In vitro high frequency axillary shoot regeneration of Roundleaf toothcup-Rotala rotundifolia [(BuchHam. ex Roxb) Koehne]

Year 2017, Volume: 11 Issue: 2, 44 - 47, 31.08.2017

Mehmet Karataş Muzaffer Çiftçioğlu Muhammad Aasim

Abstract

Roundleaf toothcup-Rotala rotundifolia [(BuchHam. ex Roxb) Koehne] is an amphibious aquatic plant of Southeast Asia used
as medicinal and ornamental purpose. The shoot tip explant of R. rotundifolia was aseptically excised and cultured on MS
(Murashige and Skoog) medium enriched with 0.05-1.60 mg/l Thidiazuron (TDZ) that resulted in callus induction without any
shoot induction after 8 weeks of inoculation. Thereafter, the explants were transferred to MSO for further 4 weeks that resulted
in 100% shoot regeneration frequency, 29.33-59.67 shoots per explant alongwith mean shoot length ranged 0.20-1.87 cm.
Maximum number of 59.67 shoot alongwith mean longer shoots (1.87 cm) were achieved on MS medium with 0.05 mg/l TDZ.
Further increase of TDZ concentration in the culture medium inhibited the both shoots per explant and shoot length. Soots with
≈1.0 cm length were successfully rooted on medium enriched with 0.25 mg/l Indole-3-butyric Acid (IBA) that was followed by
successfıul adaptation of plantlets in the aquariums filled with tap water. The protocol developed can be employed for future
biotechnological studies like genetic transformation. 

Keywords

Axillary shoots, In vitro, Shoot tip, Thidiazuron

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