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Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method

Year 2015, Volume: 9 Issue: 1, 54 - 56, 01.02.2015

Fereshteh Motamedı Jalil Fallah Mehrabadı Azar Sabokbar

Abstract

Background: Listeria monocytogenes is a gram positive and facultative anaerobic bacteria and Listeria species have a high spread in environment. Listeria monocytogenes is agent of meningitides, encephalitis and septicemia and could accrue during pregnancy. Detection of Listeria via culture method is difficult. The aim of this study was accessing of Listeria monocytogenes in pregnant women who refer to women hospital of Tehran via culture and Real Time PCR methods. Methods: Two hundred cervix swabs samples were collected from pregnant women in seventh and eights mounts of pregnancy and transfer to laboratory in 2ml transport medium. 1ml was used for DNA extracting directly and other keep in refrigerator for cold enrichment among 4 weeks and then cultured. Primers were designed for amplifying hly gene which coded listeriolysin O. Results: The result of culture and real time PCR methods were showed of 200 samples, 28 (14%) and 41(20.5%) were positive, respectively. Conclusion: All of 13 false negative via culture, were positive in PCR technique. So, molecular method was more sensitive and recommended for applying. Moreover, the results showed high prevalence of carrier women in this time. Therefore, public screening was recommended for pregnant women

Keywords

Listeria monocytogenes culture molecular detection

Year 2015, Volume: 9 Issue: 1, 54 - 56, 01.02.2015

Fereshteh Motamedı Jalil Fallah Mehrabadı Azar Sabokbar

Abstract

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Details

Other ID JA35CA45ZM
Journal Section Research Article
Authors

Fereshteh Motamedı This is me

Jalil Fallah Mehrabadı This is me

Azar Sabokbar This is me

Publication Date February 1, 2015
Published in Issue Year 2015 Volume: 9 Issue: 1

Cite

APA Motamedı, F., Mehrabadı, J. F., & Sabokbar, A. (2015). Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method. Journal of Applied Biological Sciences, 9(1), 54-56.
AMA Motamedı F, Mehrabadı JF, Sabokbar A. Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method. J.appl.biol.sci. February 2015;9(1):54-56.
Chicago Motamedı, Fereshteh, Jalil Fallah Mehrabadı, and Azar Sabokbar. “Discovery of Listeria Monocytogenes High Prevalence in Pregnant Women Who Referred to the Tehran Women Reference Hospital by Real Time PCR Method”. Journal of Applied Biological Sciences 9, no. 1 (February 2015): 54-56.
EndNote Motamedı F, Mehrabadı JF, Sabokbar A (February 1, 2015) Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method. Journal of Applied Biological Sciences 9 1 54–56.
IEEE F. Motamedı, J. F. Mehrabadı, and A. Sabokbar, “Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method”, J.appl.biol.sci., vol. 9, no. 1, pp. 54–56, 2015.
ISNAD Motamedı, Fereshteh et al. “Discovery of Listeria Monocytogenes High Prevalence in Pregnant Women Who Referred to the Tehran Women Reference Hospital by Real Time PCR Method”. Journal of Applied Biological Sciences 9/1 (February 2015), 54-56.
JAMA Motamedı F, Mehrabadı JF, Sabokbar A. Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method. J.appl.biol.sci. 2015;9:54–56.
MLA Motamedı, Fereshteh et al. “Discovery of Listeria Monocytogenes High Prevalence in Pregnant Women Who Referred to the Tehran Women Reference Hospital by Real Time PCR Method”. Journal of Applied Biological Sciences, vol. 9, no. 1, 2015, pp. 54-56.
Vancouver Motamedı F, Mehrabadı JF, Sabokbar A. Discovery of Listeria monocytogenes High Prevalence in Pregnant Women who referred to the Tehran Women Reference Hospital by Real Time PCR Method. J.appl.biol.sci. 2015;9(1):54-6.