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Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli

Year 2008, Volume: 2 Issue: 2, 19 - 22, 01.07.2008

Murat Kasap Sinem Torol Gulcin Gacar

Abstract

During construction of genomic libraries from various bacteria, we experienced considerably different transformation effi ciencies among the strains of electrocompetent Escherichia coli cells and investigated the effect of type strain on effi ciency of transformation. Among the fi ve strains tested, Escherichia coli DH10B and TOP10 were found to be the most suitable strains for construction of genomic libraries in our laboratory. This study highlighted the fact that although these commercially available Escherichia coli strains are well characterized, they tend to differentiate and loose their transformation abilities. This may be due to the fact that the number of passages they have gone through affect their membrane structures and permeability. Therefore, every laboratory should test their strains for competency before they use them in their cloning studies

Keywords

Escherichia coli transformation electrocompetency

Year 2008, Volume: 2 Issue: 2, 19 - 22, 01.07.2008

Murat Kasap Sinem Torol Gulcin Gacar

Abstract

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Details

Other ID JA72YC82RC
Journal Section Research Article
Authors

Murat Kasap This is me

Sinem Torol This is me

Gulcin Gacar This is me

Publication Date July 1, 2008
Published in Issue Year 2008 Volume: 2 Issue: 2

Cite

APA Kasap, M., Torol, S., & Gacar, G. (2008). Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. Journal of Applied Biological Sciences, 2(2), 19-22.
AMA Kasap M, Torol S, Gacar G. Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. J.appl.biol.sci. July 2008;2(2):19-22.
Chicago Kasap, Murat, Sinem Torol, and Gulcin Gacar. “Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia Coli”. Journal of Applied Biological Sciences 2, no. 2 (July 2008): 19-22.
EndNote Kasap M, Torol S, Gacar G (July 1, 2008) Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. Journal of Applied Biological Sciences 2 2 19–22.
IEEE M. Kasap, S. Torol, and G. Gacar, “Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli”, J.appl.biol.sci., vol. 2, no. 2, pp. 19–22, 2008.
ISNAD Kasap, Murat et al. “Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia Coli”. Journal of Applied Biological Sciences 2/2 (July 2008), 19-22.
JAMA Kasap M, Torol S, Gacar G. Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. J.appl.biol.sci. 2008;2:19–22.
MLA Kasap, Murat et al. “Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia Coli”. Journal of Applied Biological Sciences, vol. 2, no. 2, 2008, pp. 19-22.
Vancouver Kasap M, Torol S, Gacar G. Comparison of Electrocompetencies of Some Commonly Used Laboratory Strains of Escherichia coli. J.appl.biol.sci. 2008;2(2):19-22.