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Isolation of Cold Shock Protein profilles from Pseudomonas sp. Strains and Analyses of SDS-PAGE

Year 2013, Volume: 7 Issue: 2, 33 - 36, 01.07.2013

Zuhal Uzumcu Yasemin Caf Ebrahim Valıpour Yelis Maasoglu Burhan Arıkan

Abstract

In this research, Pseudomonas aeruginosa balcalı-1, Pseudomonas aeruginosa balcalı-2 which taken Çukurova Univercity Balcalı Hospital Laboratory for cold shock protein isolation and Pseudomonas aeruginosa ATCC 27853 strains were used. For cold shock protein synthesis had choosen 20, 10, 5 ºC and the temperature values selected for 60 and 480 minutes incubation were carried out. Cold shock proteins to determine the molecular weight, SDS-PAGE gel system was used. P. aeruginosa ATCC 27853 strain isolated in 1700 and 18130Da weight proteins, were synthesized more than other proteins. These two proteins have occuranced with the cold shock treatment. P. aeruginosa Balcalı 1 produced the protein in 77066Da cold shock protein at 20ºC at for 60minutes and 5, 10, 20ºC for 480 minutes incubation SDS-PAGE analysis showed that P. aeruginosa Balcalı 2 strains numbered two different protein bands in 22666and 21576 Dalton molecular weight. Strains used in the study of Pseudomonas balcalı-1 and balcalı-2 from Cold Shock response higher constituted than Pseudomonas ATCC 27853. As a conclusion, Pseudomonas have three different strains Cold shock treatment when isolated the most cold shock protein, 10 ºC at for 60 minutes incubation

Keywords

Pseudomonas aeruginosa. Cold Shock Protein SDS-PAGE

Year 2013, Volume: 7 Issue: 2, 33 - 36, 01.07.2013

Zuhal Uzumcu Yasemin Caf Ebrahim Valıpour Yelis Maasoglu Burhan Arıkan

Abstract

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Details

Other ID JA45CZ58AR
Journal Section Collection
Authors

Zuhal Uzumcu This is me

Yasemin Caf This is me

Ebrahim Valıpour This is me

Yelis Maasoglu This is me

Burhan Arıkan This is me

Publication Date July 1, 2013
Published in Issue Year 2013 Volume: 7 Issue: 2

Cite

APA Uzumcu, Z., Caf, Y., Valıpour, E., Maasoglu, Y., et al. (2013). Isolation of Cold Shock Protein profilles from Pseudomonas sp. Strains and Analyses of SDS-PAGE. Journal of Applied Biological Sciences, 7(2), 33-36.
AMA Uzumcu Z, Caf Y, Valıpour E, Maasoglu Y, Arıkan B. Isolation of Cold Shock Protein profilles from Pseudomonas sp. Strains and Analyses of SDS-PAGE. J.appl.biol.sci. July 2013;7(2):33-36.
Chicago Uzumcu, Zuhal, Yasemin Caf, Ebrahim Valıpour, Yelis Maasoglu, and Burhan Arıkan. “Isolation of Cold Shock Protein Profilles from Pseudomonas Sp. Strains and Analyses of SDS-PAGE”. Journal of Applied Biological Sciences 7, no. 2 (July 2013): 33-36.
EndNote Uzumcu Z, Caf Y, Valıpour E, Maasoglu Y, Arıkan B (July 1, 2013) Isolation of Cold Shock Protein profilles from Pseudomonas sp. Strains and Analyses of SDS-PAGE. Journal of Applied Biological Sciences 7 2 33–36.
IEEE Z. Uzumcu, Y. Caf, E. Valıpour, Y. Maasoglu, and B. Arıkan, “Isolation of Cold Shock Protein profilles from Pseudomonas sp. Strains and Analyses of SDS-PAGE”, J.appl.biol.sci., vol. 7, no. 2, pp. 33–36, 2013.
ISNAD Uzumcu, Zuhal et al. “Isolation of Cold Shock Protein Profilles from Pseudomonas Sp. Strains and Analyses of SDS-PAGE”. Journal of Applied Biological Sciences 7/2 (July 2013), 33-36.
JAMA Uzumcu Z, Caf Y, Valıpour E, Maasoglu Y, Arıkan B. Isolation of Cold Shock Protein profilles from Pseudomonas sp. Strains and Analyses of SDS-PAGE. J.appl.biol.sci. 2013;7:33–36.
MLA Uzumcu, Zuhal et al. “Isolation of Cold Shock Protein Profilles from Pseudomonas Sp. Strains and Analyses of SDS-PAGE”. Journal of Applied Biological Sciences, vol. 7, no. 2, 2013, pp. 33-36.
Vancouver Uzumcu Z, Caf Y, Valıpour E, Maasoglu Y, Arıkan B. Isolation of Cold Shock Protein profilles from Pseudomonas sp. Strains and Analyses of SDS-PAGE. J.appl.biol.sci. 2013;7(2):33-6.