Biodegradation of Diesel by Acinetobacter lwoffii and Vibrio alginolyticus Isolated from Ship Dismantling Facility in Tanjungjati Coast, Madura, Indonesia

Year 2018, Volume: 12 Issue: 1, 1 - 8, 31.07.2018

Muhammad Fauzul Imron Harmin Sulistiyaning Tıtah

Abstract

Presence of diesel in environment will cause serious effects to ecosystembecause the components of diesel are potentially carcinogenic and toxic for environment. Diesel contaminated seawater and soil samples were collected from ship dismantling facility in Tanjungjati coast, Madura, Indonesia.Isolation of diesel degrading bacteria was performed using serial dilution method.Preliminary and secondary screening of diesel degrading bacteria were carried out by using nutrient agar medium and Mineral Salt Medium (MSM) containing diesel (0; 5; 10; and 15% (v/v))as a carbon source.The diesel degradation was conducted by inoculating bacteria that potentially degrade diesel into MSM with optimum concentration and diesel degradation percentage was detected by GC-MS analysis.In this study, thirteen bacteria strains were isolated which had the potential to degrade diesel. Based on preliminary and secondary screening, the optimum concentration was found to be 10% (v/v) of diesel and bacteria coded AT and EL have highest growth with 8.01 and 8.13 log CFU/mL for 7 days incubation. Bacterial code of AT and EL were identified as Acinetobacter lwoffii and Vibrio alginolyticus. The result showed that V. alginolyticus and A. lwoffii were able to degrade diesel about 26.78% and 1.85% for 14 days of incubation at initial diesel of 10% (v/v). In conclusion, V. alginolyticus may has a potential as an degrader for the bioremediation of marine environments polluted by dieselwith further investigation through biostimulation and bioaugmentation technique.

Keywords

Acinetobacter lwoffii, Biodegradation, Diesel, Ship dismantling facility, Vibrio alginolyticus

References

• Abdel-El-Haleem, D (2003) Acinetobacter: Environmental and Biotechnological Applications. African Journal of Biotechnology, 2(4): 71 – 74
• AlDisi, Z., Jaoua, S., Al-Thani, D., AlMeer, S., and Zouari, N (2016) Isolation, Screening and Activity of Hydrocarbon Degrading Bacteria from Harsh Soils. Proceedings of the World Congress on Civil, Structural, and Environmental Engineering, Prague, Czech Republic, March 30 – 31, 2016. Doi: 10.11159/icesdp16.104
• Bhasheer, S.K., Umavathi, S., Banupriya, D., Thangavel, M., and Thangam, Y (2014) Diversity of Diesel Degrading Bacteria from a Hydrocarbon Contaminated Soil. Int.J.Curr.Microbiol.App.Sci, 3(11): 363 – 369
• Bhuvaneswar, C., Swathi, G., Bhaskar, B. V., Munichandrababu, T., and Rajendra, W (2012) Effective Synergetic Biodegradation of Diesel Oil by Bacteria. International Journal of Environmental Biology, 2(4): 195-199
• Dadrasnia, A. and Agamuthu, P (2013) Diesel Fuel Degradation from Contaminated Soil by Dracaena reflexa using Organic Waste Supplementation. Journal of the Japan Petroleum Institute, 56(4): 236 – 243
• Deepika KV and Bramhachari PV (2015) Biodegradation of petroleum hydrocarbons using microbial consortium isolated from mangrove sediments of Krishna river delta. New Horizons in Biotechnology, 38 – 40
• Deng M. C., Li J., Liang F. R., Yi M., Xu X.M., Yuan J.P., Peng J., Wu C. F., and wang J. H (2014) Isolation and Characterization of a Novel Hydrocarbon Degrading Bacterium Achromobacer sp. HZ01 from the Crude Oil Contaminated Seawater an the Daya Bay, Southern China. Marine Pollution Bulletin, 83(1): 79 – 86
• Hamzah, A., Rabu, A., Azmy, R.F.H.R., and Yussoff, N.A (2010) Isolation and Characterization of Bacteria Degrading Sumandak and South Angsi Oils. Sains Malaysiana 39(2): 161–168
• Hu, X., Caixia Wang, and Peng Wang (2015) Optimization and characterization of biosurfactant production from marine Vibrio sp. Strain 3B-2. Front Microbiol, 6(976): 1 – 13
• Ibrahim, H. M. M (2016) Biodegradation of used Engine Oil by Novel Strains of Ochrobactrum anthropi HM-1 and Citrobacter freundii HM-2 Isolated from Oil-Contaminated Soil. 3 Biotech, 3(226): 1 – 13
• Isiodu G.G., Stanley H.O., Ezebuiro V., and Okerentugba P.O (2016) Role of Plasmid-Borne Genes in the Biodegradation of Polycyclic Aromatic Hydrocarbons (PAHs) by Consortium of Aerobic Heterotrophic Bacteria. Petroleum & Environmental Biotechnology, 7(1): 1 – 7
• Luo Q, Xian-Rong S, Jian-Guo Z, Zheng-Qiu F, and Ying H(2012) Isolation, identification and biodegradation ability of diesel oil degrading Pseudomonas sp. Strain C7 from bilge water. Afr J Microbiol, 6(5):1033–1040.
• Maharani, W.R., Setiyono, H., and Setyawan, W.B (2014) Studi Distribusi Suhu, Salinitas dan Densitas Secara Vertikal dan Horizontal di Perairan Pesisir, Probolinggo, Jawa Timur. Jurnal Oseanografi, 2(3): 151 – 160
• Mujahid, T. Y., Wahab, A., Padhiar, S. H., Subhan, S. A., Baloch, M. N., and Pirzada, Z. A (2015) Isolation and Characterization of Hydrocarbon Degrading Bacteria from Petrol Contaminated Soil. Journal of Basic & Applied Sciences, 11(2015): 223 – 231
• Mulligan, C.N., Yong, R.N., and Gibbs, B.F (2001) Surfactant-enhanced remediation of contaminated soil: a review. Eng. Geol. 60, 371–380
• Olawale, O., Oyawale, F. A., Adepoju, T. F., Aikulolu, S., and Akinmoladun, A. I (2017) Optimisation of Diesel Polluted Soil using Response Surface Methodology. International Journal of Environmental Protection and Policy, 3(6): 194-202
• Palanisamy, N., Ramya, J., Kumar, S., Vasanthi, NS., Chandran, P., and Khan, S (2014) Diesel Biodegradation Capacities of Indigenous Bacterial Species Isolated from Diesel Contaminated Soil. Journal of Environmental Health Science & Engineering, 12(142): 1 – 8
• Patil, T. D., Pawar, S., Kamble, P. N., and Thakare, S. V (2012) Bioremediation of Complex Hydrocarbons using Microbial Consortium Isolated from Diesel Oil Polluted Soil. Der Chemica Sinica, 3(4): 953 – 958
• Pranowo, P. P., and Titah H. S (2016) Isolation and Screening of Diesel-Degrading Bacteria from the Diesel Contaminated Seawater at Kenjeran Beach, Surabaya. Environment Asia, 9(2): 165 – 169
• Prathyusha, K., Jagan Mohan, YSYV., Sridevi, S., and Sandeep, B.V (2016) Isolation and Characterization of Petroleum Hydrocarbon Degrading Indigenous Bacteria from Contaminated Sites of Visakhapatnam. International Journal of Advanced Research, 4(3): 357 – 362
• Ramasamy, S., Arumugam, A., and Chandran, P (2017) Optimization of Enterobacter cloacae (KU923381) for Diesel Oil Degradation using Response Surface Methodology (RSM). Journal of Microbiology, 55(2): 104–111
• Sathishkumar M., Binupriya A.R., Baik S.H, and Yun S.E (2008) Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium isolated from hydrocarbon contaminated areas. Clean-Soil Air Water, 36(1):92–96.
• Surendran, P. K., Iyer, K. M., and Gopakumar, K (1983) Salt Tolerant of Bactria Isolated from Tropical Marine Fish and Prawn. Fishery Technology, 20: 105 – 110
• Titah, H. S., Pratikno, H., Atiek Moesriati, A., Putera, R. I., and Imron, M.F (2017) Identification of Diesel Resistant Bacteria that Isolated from Ship Dismantling Area in Madura Coastal. Proceedings of International Conference : Problem, Solution and Development of Coastal and Delta Areas Semarang, Indonesia – September 26th, 2017, Paper No. C-06
• Whang L.M., Liu P.WG, Ma C.C, and Cheng S-S (2009) Application of rhamnolipid and surfactin for enhanced diesel biodegradation-Effects of pH and ammonium addition. J Hazard Mater, 164(2-3):1045–1050.
• Yuan, C., Li, C., Wang, Y., and Cui, Q (2015) Study on the Salinity Domestication and Mechanism of Nitrite Nitrogen Removal of Acinetobacter lwoffii. Advance Materials Research, 1092-1093: 641 – 644
• Zhang, Z., Gai, L., Hou, Z., Yang, C., Ma, C., Wang, Z., Sun, B., He, X., Tang, H., and Xu, P (2010) Characterization and biotechnological potential of petroleum-degrading bacteria isolated from oil-contaminated soils. Bioresour. Technol. 101, 8452–8456.