Comparison of Brain and Pancreas Tissues Exosome derived -miRNA -9 and -146 levels in Healthy and Diabetes Rats
Abstract
Small vesicles called exosomes have been found to regulate gene expression in tissues and play a role in the pathogenesis of many diseases. Therefore, this study aimed to determine the effects of exosomes on diabetes and related microRNA (miRNA) exchange. For this purpose, nicotinamide (120 mg/kg) was administered intraperitoneally (IP.), and Streptozotocin (50 mg/kg) ip was allocated 15 minutes later. Rats with 126 mg/dL and up glucose levels were accepted as Type 2 diabetes. At the end of 21 days, exosomes were obtained from the pancreas and brain tissues of rats (n:10) of diabetics and healthy groups. Then biochemical analyzes and oxidative stress parameters of both groups were examined. In addition, miRNA changes were discussed, and the results obtained were statistically evaluated. A significant increase was found in total oxidative status (TOS) and Lactate dehydrogenase (LDH) levels in both brain and pancreatic exosomes. It was observed that the total antioxidant level (TAC) decreased compared to the control group (P<0.05). As a result of the examination with Real-Time PCR, it was determined that the levels of miRNA -9 increased significantly in both brain tissue and pancreas tissue, and miR-146 gene levels were down-regulated considerably (P<0.05).
As a result, significant changes occurred in miRNA -9 and -146 levels in the brain and pancreatic tissue exosomes of diabetic rats. The results suggest that exosomes may have a role in miRNAs mediating neuroinflammation in the brain and pancreatic tissue.
Keywords
References
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