Developing Lucilia sericata Larva on the Natural Liver or Liver Agar
Abstract
Foods with high protein and organic content are effective in the maturation of sperm and eggs of Calliphoridae and mating. In Lucilia sericata fly colony continuity, liver food in the procedure in terms of both chemical properties and protein and moisture content is a suitable food substrate for females to lay their eggs. However, liver or any other animal tissue can cause contamination due to decomposition during larval development of the eggs. Many sterilised artificial diets have been developed for controlled mass rearing. In this study, natural diet liver and liver-agar artificial diets containing natural diet were preferred after spawning. In particular, the effects of these diets on the following characteristics of L sericata were analysed: 1) growth and development time 2) larval stage length and weight 3) pupal and adult length 4) morphological appearance of larvae, pupae and adults. The experiment was conducted in two replicate experiments with six study groups in each. In general, rearing on liver-based media was similar, but growth and development on sterile liver-agar media was more efficient than on liver and no contamination occurred. There was a significant difference (p<0.05) between measurements of first instar larval length, first instar larval weight, second instar larval weight, third instar larval length, third instar larval weight, pupal length and adult length of L. sericata larvae fed on liver and liver-agar. However, there was no significant difference between the second instar larval length measurements of L. sericata larvae fed with liver-agar and liver (p>0.05).
Keywords
Artificial substrate , growth and development , larval feeding , Lucilia sericata
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