Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants

Wafaa Kamal El-Din Hegazy; Sanaa Elsayed Ahmed Tork; Mohamed Safwat Abdel-salam

Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants

Year 2012, Volume: 6 Issue: 1, 25 - 30, 01.02.2012

Wafaa Kamal El-Din Hegazy Sanaa Elsayed Ahmed Tork Mohamed Safwat Abdel-salam

Abstract

This work aimed to characterize recombinant strains, with a high production level of L-asparaginase, previously obtained by protoplast fusion between Bacillus subtilis and Bacillus cereus. The genetic relationship between fusants and their parents was determined using a simple PCRrestriction fragment length polymorphism (RFLP) of 16S rDNAs -based method, and by analyzing the specifi c L-asparaginase genes and protein patterns. Our results indicate that all fusants have morphological characteristics and specifi c L-asparaginase genes similar to the Bacillus cereus strain whereas protein pattern and 16S rDNAs -RFLP analysis show mixed characteristics from both parents. Different genetic relationships were found between each fusant and its parental strain. These results confi rm that protoplast fusion is an important technique in strain improvement. It was used to combine genes from different organisms for creating strains with desired properties

Keywords

RFLP, Fusants, 16S rDNA, SDS – PAGE

Year 2012, Volume: 6 Issue: 1, 25 - 30, 01.02.2012

Wafaa Kamal El-Din Hegazy Sanaa Elsayed Ahmed Tork Mohamed Safwat Abdel-salam

Abstract

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Details

Other ID	JA75CB32NR
Journal Section	Research Article
Authors	Wafaa Kamal El-Din Hegazy This is me Sanaa Elsayed Ahmed Tork This is me Mohamed Safwat Abdel-salam This is me
Publication Date	February 1, 2012
Published in Issue	Year 2012 Volume: 6 Issue: 1

Cite

APA	Hegazy, W. K. E.-D., Tork, S. E. A., & Abdel-salam, M. S. (2012). Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants. Journal of Applied Biological Sciences, 6(1), 25-30.
AMA	Hegazy WKED, Tork SEA, Abdel-salam MS. Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants. J.appl.biol.sci. February 2012;6(1):25-30.
Chicago	Hegazy, Wafaa Kamal El-Din, Sanaa Elsayed Ahmed Tork, and Mohamed Safwat Abdel-salam. “Molecular Characterization of Overproducing L-Asparaginase Recombinant Bacillus Fusants”. Journal of Applied Biological Sciences 6, no. 1 (February 2012): 25-30.
EndNote	Hegazy WKE-D, Tork SEA, Abdel-salam MS (February 1, 2012) Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants. Journal of Applied Biological Sciences 6 1 25–30.
IEEE	W. K. E.-D. Hegazy, S. E. A. Tork, and M. S. Abdel-salam, “Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants”, J.appl.biol.sci., vol. 6, no. 1, pp. 25–30, 2012.
ISNAD	Hegazy, Wafaa Kamal El-Din et al. “Molecular Characterization of Overproducing L-Asparaginase Recombinant Bacillus Fusants”. Journal of Applied Biological Sciences 6/1 (February 2012), 25-30.
JAMA	Hegazy WKE-D, Tork SEA, Abdel-salam MS. Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants. J.appl.biol.sci. 2012;6:25–30.
MLA	Hegazy, Wafaa Kamal El-Din et al. “Molecular Characterization of Overproducing L-Asparaginase Recombinant Bacillus Fusants”. Journal of Applied Biological Sciences, vol. 6, no. 1, 2012, pp. 25-30.
Vancouver	Hegazy WKE-D, Tork SEA, Abdel-salam MS. Molecular Characterization of Overproducing L-asparaginase Recombinant Bacillus Fusants. J.appl.biol.sci. 2012;6(1):25-30.