Production of Cold Active Lipase from Bacillus sp.

Year 2017, Volume: 11 Issue: 2, 24 - 27, 31.08.2017

Sara Yasemin , Nihan Arabacı Hatice Korkmaz Güvenmez

Abstract

A cold active lipase producing Bacillus sp. strains were isolated from sewage of oil. Bacillus sp. strain SY-7 was determined as the best lipase producing isolate. The highest enzyme production was found at 20°C and pH 8.0 on tributyrin media. Analyses of molecular mass of the partial purified lipase was carried out by SDS-PAGE which revealed a single band as 110.5 kDa. The enzyme activity and stability were determined by spectrophotometric and titrimetric methods. The enzyme was active between pH 4.0-10.0 and 5-50°C and showed optimal activity and stability at pH 8.0 and 20°C. In the presence of BaCl2 (4mM), KCl (4mM), AgNO3 (4mM), CuSO4 (4mM), MgCl2 (4mM), CaCl2 (4mM), ZnCl2 (4mM) and NaCl (4mM), the enzyme exhibited the following activities 105%, 100%, 100%, 89%, 105%, 95%, 100% and 100%, respectively. In the presence of Tween-20(5%), Tween-80(5%), detergent-1(5%) and detergent-6(5%) the enzyme saved its original activity. SDS(5%), detergent-2(5%) and detergent-7(5%) increased the activity 10%, 5% and 5%, respectively. Detergent-3(5%), detergent-4(5%) and detergent-5(5%) reduced its activity respectively, 14%, 14% and 52%. According to these results, SY-7 lipase shows alkaline, psychrotrophilic, cold active and stable, chelator and detergent resistant properties. Owing to these properties, this lipase can be useful in detergent industry.

Keywords

Lipase, psychrophilic, alkaline, detergent resistant, Bacillus sp. SY-7

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