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Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods

Year 2009, Volume: 3 Issue: 2, 1 - 5, 01.07.2009

Musa Yavuz

Abstract

In situ ruminal digestion procedure is known procedure for estimating ruminal digestion. In situ procedure requires correction for microbial contaminations, and the purine method has been used to correct microbial attachments. The neutral detergent fiber (NDF) method has been proposed as an alternative to the purine method. Objectives of this experiment were to compare purine and NDF method for microbial correction. Alfalfa hay, bermudagrass hay, tall fescue hay, corn and soybean meal samples were selected. Chemical analyses of dry matter (DM), crude protein (CP), NDF, Acid detergent fiber (ADF), NDF-protein (NDIP), ADF-protein (ADIP), lignin, crude oil and ash were determined on selected samples. In the experiment, samples were inserted into the rumen of a cannulated cow for 6, 12, 24, 72, and 96 h to determine in situ digestion. The average microbial purine to nitrogen ratio was determined to be 11.43. In situ digested tall fescue microbial protein corrected CP values with purine and NDF methods were similar within all hours, except 96 h. Other In situ digested sample purine procedure had some interference during sample reading and standard errors were not acceptable level. There were not enough data to compare both methods for other samples

Keywords

In situ ruminal digestion bacterial protein purine neutral detergent fiber

Year 2009, Volume: 3 Issue: 2, 1 - 5, 01.07.2009

Musa Yavuz

Abstract

There are 0 citations in total.

Details

Other ID JA77HP37AJ
Journal Section Research Article
Authors

Musa Yavuz This is me

Publication Date July 1, 2009
Published in Issue Year 2009 Volume: 3 Issue: 2

Cite

APA Yavuz, M. (2009). Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods. Journal of Applied Biological Sciences, 3(2), 1-5.
AMA Yavuz M. Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods. J.appl.biol.sci. July 2009;3(2):1-5.
Chicago Yavuz, Musa. “Bacterial Protein Correction on In Situ Digested Samples With Purine or NDF Methods”. Journal of Applied Biological Sciences 3, no. 2 (July 2009): 1-5.
EndNote Yavuz M (July 1, 2009) Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods. Journal of Applied Biological Sciences 3 2 1–5.
IEEE M. Yavuz, “Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods”, J.appl.biol.sci., vol. 3, no. 2, pp. 1–5, 2009.
ISNAD Yavuz, Musa. “Bacterial Protein Correction on In Situ Digested Samples With Purine or NDF Methods”. Journal of Applied Biological Sciences 3/2 (July 2009), 1-5.
JAMA Yavuz M. Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods. J.appl.biol.sci. 2009;3:1–5.
MLA Yavuz, Musa. “Bacterial Protein Correction on In Situ Digested Samples With Purine or NDF Methods”. Journal of Applied Biological Sciences, vol. 3, no. 2, 2009, pp. 1-5.
Vancouver Yavuz M. Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods. J.appl.biol.sci. 2009;3(2):1-5.