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Evaluation and Optimization of Genomic DNA Isolation Protocols from Human Solid Tissues

Year 2011, Volume: 5 Issue: 2, 41 - 43, 01.07.2011

Nesrin Turaçlar Hasibe Cıngıllı Vural

Abstract

Molecular diagnostics are performed by using DNA isolated from different body tissues. However, it is necessary to obtain genomic DNA of good quality. The objective of this study was to describe the efficient protocol of DNA extraction from human breast, adipose, brain, liver, kidney, prostate, lung, larynx, endometrium and muscle tissues. We obtained high molecular weight DNA of good quality, shown by agarose gel and DNA fragments. Spectrophotometric analysis of DNA concentration showed variation among the DNA from different tissues, with the liver, breast, endometrium and adipose tissues presenting the greatest and the smallest concentration, respectively. The described protocol has proven to be advantageous due to its simplicity, quickness, affordable reagents and absence of phenol, resulting in a high molecular weight DNA of good quality from several tissues. Genomic DNA extraction from solid tissues of patients with cancer risks was carried out by using two different procedure including manuel and automatically isolation. The QIAamp spin columns (QIAGEN, Hilden, Germany) were used for extraction and purification of genomic DNA from different tissues by Tissue Kits and Tissue Card or automatically, EZ1 automatic DNA isolation system. Furthermore, we used Phenol-Chloroform protocol for manuel isolation in this study

Keywords

nDNA solid tissue

Year 2011, Volume: 5 Issue: 2, 41 - 43, 01.07.2011

Nesrin Turaçlar Hasibe Cıngıllı Vural

Abstract

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Details

Other ID JA98BG55ZK
Journal Section Research Article
Authors

Nesrin Turaçlar This is me

Hasibe Cıngıllı Vural This is me

Publication Date July 1, 2011
Published in Issue Year 2011 Volume: 5 Issue: 2

Cite

APA Turaçlar, N., & Cıngıllı Vural, H. (2011). Evaluation and Optimization of Genomic DNA Isolation Protocols from Human Solid Tissues. Journal of Applied Biological Sciences, 5(2), 41-43.
AMA Turaçlar N, Cıngıllı Vural H. Evaluation and Optimization of Genomic DNA Isolation Protocols from Human Solid Tissues. J.appl.biol.sci. July 2011;5(2):41-43.
Chicago Turaçlar, Nesrin, and Hasibe Cıngıllı Vural. “Evaluation and Optimization of Genomic DNA Isolation Protocols from Human Solid Tissues”. Journal of Applied Biological Sciences 5, no. 2 (July 2011): 41-43.
EndNote Turaçlar N, Cıngıllı Vural H (July 1, 2011) Evaluation and Optimization of Genomic DNA Isolation Protocols from Human Solid Tissues. Journal of Applied Biological Sciences 5 2 41–43.
IEEE N. Turaçlar and H. Cıngıllı Vural, “Evaluation and Optimization of Genomic DNA Isolation Protocols from Human Solid Tissues”, J.appl.biol.sci., vol. 5, no. 2, pp. 41–43, 2011.
ISNAD Turaçlar, Nesrin - Cıngıllı Vural, Hasibe. “Evaluation and Optimization of Genomic DNA Isolation Protocols from Human Solid Tissues”. Journal of Applied Biological Sciences 5/2 (July 2011), 41-43.
JAMA Turaçlar N, Cıngıllı Vural H. Evaluation and Optimization of Genomic DNA Isolation Protocols from Human Solid Tissues. J.appl.biol.sci. 2011;5:41–43.
MLA Turaçlar, Nesrin and Hasibe Cıngıllı Vural. “Evaluation and Optimization of Genomic DNA Isolation Protocols from Human Solid Tissues”. Journal of Applied Biological Sciences, vol. 5, no. 2, 2011, pp. 41-43.
Vancouver Turaçlar N, Cıngıllı Vural H. Evaluation and Optimization of Genomic DNA Isolation Protocols from Human Solid Tissues. J.appl.biol.sci. 2011;5(2):41-3.