Isolation and Enzyme Activity Profiling of Phytohormone-Degrading Bacteria from Soil, Rhizosphere, and Tissues within the Camellia sinensis Ecosystem

Ayşenur Eminoğlu; Abdülkadir Yılmaz

doi:10.35229/jaes.1831268

TR EN

Camellia sinensis Ekosistemindeki Toprak, Rizosfer ve Bitki Dokularından Fitohormon Parçalayıcı Bakterilerin İzolasyonu ve Enzim Aktivitesi Profilinin Belirlenmesi

Öz

Bu çalışmada, çay ekosisteminde yer alan bakteri topluluklarının fitohormon parçalayıcı potansiyeli araştırılmıştır. Toprak, rizosfer, ve tohum gibi farklı mikrohabitatlardan toplam 79 bakteri izole edilmiş ve bu izolatlarda ACC, IAA ve salisilik asit (SA) degradasyon aktiviteleri taranmıştır. Aktivite gösteren seçilmiş izolatların 16S rRNA gen dizilemesi yapılmış ve filogenetik analizle taksonomik konumları belirlenmiştir. Elde edilen diziler referans türlerle anlamlı kümelenmeler göstermiş ve izolatların ağırlıklı olarak Burkholderia, Acinetobacter ve Pseudomonas cinslerine ait olduğu gösterilmiştir. İzolatlar arasında en az tespit edilen aktivitenin SA degradasyonu olması belirli ekolojik veya tarımsal koşulların SA parçalayıcı mikroorganizmaları destekleyebileceğini ortaya koymuştur. ACC deaminaz (ACCd) aktivitesi 0–2.58 µmol mg⁻¹ h⁻¹ aralığında bulunmuş, en yüksek değer Burkholderia sp. 6R1 izolatında ölçülmüş ve bu değer Burkholderia türleri için bildirilen aralıkla karşılaştırılabilir düzeyde olmuştur. IAA degradasyonunun üç farklı cinste görülmesi, bu özelliğin çeşitli bakteri grupları arasında yaygın olduğunu göstermektedir. Çay mikrobiyomunun fitohormon dengesini etkileyen bu fonksiyonel özellikleri, bitki gelişimi ve stres yanıtlarının düzenlenmesinde rol oynayabilir. Sonuç olarak bu çalışma, çay ekosisteminden izole edilen bakterilerin temel fitohormonları parçalayıcı kapasiteye sahip olduğunu ve bu özelliklerin sürdürülebilir çay yetiştiriciliğinde biyolojik düzenleyici potansiyeli taşıdığını ortaya koymaktadır.

Anahtar Kelimeler

Camellia sinensis , fitohormon degradasyonu , ACCd , IAA , salisilik asit

Isolation and Enzyme Activity Profiling of Phytohormone-Degrading Bacteria from Soil, Rhizosphere, and Tissues within the Camellia sinensis Ecosystem

Abstract

This study investigated the phytohormone-degrading potential of bacterial communities within the tea ecosystem. A total of 79 bacterial isolates were obtained from various microhabitats, including soil, rhizosphere, roots, and seeds, and screened for their ability to degrade ACC, IAA, and salicylic acid (SA). Selected active isolates were subjected to 16S rRNA gene sequencing, and their taxonomic affiliations were determined through phylogenetic analysis. The sequences clustered with reference species with significant bootstrap support, revealing that the isolates predominantly belonged to the genera Burkholderia, Acinetobacter, and Pseudomonas. Among the isolates, SA degradation activity was the least frequently observed, suggesting that specific ecological or agricultural conditions may influence the presence of SA-degrading microorganisms. ACC deaminase (ACCd) activity ranged from 0 to 2.58 µmol mg⁻¹ h⁻¹, with the highest value recorded in Burkholderia sp. isolate 6R1, consistent with previously reported ranges for Burkholderia species. The detection of IAA degradation across three genera indicates that this trait is widespread among diverse bacterial groups. These functional properties, which influence phytohormone balance in the tea microbiome, may play a role in modulating plant growth and stress responses. In conclusion, the findings demonstrate that bacteria isolated from the tea ecosystem possess the capacity to degrade key phytohormones, highlighting their potential as biological regulators for sustainable tea cultivation.

Keywords

Camellia sinensis , phytohormone degradation , ACCd , IAA , salicylic acid.

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Details

Primary Language

English

Subjects

Genetics (Other)

Journal Section

Research Article

Authors

Ayşenur Eminoğlu ^*
0000-0003-1693-6332
Türkiye

Abdülkadir Yılmaz This is me
0009-0005-0382-4333
Türkiye

Publication Date

February 28, 2026

Submission Date

November 27, 2025

Acceptance Date

January 25, 2026

Published in Issue

Year 2026 Number: 2026

DOI

https://doi.org/10.35229/jaes.1831268

IZ

https://izlik.org/JA35CA79NE

APA

Eminoğlu, A., & Yılmaz, A. (2026). Isolation and Enzyme Activity Profiling of Phytohormone-Degrading Bacteria from Soil, Rhizosphere, and Tissues within the Camellia sinensis Ecosystem. Journal of Anatolian Environmental and Animal Sciences, 2026. https://doi.org/10.35229/jaes.1831268

EBSCOHost Scilit CABI

JAES/AAS-Journal of Anatolian Environmental and Animal Sciences/Anatolian Academic Sciences&Anadolu Çevre ve Hayvancılık Dergisi/Anadolu Akademik Bilimler-AÇEH/AAS

Camellia sinensis Ekosistemindeki Toprak, Rizosfer ve Bitki Dokularından Fitohormon Parçalayıcı Bakterilerin İzolasyonu ve Enzim Aktivitesi Profilinin Belirlenmesi

Öz

Anahtar Kelimeler

Isolation and Enzyme Activity Profiling of Phytohormone-Degrading Bacteria from Soil, Rhizosphere, and Tissues within the Camellia sinensis Ecosystem

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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