Sağlıklı ve Diyabetli Sıçanlarda Beyin ve Pankreas Dokusundan İzole Edilen Eksozom -miRNA -9 ve -146 Profillerinin Karşılaştırılması

Yıl 2023, , 671 - 678, 31.12.2023

Sıdıka Genç , Ali Taghizadehghalehjoughi

https://doi.org/10.35229/jaes.1358883

Öz

Eksozom adı verilen küçük veziküllerin dokulardaki gen ekspresyonunu düzenlediği ve birçok hastalığın patogenezinde rol oynadığı bulunmuştur. Bu nedenle, bu çalışma ekzozomların diyabet ve buna bağlı mikroRNA (miRNA) değişimi üzerindeki etkilerini belirlemeyi amaçlamıştır. Bu amaçla intraperitoneal olarak nikotinamid (120 mg/kg) uygulandı (ip.) ve 15 dakika sonra Streptozotosin (50 mg / kg) ip uygulandı. Glikoz seviyesi 126 mg/dL ve üzeri olan sıçanlar Tip 2 diyabet olarak kabul edildi. 21 Günün sonunda şeker hastalarının ve sağlıklı grupların sıçanlarının (n:10) pankreas ve beyin dokularından eksozomlar elde edildi. Daha sonra her iki grubun biyokimyasal analizleri ve oksidatif stres parametreleri incelendi. Ayrıca miRNA değişiklikleri incelenmiş ve elde edilen sonuçlar istatistiksel olarak değerlendirilmiştir. Hem beyin hem de pankreas ekzozomlarında toplam oksidatif durum (TOS) ve Laktat dehidrojenaz (LDH) seviyelerinde anlamlı bir artış bulundu. Total antioksidan düzeyinin (TAC) kontrol grubuna göre azaldığı gözlendi (P<0.05). Gerçek Zamanlı PCR ile yapılan inceleme sonucunda hem beyin dokusunda hem de pankreas dokusunda miRNA -9 düzeylerinin önemli ölçüde arttığı ve miR-146 gen düzeylerinin önemli ölçüde aşağı regüle edildiği belirlendi (P<0.05).
Sonuç olarak, diyabetik sıçanların beyin ve pankreas dokusu eksozomlarında miRNA -9 ve -146 seviyelerinde önemli değişiklikler meydana geldi. Bu sonuçlar, diyabetik rat eksozomların miRNA düzeyinde değişikliğe neden olduğu ve bu değişikliğin nöroinflamasyonla ilişkili olabileceğini göstermektedir.

Anahtar Kelimeler

Diabetes, miR-9, miR21, LDH

Comparison of Brain and Pancreas Tissues Exosome derived -miRNA -9 and -146 levels in Healthy and Diabetes Rats

Öz

Small vesicles called exosomes have been found to regulate gene expression in tissues and play a role in the pathogenesis of many diseases. Therefore, this study aimed to determine the effects of exosomes on diabetes and related microRNA (miRNA) exchange. For this purpose, nicotinamide (120 mg/kg) was administered intraperitoneally (IP.), and Streptozotocin (50 mg/kg) ip was allocated 15 minutes later. Rats with 126 mg/dL and up glucose levels were accepted as Type 2 diabetes. At the end of 21 days, exosomes were obtained from the pancreas and brain tissues of rats (n:10) of diabetics and healthy groups. Then biochemical analyzes and oxidative stress parameters of both groups were examined. In addition, miRNA changes were discussed, and the results obtained were statistically evaluated. A significant increase was found in total oxidative status (TOS) and Lactate dehydrogenase (LDH) levels in both brain and pancreatic exosomes. It was observed that the total antioxidant level (TAC) decreased compared to the control group (P<0.05). As a result of the examination with Real-Time PCR, it was determined that the levels of miRNA -9 increased significantly in both brain tissue and pancreas tissue, and miR-146 gene levels were down-regulated considerably (P<0.05).
As a result, significant changes occurred in miRNA -9 and -146 levels in the brain and pancreatic tissue exosomes of diabetic rats. The results suggest that exosomes may have a role in miRNAs mediating neuroinflammation in the brain and pancreatic tissue.

Anahtar Kelimeler

miRNA-9, Diabetes, miRNA-146, LDH, TOS

Kaynakça

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