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Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes

Year 2013, Volume: 7 Issue: 3, 51 - 55, 01.11.2013

Sezai Türkel Nükhet Kayakent

Abstract

Six different new Kluyveromyces lactis strains were isolated from different dairy products from Bursa province, Turkey. Lactase and invertase enzyme activities of new K. lactis strains were determined under different growth conditions. Our results indicate that both lactase and invertase enzyme activities of new K. lactis strains are much higher than the standard K. lactis (ATCC8585). Lactase and invertase enzymes are expressed at high levels under glucose repressed growth conditions in some of these K. lactis strains. Invertase activities of new K. lactis strains are 10-fold higher than the standard K. lactis (ATCC8585) strain. Additionally, it was shown that the two of the new K. lactis strains have 25% shorter duplication times than the normal K. lactis (ATCC8585). Our results indicate that new K. lactis strains isolated from the local dairy products of the Bursa region of Turkey have distinct metabolic features with respect to the regulation of GAL/LAC regulon of K. lactis

Keywords

Kluyveromyces lactis Lactase Invertase Yeast identification PCR-RFLP

Year 2013, Volume: 7 Issue: 3, 51 - 55, 01.11.2013

Sezai Türkel Nükhet Kayakent

Abstract

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Details

Other ID JA32ZN53NK
Journal Section Research Article
Authors

Sezai Türkel This is me

Nükhet Kayakent This is me

Publication Date November 1, 2013
Published in Issue Year 2013 Volume: 7 Issue: 3

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APA Türkel, S., & Kayakent, N. (2013). Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. Journal of Applied Biological Sciences, 7(3), 51-55.
AMA Türkel S, Kayakent N. Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. J.appl.biol.sci. November 2013;7(3):51-55.
Chicago Türkel, Sezai, and Nükhet Kayakent. “Isolation and Molecular Identification of New Kluyveromyces Lactis Strains Producing High Levels of Lactase and Invertase Enzymes”. Journal of Applied Biological Sciences 7, no. 3 (November 2013): 51-55.
EndNote Türkel S, Kayakent N (November 1, 2013) Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. Journal of Applied Biological Sciences 7 3 51–55.
IEEE S. Türkel and N. Kayakent, “Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes”, J.appl.biol.sci., vol. 7, no. 3, pp. 51–55, 2013.
ISNAD Türkel, Sezai - Kayakent, Nükhet. “Isolation and Molecular Identification of New Kluyveromyces Lactis Strains Producing High Levels of Lactase and Invertase Enzymes”. Journal of Applied Biological Sciences 7/3 (November 2013), 51-55.
JAMA Türkel S, Kayakent N. Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. J.appl.biol.sci. 2013;7:51–55.
MLA Türkel, Sezai and Nükhet Kayakent. “Isolation and Molecular Identification of New Kluyveromyces Lactis Strains Producing High Levels of Lactase and Invertase Enzymes”. Journal of Applied Biological Sciences, vol. 7, no. 3, 2013, pp. 51-55.
Vancouver Türkel S, Kayakent N. Isolation and Molecular Identification of New Kluyveromyces lactis Strains Producing High Levels of Lactase and Invertase Enzymes. J.appl.biol.sci. 2013;7(3):51-5.