Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation

Özge Karakaş; Cüneyt Uçarlı; Nermin Gözükırmızı; Filiz Gürel

Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation

Year 2011, Volume: 5 Issue: 1, 55 - 58, 01.02.2011

Özge Karakaş Cüneyt Uçarlı Nermin Gözükırmızı Filiz Gürel

Abstract

This study describes a protocol for Agrobacterium-mediated transformation of barley mature embryos. Cultured embryos were co-cultivated by EHA 105 strain carrying pCambia2301 binary vector and assayed for transient GUS expression. After 3 days of transformation, GUS expressing spots have been observed over 20.3% of total embryos. Among the tested several pre-treatments, infiltration in a vacuum chamber (400 mm Hg for 20 min) and infection by AGL-1 strain instead of EHA 105 increased the numbers of embryos with GUS staining significantly (p<0.05). PCR and Southern analyses showed that transformation occured in some of the T0 plants which were also tolerant to selective conditions

Keywords

Hordeum vulgare L., Agrobacterium-mediated transformation, mature embryo

Year 2011, Volume: 5 Issue: 1, 55 - 58, 01.02.2011

Özge Karakaş Cüneyt Uçarlı Nermin Gözükırmızı Filiz Gürel

Abstract

There are 0 citations in total.

Details

Other ID	JA67VK79MR
Journal Section	Research Article
Authors	Özge Karakaş This is me Cüneyt Uçarlı This is me Nermin Gözükırmızı This is me Filiz Gürel This is me
Publication Date	February 1, 2011
Published in Issue	Year 2011 Volume: 5 Issue: 1

Cite

APA	Karakaş, Ö., Uçarlı, C., Gözükırmızı, N., Gürel, F. (2011). Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. Journal of Applied Biological Sciences, 5(1), 55-58.
AMA	Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F. Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. J.appl.biol.sci. February 2011;5(1):55-58.
Chicago	Karakaş, Özge, Cüneyt Uçarlı, Nermin Gözükırmızı, and Filiz Gürel. “Assessment of Barley (Hordeum Vulgare L.) Mature Embryos for Agrobacterium-Mediated Transformation”. Journal of Applied Biological Sciences 5, no. 1 (February 2011): 55-58.
EndNote	Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F (February 1, 2011) Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. Journal of Applied Biological Sciences 5 1 55–58.
IEEE	Ö. Karakaş, C. Uçarlı, N. Gözükırmızı, and F. Gürel, “Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation”, J.appl.biol.sci., vol. 5, no. 1, pp. 55–58, 2011.
ISNAD	Karakaş, Özge et al. “Assessment of Barley (Hordeum Vulgare L.) Mature Embryos for Agrobacterium-Mediated Transformation”. Journal of Applied Biological Sciences 5/1 (February 2011), 55-58.
JAMA	Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F. Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. J.appl.biol.sci. 2011;5:55–58.
MLA	Karakaş, Özge et al. “Assessment of Barley (Hordeum Vulgare L.) Mature Embryos for Agrobacterium-Mediated Transformation”. Journal of Applied Biological Sciences, vol. 5, no. 1, 2011, pp. 55-58.
Vancouver	Karakaş Ö, Uçarlı C, Gözükırmızı N, Gürel F. Assessment of barley (Hordeum vulgare L.) mature embryos for Agrobacterium-mediated transformation. J.appl.biol.sci. 2011;5(1):55-8.