Research Article
BibTex RIS Cite

Effect of Different Nitrogen Sources on the Growth and Lipid Accumulation of Chlorella variabilis

Year 2018, Volume: 12 Issue: 2, 38 - 40, 25.09.2018

Abstract

Nitrogen is one of the major elements required for growth and other physiological activities of microalgae. Microalgae can use different forms of nitrogen such as nitrate, nitrite, ammonium, and urea. Although the type of nitrogen used by microalgae depends on the species, microalgae usually prefer ammonia as a nitrogen source and the usual order of preference is ammonium, urea, nitrate, and nitrite. However, types of nitrogen sources and their concentrations affect the growth of microalgae cultures and their biochemical structures. It has been reported in many studies that microalgae accumulate more lipids under nitrogen starvation growth conditions. Therefore,growth and lipid accumulation behavior of microalgae Chlorella variabilisin growth medium (BG11) containing different types of nitrogen source [sodium nitrate (NaNO3), ammonium chloride (NH4Cl) and urea(CH4N2O)] was investigated in this work. Maximum cell concentration (1.59 g/L) and growth rate (0.054/h) were obtained in NH4Cl containing photobioreactor, whereas highest lipid content (16.4%) and productivity (4.21 mg/L.day) was obtained in CH4N2O containing photobioreactor. Compared to the other nitrogen sources, NaNO3 did not significantly improve the growth rate and the lipid productivity. According to the results, microalgae can be considered as a raw material for biodiesel production applications in the future.

References

  • Siaut M, Heijde M, Mangogna M, Montsant A, Coesel S, Allen A, Bowler C. 2007. Molecular toolbox for studying diatom biology in Phaeodactylum tricornutum. Gene,406(1), 23-35.
  • Gökpınar Ş. 1991. Effect of Change of Temperature on Inorganic Nitrogen Assimilation of Five Important Sea Flagellat in Aquaculture, Doktora Tezi, Dokuz Eylül Üniversitesi, Deniz Bilimleri ve Teknolojisi Enstitüsü, İzmir, , 20260.
  • Xu N, Zhang X, Fan X, Han L, Zeng C. 2001. Effects of Nitrogen Source and Concentration on Growth Rate and Fatty Acid Composition of Ellipsoidion sp.(Eustigmatophyta), Journal of Applied Phycology, 13(6), 463-469.
  • Shifrin NS, Sallie WC. 1981. Phytoplankton Lipits: Interspecific Differences and Effects of Nitrate, Silicate and Light‐Dark Cycles, Journal of phycology, 17(4), 374-384.
  • Sukenik C. Y. A., Berner T. 1989 Regulation of Fatty Acid Composition by Irradiance Level in the Eustigmatophyte Nannochloropsis sp, Journal of Phycology, 25(4) 686- 692.
  • Grobbelaar JU. 2004. Algal Nutrition‒Mineral Nutrition, Handbook of microalgal culture: biotechnology and applied phycology, 95-115.
  • Bligh EG, & Dyer WJ, 1959. A rapid method of total lipit extraction and purification. Canadian journal of biochemistry and physiology, 37(8), 911-917.
  • Sharma AK, Sahoo PK, Singhal S. 2015. Influence of different nitrogen and organic carbon sources on microalgae growth and lipid production Journal of Pharmacy and Biological Sciences 10, 48-53
  • Arumugam M, Agarwal A, Arya MC, Ahmed Z. 2013. Influence of nitrogen sources on biomass productivity of microalgae Scenedesmus bijugatus, Bioresour ceTechnology, 131, 246–249.
  • Sweta MS, Sunny SS, MA, Rasheed PLS, Hasan RSZ. 2017. Effect of Various Nitrogen Sources on Microalgal Growth and Lipid Content in Chlorella pyrenoidosa NCIM 2738 and ANK-1. Int.J.Curr.Microbiol.App.Sci. 6(8): 3099-3108.
  • Qiang Lin, and Junda Lin, Effects of nitrogen source and concentration on biomass and oil production of a Scenedesmus rubescens like microalga, , Bioresour Technol 102(2), 2011, 1615-1621
Year 2018, Volume: 12 Issue: 2, 38 - 40, 25.09.2018

Abstract

References

  • Siaut M, Heijde M, Mangogna M, Montsant A, Coesel S, Allen A, Bowler C. 2007. Molecular toolbox for studying diatom biology in Phaeodactylum tricornutum. Gene,406(1), 23-35.
  • Gökpınar Ş. 1991. Effect of Change of Temperature on Inorganic Nitrogen Assimilation of Five Important Sea Flagellat in Aquaculture, Doktora Tezi, Dokuz Eylül Üniversitesi, Deniz Bilimleri ve Teknolojisi Enstitüsü, İzmir, , 20260.
  • Xu N, Zhang X, Fan X, Han L, Zeng C. 2001. Effects of Nitrogen Source and Concentration on Growth Rate and Fatty Acid Composition of Ellipsoidion sp.(Eustigmatophyta), Journal of Applied Phycology, 13(6), 463-469.
  • Shifrin NS, Sallie WC. 1981. Phytoplankton Lipits: Interspecific Differences and Effects of Nitrate, Silicate and Light‐Dark Cycles, Journal of phycology, 17(4), 374-384.
  • Sukenik C. Y. A., Berner T. 1989 Regulation of Fatty Acid Composition by Irradiance Level in the Eustigmatophyte Nannochloropsis sp, Journal of Phycology, 25(4) 686- 692.
  • Grobbelaar JU. 2004. Algal Nutrition‒Mineral Nutrition, Handbook of microalgal culture: biotechnology and applied phycology, 95-115.
  • Bligh EG, & Dyer WJ, 1959. A rapid method of total lipit extraction and purification. Canadian journal of biochemistry and physiology, 37(8), 911-917.
  • Sharma AK, Sahoo PK, Singhal S. 2015. Influence of different nitrogen and organic carbon sources on microalgae growth and lipid production Journal of Pharmacy and Biological Sciences 10, 48-53
  • Arumugam M, Agarwal A, Arya MC, Ahmed Z. 2013. Influence of nitrogen sources on biomass productivity of microalgae Scenedesmus bijugatus, Bioresour ceTechnology, 131, 246–249.
  • Sweta MS, Sunny SS, MA, Rasheed PLS, Hasan RSZ. 2017. Effect of Various Nitrogen Sources on Microalgal Growth and Lipid Content in Chlorella pyrenoidosa NCIM 2738 and ANK-1. Int.J.Curr.Microbiol.App.Sci. 6(8): 3099-3108.
  • Qiang Lin, and Junda Lin, Effects of nitrogen source and concentration on biomass and oil production of a Scenedesmus rubescens like microalga, , Bioresour Technol 102(2), 2011, 1615-1621
There are 11 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Togayhan Kutluk

Necla Altın This is me

Başar Uyar This is me

Nurcan Kapucu This is me

Publication Date September 25, 2018
Published in Issue Year 2018 Volume: 12 Issue: 2

Cite

APA Kutluk, T., Altın, N., Uyar, B., Kapucu, N. (2018). Effect of Different Nitrogen Sources on the Growth and Lipid Accumulation of Chlorella variabilis. Journal of Applied Biological Sciences, 12(2), 38-40.
AMA Kutluk T, Altın N, Uyar B, Kapucu N. Effect of Different Nitrogen Sources on the Growth and Lipid Accumulation of Chlorella variabilis. J.appl.biol.sci. September 2018;12(2):38-40.
Chicago Kutluk, Togayhan, Necla Altın, Başar Uyar, and Nurcan Kapucu. “Effect of Different Nitrogen Sources on the Growth and Lipid Accumulation of Chlorella Variabilis”. Journal of Applied Biological Sciences 12, no. 2 (September 2018): 38-40.
EndNote Kutluk T, Altın N, Uyar B, Kapucu N (September 1, 2018) Effect of Different Nitrogen Sources on the Growth and Lipid Accumulation of Chlorella variabilis. Journal of Applied Biological Sciences 12 2 38–40.
IEEE T. Kutluk, N. Altın, B. Uyar, and N. Kapucu, “Effect of Different Nitrogen Sources on the Growth and Lipid Accumulation of Chlorella variabilis”, J.appl.biol.sci., vol. 12, no. 2, pp. 38–40, 2018.
ISNAD Kutluk, Togayhan et al. “Effect of Different Nitrogen Sources on the Growth and Lipid Accumulation of Chlorella Variabilis”. Journal of Applied Biological Sciences 12/2 (September 2018), 38-40.
JAMA Kutluk T, Altın N, Uyar B, Kapucu N. Effect of Different Nitrogen Sources on the Growth and Lipid Accumulation of Chlorella variabilis. J.appl.biol.sci. 2018;12:38–40.
MLA Kutluk, Togayhan et al. “Effect of Different Nitrogen Sources on the Growth and Lipid Accumulation of Chlorella Variabilis”. Journal of Applied Biological Sciences, vol. 12, no. 2, 2018, pp. 38-40.
Vancouver Kutluk T, Altın N, Uyar B, Kapucu N. Effect of Different Nitrogen Sources on the Growth and Lipid Accumulation of Chlorella variabilis. J.appl.biol.sci. 2018;12(2):38-40.