Determination of Antimicrobial Activity and MIC Value of Tannic Acid Against Four Different Fish Pathogens

Year 2024, , 582 - 589, 31.12.2024

Zeynep Dengiz Balta , Fikri Balta

https://doi.org/10.35229/jaes.1573899

Abstract

In this study, the antimicrobial susceptibility of Silvafeed TSP (tannic acid) obtained from chestnut tree using natural extraction method and used as a feed sweetener in aquaculture against some fish pathogens was investigated. Antimicrobial susceptibility of different concentrations of tannic acid was determined against fish pathogens that cause significant economic losses by bacteria such as Aeromonas hydrophila, Pseudomonas fluorescens, Yersinia ruckeri and Vibrio anguillarum. According to the antimicrobial test results, it was determined that all isolates to tested antibiotics were resistant to ampicillin, however, all isolates were susceptible to other antibiotics except for P. fluorescence isolate. It was determined that the P. fluorescence isolate was resistant to other antibiotics except gentamicin and doxycycline. According to the antimicrobial susceptibility test results of tannic acid against E. coli and four different fish pathogens, P. fluorescens and V. anguillarum were found to be sensitive above 125 µg/ml concentrations. In the same study, E. coli, A. hydrophila, and Y. ruckeri were found to be sensitive above 250 µg/ml concentrations. It was determined that P. fluorescens and V. anguillarum were resistant to tannic acid concentrations below 62.5 µg/ml. Moreover, E. coli, A. hydrophila, and Y. ruckeri were resistant to tannic acid concentrations below 125 µg/ml.

Keywords

Antimicrobial susceptibility, aquaculture, tannic acid, treatment

Supporting Institution

yok

Thanks

Tannic asit Profeed Food and Chemical Substances Industry and Trade Ltd. Şti. ücretsiz olarak sağlanmıştır. İlgili kuruma teşekkür ederiz.

Tannik Asidin Dört Farklı Balık Patojenine Karşı Antimikrobiyal Aktivitesinin ve MİK Değerinin Belirlenmesi

Abstract

Bu çalışmada, kestane ağacından doğal ekstraksiyon yöntemi ile elde edilen ve su ürünleri yetiştiriciliğinde yem tatlandırıcı olarak kullanılan Silvafeed TSP'nin (tannik asit) bazı balık patojenlerine karşı antimikrobiyal duyarlılığı araştırılmıştır. Aeromonas hydrophila, Pseudomonas fluorescens, Yersinia ruckeri ve Vibrio anguillarum gibi bakteriler tarafından önemli ekonomik kayıplara neden olan balık patojenlerine karşı farklı konsantrasyonlarda tannik asidin antimikrobiyal duyarlılığı belirlendi. Antimikrobiyal test sonuçlarına göre, test edilen antibiyotiklere karşı izolatların tamamının ampisiline dirençli olduğu, ancak P. fluoresans izolatı hariç diğer antibiyotiklere duyarlı olduğu belirlendi. P. fluorescence izolatının gentamisin ve doksisiklin hariç diğer antibiyotiklere dirençli olduğu belirlendi. Tannik asidin E. coli ve dört farklı balık patojeni olan P. fluorescens ve V. anguillarum'a karşı antimikrobiyal duyarlılık test sonuçlarına göre, 125 µg/ml konsantrasyonun üstünde duyarlı olduğu bulundu. Aynı çalışmada E. coli, A. hydrophila ve Y. ruckeri'nin 250 µg/ml konsantrasyonun üzerinde duyarlı olduğu bulunmuştur. P. fluorescens ve V. anguillarum'un 62,5 µg/ml'nin altındaki tannik asit konsantrasyonlarına dirençli olduğu belirlendi. Ayrıca, E. coli, A. hydrophila ve Y. ruckeri'nin 125 µg/ml'nin altındaki tannik asit konsantrasyonlarına dirençli olduğu belirlendi.

Keywords

Antimikrobiyal duyarlılık, su ürünleri yetiştiriciliği, tannik asit, tedavi

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