Isolation and Enzyme Activity Profiling of Phytohormone-Degrading Bacteria from Soil, Rhizosphere, and Tissues within the Camellia sinensis Ecosystem

Ayşenur Eminoğlu; Abdülkadir Yılmaz

doi:10.35229/jaes.1831268

TR EN

Camellia sinensis Ekosistemindeki Toprak, Rizosfer ve Bitki Dokularından Fitohormon Parçalayıcı Bakterilerin İzolasyonu ve Enzim Aktivitesi Profilinin Belirlenmesi

Öz

Bu çalışmada, çay ekosisteminde yer alan bakteri topluluklarının fitohormon parçalayıcı potansiyeli araştırılmıştır. Toprak, rizosfer, ve tohum gibi farklı mikrohabitatlardan toplam 79 bakteri izole edilmiş ve bu izolatlarda ACC, IAA ve salisilik asit (SA) degradasyon aktiviteleri taranmıştır. Aktivite gösteren seçilmiş izolatların 16S rRNA gen dizilemesi yapılmış ve filogenetik analizle taksonomik konumları belirlenmiştir. Elde edilen diziler referans türlerle anlamlı kümelenmeler göstermiş ve izolatların ağırlıklı olarak Burkholderia, Acinetobacter ve Pseudomonas cinslerine ait olduğu gösterilmiştir. İzolatlar arasında en az tespit edilen aktivitenin SA degradasyonu olması belirli ekolojik veya tarımsal koşulların SA parçalayıcı mikroorganizmaları destekleyebileceğini ortaya koymuştur. ACC deaminaz (ACCd) aktivitesi 0–2.58 µmol mg⁻¹ h⁻¹ aralığında bulunmuş, en yüksek değer Burkholderia sp. 6R1 izolatında ölçülmüş ve bu değer Burkholderia türleri için bildirilen aralıkla karşılaştırılabilir düzeyde olmuştur. IAA degradasyonunun üç farklı cinste görülmesi, bu özelliğin çeşitli bakteri grupları arasında yaygın olduğunu göstermektedir. Çay mikrobiyomunun fitohormon dengesini etkileyen bu fonksiyonel özellikleri, bitki gelişimi ve stres yanıtlarının düzenlenmesinde rol oynayabilir. Sonuç olarak bu çalışma, çay ekosisteminden izole edilen bakterilerin temel fitohormonları parçalayıcı kapasiteye sahip olduğunu ve bu özelliklerin sürdürülebilir çay yetiştiriciliğinde biyolojik düzenleyici potansiyeli taşıdığını ortaya koymaktadır.

Anahtar Kelimeler

Camellia sinensis , fitohormon degradasyonu , ACCd , IAA , salisilik asit

Isolation and Enzyme Activity Profiling of Phytohormone-Degrading Bacteria from Soil, Rhizosphere, and Tissues within the Camellia sinensis Ecosystem

Abstract

This study investigated the phytohormone-degrading potential of bacterial communities within the tea ecosystem. A total of 79 bacterial isolates were obtained from various microhabitats, including soil, rhizosphere, roots, and seeds, and screened for their ability to degrade ACC, IAA, and salicylic acid (SA). Selected active isolates were subjected to 16S rRNA gene sequencing, and their taxonomic affiliations were determined through phylogenetic analysis. The sequences clustered with reference species with significant bootstrap support, revealing that the isolates predominantly belonged to the genera Burkholderia, Acinetobacter, and Pseudomonas. Among the isolates, SA degradation activity was the least frequently observed, suggesting that specific ecological or agricultural conditions may influence the presence of SA-degrading microorganisms. ACC deaminase (ACCd) activity ranged from 0 to 2.58 µmol mg⁻¹ h⁻¹, with the highest value recorded in Burkholderia sp. isolate 6R1, consistent with previously reported ranges for Burkholderia species. The detection of IAA degradation across three genera indicates that this trait is widespread among diverse bacterial groups. These functional properties, which influence phytohormone balance in the tea microbiome, may play a role in modulating plant growth and stress responses. In conclusion, the findings demonstrate that bacteria isolated from the tea ecosystem possess the capacity to degrade key phytohormones, highlighting their potential as biological regulators for sustainable tea cultivation.

Keywords

Camellia sinensis , phytohormone degradation , ACCd , IAA , salicylic acid.

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Genetik (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Ayşenur Eminoğlu ^*
0000-0003-1693-6332
Türkiye

Abdülkadir Yılmaz Bu kişi benim
0009-0005-0382-4333
Türkiye

Yayımlanma Tarihi

28 Şubat 2026

Gönderilme Tarihi

27 Kasım 2025

Kabul Tarihi

25 Ocak 2026

Yayımlandığı Sayı

Yıl 2026 Sayı: 2026

DOI

https://doi.org/10.35229/jaes.1831268

IZ

https://izlik.org/JA35CA79NE

APA

Eminoğlu, A., & Yılmaz, A. (2026). Isolation and Enzyme Activity Profiling of Phytohormone-Degrading Bacteria from Soil, Rhizosphere, and Tissues within the Camellia sinensis Ecosystem. Journal of Anatolian Environmental and Animal Sciences, 2026. https://doi.org/10.35229/jaes.1831268

JAES, 2016- 11.yıl

Camellia sinensis Ekosistemindeki Toprak, Rizosfer ve Bitki Dokularından Fitohormon Parçalayıcı Bakterilerin İzolasyonu ve Enzim Aktivitesi Profilinin Belirlenmesi

Öz

Anahtar Kelimeler

Isolation and Enzyme Activity Profiling of Phytohormone-Degrading Bacteria from Soil, Rhizosphere, and Tissues within the Camellia sinensis Ecosystem

Abstract

Keywords

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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