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Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli

Year 2009, Volume: 3 Issue: 3, 55 - 59, 01.11.2009

- Sreevıdhya Lawrance Anbu Rajan Yeragudipati Rajalakshmı Vetrivel Umashankar

Abstract

Trehalose, a non-reducing disaccharide is a major compatible solute, maintains fluidity of membranes and protects the biological structure of the organisms under stress. In this study, trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) genes encoding for trehalose biosynthesis were PCR amplified from genomic DNA of Escherichia coli isolated from Rohu (Labeo rohita) collected from local fish market of Cochin, Kerala, India. The amplified genes were cloned and nucleotide sequences were determined. The sequencing results showed that (TPS) and (TPP) genes contain1,424 bp and 801 bp long ORF encoding 474 and 266 amino acids respectively. In silico sequence analysis revealed that the (TPS) and (TPP) sequences of E. coli were conserved in many eubacteria

Keywords

trehalose-6-phosphate synthase trehalose-6-phosphate phosphatase compatible solutes salt stress

Year 2009, Volume: 3 Issue: 3, 55 - 59, 01.11.2009

- Sreevıdhya Lawrance Anbu Rajan Yeragudipati Rajalakshmı Vetrivel Umashankar

Abstract

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Details

Other ID JA92YG29DE
Journal Section Research Article
Authors

- Sreevıdhya This is me

Lawrance Anbu Rajan This is me

Yeragudipati Rajalakshmı This is me

Vetrivel Umashankar This is me

Publication Date November 1, 2009
Published in Issue Year 2009 Volume: 3 Issue: 3

Cite

APA Sreevıdhya, .-., Rajan, L. A., Rajalakshmı, Y., Umashankar, V. (2009). Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. Journal of Applied Biological Sciences, 3(3), 55-59.
AMA Sreevıdhya, Rajan LA, Rajalakshmı Y, Umashankar V. Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. J.appl.biol.sci. November 2009;3(3):55-59.
Chicago Sreevıdhya, -, Lawrance Anbu Rajan, Yeragudipati Rajalakshmı, and Vetrivel Umashankar. “Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia Coli”. Journal of Applied Biological Sciences 3, no. 3 (November 2009): 55-59.
EndNote Sreevıdhya -, Rajan LA, Rajalakshmı Y, Umashankar V (November 1, 2009) Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. Journal of Applied Biological Sciences 3 3 55–59.
IEEE .-. Sreevıdhya, L. A. Rajan, Y. Rajalakshmı, and V. Umashankar, “Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli”, J.appl.biol.sci., vol. 3, no. 3, pp. 55–59, 2009.
ISNAD Sreevıdhya, - et al. “Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia Coli”. Journal of Applied Biological Sciences 3/3 (November 2009), 55-59.
JAMA Sreevıdhya -, Rajan LA, Rajalakshmı Y, Umashankar V. Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. J.appl.biol.sci. 2009;3:55–59.
MLA Sreevıdhya, - et al. “Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia Coli”. Journal of Applied Biological Sciences, vol. 3, no. 3, 2009, pp. 55-59.
Vancouver Sreevıdhya -, Rajan LA, Rajalakshmı Y, Umashankar V. Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli. J.appl.biol.sci. 2009;3(3):55-9.