Comparative Assessment of Faecal Microbiota in Healthy and Epileptic Dogs

Nilgün Aksoy; Şeyda Yaman; Alper Çiftci

doi:10.35229/jaes.1598473

EN TR

Comparative Assessment of Faecal Microbiota in Healthy and Epileptic Dogs

Abstract

Idiopathic epilepsy is a neurological condition affecting dogs and cats characterized by repeated seizures for which no cause other than a hypothesized genetic predisposition can be identified. The aim of this study was to comparatively evaluate the content of mesophilic aerobic bacteria, coliform bacteria, Escherichia coli, Enterococcus spp., Staphylococcus aureus and Lactobacillus spp. in the faecal flora of healthy dogs and dogs diagnosed with epilepsy. Feces of 10 healthy dogs and 10 dogs diagnosed with epilepsy were used as study material. Selective isolation and counts were performed with inoculating the faecal samples were on Tryptic Soy Agar for mesophilic aerobic bacteria, MacConkey Agar for coliform bacteria, Eosin Methylene Blue Agar for E. coli, Bile Esculin Agar for Enterococcus spp., Mannitol Salt Agar for S. aureus and De Man, Rogosa and Sharpe Agar for Lactobacillus spp. The numbers of mesophilic aerobic bacteria, coliform bacteria, E. coli, Enterococcus spp., S. aureus and Lactobacillus spp. were 6.6x106, 2.6x105, 1.4x105, 1.6x105, 3.2x104 and 4.1 x 104 cfu/mg in the faecal flora of healthy dogs; and 9.0x106, 5.0x105, 2.8x105, 6.4x105, 6.3x104, and 1.2x104 cfu/mg in dogs diagnosed with epilepsy, respectively. As a result, there was a decrease in the number of Lactobacillus spp. in the faecal flora of dogs with epilepsy compared to healthy dogs, while the number of other bacteria increased. It was concluded that adding probiotic bacteria to the diet of epileptic dogs could help regulate the gut microbiota and reduce the severity and frequency of seizures.

Keywords

dog , faecal flora , idiopathic epilepsy

Sağlıklı ve Epileptik Köpeklerde Fekal Mikrobiyotanın Karşılaştırmalı Değerlendirmesi

Öz

İdiyopatik epilepsi, hipotez edilen genetik yatkınlıktan başka bir nedenin tanımlanamadığı, tekrarlayan nöbetlerle karakterize, köpekleri ve kedileri etkileyen nörolojik bir durumdur. Bu çalışma sağlıklı köpeklerin ve epilepsi tanısı almış köpeklerin fekal florasındaki mezofilik aerobik bakteri, koliform bakteri, Escherichia coli, Enterococcus spp., Staphylococcus aureus ve Lactobacillus spp. içeriğini karşılaştırmalı olarak değerlendirmek amacıyla gerçekleştirildi. Çalışma materyali olarak 10 sağlıklı ve 10 epilepsi tanısı almış köpeğin dışkısı kullanıldı. Seçici izolasyon ve sayımlar, dışkı örneklerinin mezofilik aerobik bakteriler için Tryptic Soy Agar, koliform bakteriler için MacConkey Agar, E. coli için Eosin Metilen Mavisi Agar, Enterococcus spp. için Bile Eskülin Agar, S. aureus için Mannitol Salt Agar ve Lactobacillus spp. için De Man, Rogosa ve Sharpe Agar'a ekilmesiyle gerçekleştirildi. Sağlıklı köpeklerin dışkı florasında mezofilik aerobik bakteri, koliform bakteri, E. coli, Enterococcus spp., S. aureus ve Lactobacillus spp. sayıları sırasıyla 6,6x106, 2,6x105, 1,4x105, 1,6x105, 3,2x104 ve 4,1 x 104 kob/mg ve epilepsi tanısı konulan köpeklerde sırasıyla 9,0x106, 5,0x105, 2,8x105, 6,4x105, 6,3x104 ve 1,2x104 kob /mg olarak bulundu. Sonuç olarak, epilepsili köpeklerin dışkı florasında Lactobacillus spp. sayısında sağlıklı köpeklere kıyasla bir azalma görülürken, diğer bakterilerin sayısında artış görüldü. Epileptik köpeklerin diyetine probiyotik bakteri eklenmesinin bağırsak mikrobiyotasını düzenlemeye ve nöbetlerin şiddetini ve sıklığını azaltmaya yardımcı olabileceği kanısına varıldı.

Anahtar Kelimeler

dışkı florası , idiyopatik epilepsi , köpek

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Details

Primary Language

English

Subjects

Veterinary Microbiology

Journal Section

Research Article

Authors

Nilgün Aksoy
0009-0008-0988-1867
Türkiye

Şeyda Yaman
0000-0003-2175-6109
Türkiye

Alper Çiftci ^*
0000-0001-8370-8677
Türkiye

Early Pub Date

January 28, 2025

Publication Date

January 31, 2025

Submission Date

December 9, 2024

Acceptance Date

January 25, 2025

Published in Issue

Year 2025 Volume: 10 Number: 1

DOI

https://doi.org/10.35229/jaes.1598473

IZ

https://izlik.org/JA55UN67ZZ

APA

Aksoy, N., Yaman, Ş., & Çiftci, A. (2025). Comparative Assessment of Faecal Microbiota in Healthy and Epileptic Dogs. Journal of Anatolian Environmental and Animal Sciences, 10(1), 79-86. https://doi.org/10.35229/jaes.1598473

EBSCOHost Scilit CABI

JAES/AAS-Journal of Anatolian Environmental and Animal Sciences/Anatolian Academic Sciences&Anadolu Çevre ve Hayvancılık Dergisi/Anadolu Akademik Bilimler-AÇEH/AAS

Comparative Assessment of Faecal Microbiota in Healthy and Epileptic Dogs

Abstract

Keywords

Sağlıklı ve Epileptik Köpeklerde Fekal Mikrobiyotanın Karşılaştırmalı Değerlendirmesi

Öz

Anahtar Kelimeler

References

Details

Primary Language

Subjects

Journal Section

Authors

Early Pub Date

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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