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Production of Cold Active Lipase from Bacillus sp.

Year 2017, Volume: 11 Issue: 2, 24 - 27, 31.08.2017

Abstract

A cold active lipase producing Bacillus sp. strains were isolated from sewage of oil. Bacillus sp. strain SY-7 was determined as the best lipase producing isolate. The highest enzyme production was found at 20°C and pH 8.0 on tributyrin media. Analyses of molecular mass of the partial purified lipase was carried out by SDS-PAGE which revealed a single band as 110.5 kDa. The enzyme activity and stability were determined by spectrophotometric and titrimetric methods. The enzyme was active between pH 4.0-10.0 and 5-50°C and showed optimal activity and stability at pH 8.0 and 20°C. In the presence of BaCl2 (4mM), KCl (4mM), AgNO3 (4mM), CuSO4 (4mM), MgCl2 (4mM), CaCl2 (4mM), ZnCl2 (4mM) and NaCl (4mM), the enzyme exhibited the following activities 105%, 100%, 100%, 89%, 105%, 95%, 100% and 100%, respectively. In the presence of Tween-20(5%), Tween-80(5%), detergent-1(5%) and detergent-6(5%) the enzyme saved its original activity. SDS(5%), detergent-2(5%) and detergent-7(5%) increased the activity 10%, 5% and 5%, respectively. Detergent-3(5%), detergent-4(5%) and detergent-5(5%) reduced its activity respectively, 14%, 14% and 52%. According to these results, SY-7 lipase shows alkaline, psychrotrophilic, cold active and stable, chelator and detergent resistant properties. Owing to these properties, this lipase can be useful in detergent industry.

References

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  • [3] Gökbulut and Arslanoğlu, 2013. Purification and biochemical characterization of an extracellular lipase from psychrotolerant Pseudomonas fluorescens KE38. Turk J Biol 37:538-546.
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  • [5] Pascale, D., Cusano, A.M., Autore, F., Parilli, E., Prisco, G., Marino, G. and Tutino, M.L., 2008. The cold-active Lip1 lipase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 is a member of a new bacterial lipolytic enzyme family. Extremophiles 12: 311-323.
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  • [9] Kamini, N. R., Fujii, T., Kurosu, T. and Lefuji, H., 2000. Production, purification and characterization of an extracellular lipase from the yeast, Cryptococcus sp. S-2. Process Biochem, 36: 317-324.
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  • [12] Arabacı N., 2011. Isolation of cold act,ve alkaline amylase producıng Bacillus sp. strains, enzyme production, characterization and investigation of industrial applications. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Yüksek Lisans Tezi (Biyoloji Anabilim Dalı).
  • [13] Rahman, R.N.Z.R.A., Baharum, S.N., Basri, M. and Salleh, A.B., 2005. High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5. Analytical Biochem, 341: 267-274.
  • [14] Tanrısever, D., 2011. Bacillus subtilis’ten termostabil lipaz üretimi ve karakterizasyonu. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Yüksek Lisans Tezi (Biyoteknoloji Anabilim Dalı).
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  • [17] Rashid, N., Shimada, Y., Ezaki, S., Atomi, H. and Imanaka, T., 2001. Low Temperature Lipase from Psychrotrophic Pseudomonas sp. Strain KB700A. Apllied and Enviromental Microbiology, 67(9)-4064.
  • [18] Jeon, J.H., Kim, J.T., Kim, Y.J., Kim, H.K., Lee, H.S., Kang, S.G., Kim, S.J. and Lee, J.H., 2009. Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome. Appl Microbiol Biotechnol, 81: 865-874.
  • [19] Hasan, F., Shah, A.A. and Hameed, A., 2009. Methods for detection and characterization of lipases. A comprehensive review. Biotechnology Advances, 27: 782-798.
  • [20] Yu, E.Y., Kwon, M.A., Lee, M., Oh, J.Y., Choi, J.E., Lee, J.Y., Song, B.K., Hahm, D.H. and Song, J.K., 2011. Isolation and characterization of a cold-active family VIII esterases from an arctic soil metagenome. Appl Microbiol Biotechnol, 90: 573-581.
  • [21] Ertuğrul, S., Dönmez, G. Andtakaç, S., 2007. Isolation of lipase producing Bacillus sp. from olive mill wastewater and improving its enzyme activity. Journal of Hazardous Materials, 149: 720-724.
  • [22] Kiran, G.S., Shanmughaprıya, S., Jayalakshimi, J., Selvin, J., Gandhimathi, R., Sıvaramakrishnan, S., Arunkumar, M., Thangavelu, T. and Natarajaseenivasan, K., 2008. Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp. (MSI057). Bioprocess Biosyst Eng, 31: 483-492.
  • [23] Chen, R., Guo, N.L. and Dang, H., 2011. Gene cloning, expression and characterization of a cold-adapted lipase from a psychrophilic deep-sea bacterium Psychrobacter sp. C18. World J Microbiol Biotechnol, 27: 431-441.
  • [24] Liu, R., Jiang, X., Mou, H., Guan, H., Hwang, H. and Li, X., 2009. A novel low-temperature resistant alkaline lipase from a soda lake fungus strain Fusarium solani N4-2 for detergent formulation. Biochemical Engineering Journal, 46: 265-270.
Year 2017, Volume: 11 Issue: 2, 24 - 27, 31.08.2017

Abstract

References

  • [1] Eltaweel, M.A., Rahman, R.N.Z.R.A., Salleh, A.B. and Basri, M., 2005. An organic solvent-stable lipase from Bacillus sp. strain 42. Annals of Microbiology, 55: 187-192.
  • [2] Joseph, B., Ramteke, P.W. and Thomas, G., 2008. Cold active lipases: Some hot issues and recent developments. Biotechnology Advances, 26: 457-470.
  • [3] Gökbulut and Arslanoğlu, 2013. Purification and biochemical characterization of an extracellular lipase from psychrotolerant Pseudomonas fluorescens KE38. Turk J Biol 37:538-546.
  • [4] Nardini, M. and Dijkstra, B.W., 1999. α/β Hydrolase fold enzymes: the family keeps growing. Current Opinion in Structural Biology, 9: 732-737.
  • [5] Pascale, D., Cusano, A.M., Autore, F., Parilli, E., Prisco, G., Marino, G. and Tutino, M.L., 2008. The cold-active Lip1 lipase from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 is a member of a new bacterial lipolytic enzyme family. Extremophiles 12: 311-323.
  • [6] Reetz, M.T., 2002. Lipases as practical biocatalysts. Current Opinion in Chemcial Biology, 6: 145-150.
  • [7] Jaeger, K.E. and Reetz, M.T., 2000. Directed evolution of enantioselectivite enzymes for organic chemistry. Curr Opin Chem Biol, 4: 68-73.
  • [8] Bell, P.J., Sunna, A., Gibbs M.D., Curach, N.C., Nevalainen, H. and Bergquist, P.L., 2002. Prospecting for novel lipase genes using PCR. Microbiology, 148: 2283-2291.
  • [9] Kamini, N. R., Fujii, T., Kurosu, T. and Lefuji, H., 2000. Production, purification and characterization of an extracellular lipase from the yeast, Cryptococcus sp. S-2. Process Biochem, 36: 317-324.
  • [10] Jeager, K.E. and Eggert, T., 2002. Lipases for biotechnology. Curr Opin Biotechnol, 13(4): 390-7
  • [11] Hasan F, Shah A., Hameed A. 2006. Industrial applications of microbial lipases. Enzyme and Microbiol Technology, 39:235-251.
  • [12] Arabacı N., 2011. Isolation of cold act,ve alkaline amylase producıng Bacillus sp. strains, enzyme production, characterization and investigation of industrial applications. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Yüksek Lisans Tezi (Biyoloji Anabilim Dalı).
  • [13] Rahman, R.N.Z.R.A., Baharum, S.N., Basri, M. and Salleh, A.B., 2005. High-yield purification of an organic solvent-tolerant lipase from Pseudomonas sp. strain S5. Analytical Biochem, 341: 267-274.
  • [14] Tanrısever, D., 2011. Bacillus subtilis’ten termostabil lipaz üretimi ve karakterizasyonu. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Yüksek Lisans Tezi (Biyoteknoloji Anabilim Dalı).
  • [15] Temizkan, G. and Arda, N., 2008. Moleküler Biyolojide Kullanılan Yöntemler. 3. Baskı, Nobel Tıp Kitabevi, İstanbul.
  • [16] Wiley, J. and Sons (2001). Current Protocols in Food Analytical Chemistry. C3.1.1-C3.1.13.
  • [17] Rashid, N., Shimada, Y., Ezaki, S., Atomi, H. and Imanaka, T., 2001. Low Temperature Lipase from Psychrotrophic Pseudomonas sp. Strain KB700A. Apllied and Enviromental Microbiology, 67(9)-4064.
  • [18] Jeon, J.H., Kim, J.T., Kim, Y.J., Kim, H.K., Lee, H.S., Kang, S.G., Kim, S.J. and Lee, J.H., 2009. Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome. Appl Microbiol Biotechnol, 81: 865-874.
  • [19] Hasan, F., Shah, A.A. and Hameed, A., 2009. Methods for detection and characterization of lipases. A comprehensive review. Biotechnology Advances, 27: 782-798.
  • [20] Yu, E.Y., Kwon, M.A., Lee, M., Oh, J.Y., Choi, J.E., Lee, J.Y., Song, B.K., Hahm, D.H. and Song, J.K., 2011. Isolation and characterization of a cold-active family VIII esterases from an arctic soil metagenome. Appl Microbiol Biotechnol, 90: 573-581.
  • [21] Ertuğrul, S., Dönmez, G. Andtakaç, S., 2007. Isolation of lipase producing Bacillus sp. from olive mill wastewater and improving its enzyme activity. Journal of Hazardous Materials, 149: 720-724.
  • [22] Kiran, G.S., Shanmughaprıya, S., Jayalakshimi, J., Selvin, J., Gandhimathi, R., Sıvaramakrishnan, S., Arunkumar, M., Thangavelu, T. and Natarajaseenivasan, K., 2008. Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp. (MSI057). Bioprocess Biosyst Eng, 31: 483-492.
  • [23] Chen, R., Guo, N.L. and Dang, H., 2011. Gene cloning, expression and characterization of a cold-adapted lipase from a psychrophilic deep-sea bacterium Psychrobacter sp. C18. World J Microbiol Biotechnol, 27: 431-441.
  • [24] Liu, R., Jiang, X., Mou, H., Guan, H., Hwang, H. and Li, X., 2009. A novel low-temperature resistant alkaline lipase from a soda lake fungus strain Fusarium solani N4-2 for detergent formulation. Biochemical Engineering Journal, 46: 265-270.
There are 24 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Sara Yasemin

Nihan Arabacı This is me

Hatice Korkmaz Güvenmez

Publication Date August 31, 2017
Published in Issue Year 2017 Volume: 11 Issue: 2

Cite

APA Yasemin, S., Arabacı, N., & Korkmaz Güvenmez, H. (2017). Production of Cold Active Lipase from Bacillus sp. Journal of Applied Biological Sciences, 11(2), 24-27.
AMA Yasemin S, Arabacı N, Korkmaz Güvenmez H. Production of Cold Active Lipase from Bacillus sp. J.appl.biol.sci. August 2017;11(2):24-27.
Chicago Yasemin, Sara, Nihan Arabacı, and Hatice Korkmaz Güvenmez. “Production of Cold Active Lipase from Bacillus Sp”. Journal of Applied Biological Sciences 11, no. 2 (August 2017): 24-27.
EndNote Yasemin S, Arabacı N, Korkmaz Güvenmez H (August 1, 2017) Production of Cold Active Lipase from Bacillus sp. Journal of Applied Biological Sciences 11 2 24–27.
IEEE S. Yasemin, N. Arabacı, and H. Korkmaz Güvenmez, “Production of Cold Active Lipase from Bacillus sp”., J.appl.biol.sci., vol. 11, no. 2, pp. 24–27, 2017.
ISNAD Yasemin, Sara et al. “Production of Cold Active Lipase from Bacillus Sp”. Journal of Applied Biological Sciences 11/2 (August 2017), 24-27.
JAMA Yasemin S, Arabacı N, Korkmaz Güvenmez H. Production of Cold Active Lipase from Bacillus sp. J.appl.biol.sci. 2017;11:24–27.
MLA Yasemin, Sara et al. “Production of Cold Active Lipase from Bacillus Sp”. Journal of Applied Biological Sciences, vol. 11, no. 2, 2017, pp. 24-27.
Vancouver Yasemin S, Arabacı N, Korkmaz Güvenmez H. Production of Cold Active Lipase from Bacillus sp. J.appl.biol.sci. 2017;11(2):24-7.