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Study of Progesterone interaction with Human Serum Albumin: Spectroscopic approach

Year 2011, Volume: 5 Issue: 1, 35 - 47, 01.02.2011

Abstract

The interaction between progesterone and human serum albumin has been investigated. This interaction was studied by UV-absorption and fluorescence spectroscopy. From spectral analysis progesterone showed a strong ability to quench the intrinsic fluorescence of HSA through a static quenching procedure. The binding constant (K) is estimated 6.56×102 M-1 at 293 K. FT-IR spectroscopy with Fourier self-deconvolution technique was used to determine HSA secondary structure and progesterone binding mechanisms. The observed spectral changes indicate the formation of H-bonding between progesterone and HSA molecules which can be related to the intensity decrease in the absorption band of α-helix relative to that of β-sheets

Year 2011, Volume: 5 Issue: 1, 35 - 47, 01.02.2011

Abstract

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Details

Other ID JA42GK52MU
Journal Section Research Article
Authors

M. M. Abu Teır This is me

J. H. Ghıthan This is me

S. M. Darwısh This is me

M. M. Abu-hadıd This is me

Publication Date February 1, 2011
Published in Issue Year 2011 Volume: 5 Issue: 1

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APA Teır, M. M. A., Ghıthan, J. H., Darwısh, S. M., Abu-hadıd, M. M. (2011). Study of Progesterone interaction with Human Serum Albumin: Spectroscopic approach. Journal of Applied Biological Sciences, 5(1), 35-47.
AMA Teır MMA, Ghıthan JH, Darwısh SM, Abu-hadıd MM. Study of Progesterone interaction with Human Serum Albumin: Spectroscopic approach. J.appl.biol.sci. February 2011;5(1):35-47.
Chicago Teır, M. M. Abu, J. H. Ghıthan, S. M. Darwısh, and M. M. Abu-hadıd. “Study of Progesterone Interaction With Human Serum Albumin: Spectroscopic Approach”. Journal of Applied Biological Sciences 5, no. 1 (February 2011): 35-47.
EndNote Teır MMA, Ghıthan JH, Darwısh SM, Abu-hadıd MM (February 1, 2011) Study of Progesterone interaction with Human Serum Albumin: Spectroscopic approach. Journal of Applied Biological Sciences 5 1 35–47.
IEEE M. M. A. Teır, J. H. Ghıthan, S. M. Darwısh, and M. M. Abu-hadıd, “Study of Progesterone interaction with Human Serum Albumin: Spectroscopic approach”, J.appl.biol.sci., vol. 5, no. 1, pp. 35–47, 2011.
ISNAD Teır, M. M. Abu et al. “Study of Progesterone Interaction With Human Serum Albumin: Spectroscopic Approach”. Journal of Applied Biological Sciences 5/1 (February 2011), 35-47.
JAMA Teır MMA, Ghıthan JH, Darwısh SM, Abu-hadıd MM. Study of Progesterone interaction with Human Serum Albumin: Spectroscopic approach. J.appl.biol.sci. 2011;5:35–47.
MLA Teır, M. M. Abu et al. “Study of Progesterone Interaction With Human Serum Albumin: Spectroscopic Approach”. Journal of Applied Biological Sciences, vol. 5, no. 1, 2011, pp. 35-47.
Vancouver Teır MMA, Ghıthan JH, Darwısh SM, Abu-hadıd MM. Study of Progesterone interaction with Human Serum Albumin: Spectroscopic approach. J.appl.biol.sci. 2011;5(1):35-47.