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One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960

Year 2011, Volume: 5 Issue: 2, 61 - 65, 01.07.2011

Aytac Kocabas Didem Sutay Kocabas Ufuk Bakır Bolukbası

Abstract

The aim of study was to investigate purification and characterization of xylanase produced by industrially important strain of Aspergillus terreus. The xylanase was purified by one-step hydrophobic interaction chromatography technique 19-fold with 61% yield. Molecular weight and isoelectric point of the enzyme were determined as 19 kDa and pH 9.0, respectively. The enzyme was found to be unglycosylated. Kinetic experiments at 50°C and pH 7.0 resulted in apparent Km and Vmax values of 2.5±0.05 mg xylan/ml and 50.2±0.4 IU/µg protein, respectively. According to its biochemical properties, the enzyme was found to be a member of family-11 xylanase group. Due to its low molecular weight, the enzyme could be advantageous for industrial applications

Keywords

Aspergillus terreus xylanase hydrophobic interaction chromatography enzyme characterization

Year 2011, Volume: 5 Issue: 2, 61 - 65, 01.07.2011

Aytac Kocabas Didem Sutay Kocabas Ufuk Bakır Bolukbası

Abstract

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Details

Other ID JA25GD77EN
Journal Section Research Article
Authors

Aytac Kocabas This is me

Didem Sutay Kocabas This is me

Ufuk Bakır Bolukbası This is me

Publication Date July 1, 2011
Published in Issue Year 2011 Volume: 5 Issue: 2

Cite

APA Kocabas, A., Sutay Kocabas, D., & Bakır Bolukbası, U. (2011). One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960. Journal of Applied Biological Sciences, 5(2), 61-65.
AMA Kocabas A, Sutay Kocabas D, Bakır Bolukbası U. One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960. J.appl.biol.sci. July 2011;5(2):61-65.
Chicago Kocabas, Aytac, Didem Sutay Kocabas, and Ufuk Bakır Bolukbası. “One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus Terreus NRRL 1960”. Journal of Applied Biological Sciences 5, no. 2 (July 2011): 61-65.
EndNote Kocabas A, Sutay Kocabas D, Bakır Bolukbası U (July 1, 2011) One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960. Journal of Applied Biological Sciences 5 2 61–65.
IEEE A. Kocabas, D. Sutay Kocabas, and U. Bakır Bolukbası, “One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960”, J.appl.biol.sci., vol. 5, no. 2, pp. 61–65, 2011.
ISNAD Kocabas, Aytac et al. “One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus Terreus NRRL 1960”. Journal of Applied Biological Sciences 5/2 (July 2011), 61-65.
JAMA Kocabas A, Sutay Kocabas D, Bakır Bolukbası U. One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960. J.appl.biol.sci. 2011;5:61–65.
MLA Kocabas, Aytac et al. “One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus Terreus NRRL 1960”. Journal of Applied Biological Sciences, vol. 5, no. 2, 2011, pp. 61-65.
Vancouver Kocabas A, Sutay Kocabas D, Bakır Bolukbası U. One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960. J.appl.biol.sci. 2011;5(2):61-5.