, the Protein Product of ORF4 of Bombyx Mori Nucleopolyhedrovirus. Journal of Applied Biological Sciences, 2(2), 69-72.
AMA Su Wj, Wu Y, Li B, Shen Wd, Gao G, Wang Wb. Subcellular Localization of Bm4, the Protein Product of ORF4 of Bombyx Mori Nucleopolyhedrovirus. J.appl.biol.sci. July 2008;2(2):69-72.
Chicago Su, Wu-jie, Yan Wu, Bing Li, Wei-de Shen, Guang Gao, and Wen-bing Wang. “Subcellular Localization of Bm4, the Protein Product of ORF4 of Bombyx Mori Nucleopolyhedrovirus”. Journal of Applied Biological Sciences 2, no. 2 (July 2008): 69-72.
EndNote Su W-j, Wu Y, Li B, Shen W-d, Gao G, Wang W-b (July 1, 2008) Subcellular Localization of Bm4, the Protein Product of ORF4 of Bombyx Mori Nucleopolyhedrovirus. Journal of Applied Biological Sciences 2 2 69–72.
IEEE W.-j. Su, Y. Wu, B. Li, W.-d. Shen, G. Gao, and W.-b. Wang, “Subcellular Localization of Bm4, the Protein Product of ORF4 of Bombyx Mori Nucleopolyhedrovirus”, J.appl.biol.sci., vol. 2, no. 2, pp. 69–72, 2008.
ISNAD Su, Wu-jie et al. “Subcellular Localization of Bm4, the Protein Product of ORF4 of Bombyx Mori Nucleopolyhedrovirus”. Journal of Applied Biological Sciences 2/2 (July 2008), 69-72.
JAMA Su W-j, Wu Y, Li B, Shen W-d, Gao G, Wang W-b. Subcellular Localization of Bm4, the Protein Product of ORF4 of Bombyx Mori Nucleopolyhedrovirus. J.appl.biol.sci. 2008;2:69–72.
MLA Su, Wu-jie et al. “Subcellular Localization of Bm4, the Protein Product of ORF4 of Bombyx Mori Nucleopolyhedrovirus”. Journal of Applied Biological Sciences, vol. 2, no. 2, 2008, pp. 69-72.
Vancouver Su W-j, Wu Y, Li B, Shen W-d, Gao G, Wang W-b. Subcellular Localization of Bm4, the Protein Product of ORF4 of Bombyx Mori Nucleopolyhedrovirus. J.appl.biol.sci. 2008;2(2):69-72.