Doğu Karadeniz’in Önemli Bir Tarım Zararlısı Orosanga japonica (Melichar, 1898) (Homoptera: Ricaniidae)’ya Karşı Bitkisel Asetilkolinesteraz İnhibitörlerinin Değerlendirilmesi Değerlendirilmesi

Demet Kızıl

doi:10.35229/jaes.1862726

EN TR

Evaluation of Plant-Based Acetylcholinesterase Inhibitors Against Orosanga japonica (Melichar, 1898) (Homoptera: Ricaniidae), an Important Agricultural Pest in the Eastern Black Sea Regionl Pest in the Eastern Black Sea Region

Abstract

Acetylcholinesterase (AChE) is an important enzyme that plays a critical role in nerve transmission and is the primary target of many insecticides. Orosanga japonica is an invasive pest species that causes serious economic losses in agricultural production. Due to the environmental effects of synthetic insecticides, the development of natural and environmentally friendly alternatives has become important. In this study, the inhibitory effects of aqueous extracts obtained from sumac (Rhus coriaria L.), laurel (Laurus nobilis L.), Goji berry (Lycium barbarum L.), cornelian cherry (Cornus mas L.), and aronia (Aronia melanocarpa (Michx.) Elliott) were investigated for their inhibitory effects on AChE enzymes isolated from the nymph and adult stages of O. japonica under in vitro conditions. Among the positive controls, edrophonium chloride exhibited the strongest inhibitory effect, with IC₅₀ values of 0.76 ± 0.1 and 2.6 ± 0.5 µM for nymph and adult AChE, respectively. Among plant extracts, sumac fruit stood out as the most effective natural extract, exhibiting the lowest IC₅₀ values for both nymph (48.3 ± 8.2 µg dry matter/mL) and adult (66.1 ± 11.5 µg dry matter/mL) stages. In general, it was determined that AChE obtained from adult individuals showed lower sensitivity to inhibitors compared to the nymph stage. The findings indicate that all plant aqueous extracts studied exhibited inhibitory effects on O. japonica AChE at different levels and that sumac fruit extract, in particular, stands out as a promising candidate for the development of natural and environmentally friendly bioinsecticides due to its lower IC₅₀ values compared to other extracts.

Keywords

Acetylcholinesterase, Biopesticides, İnhibition, Orosanga japonica, Pest Management

Doğu Karadeniz’in Önemli Bir Tarım Zararlısı Orosanga japonica (Melichar, 1898) (Homoptera: Ricaniidae)’ya Karşı Bitkisel Asetilkolinesteraz İnhibitörlerinin Değerlendirilmesi Değerlendirilmesi

Abstract

Asetilkolinesteraz (AChE), sinir iletiminde kritik rol oynayan ve birçok insektisitin temel hedefi olan önemli bir enzimdir. Orosanga japonica, tarımsal üretimde ciddi ekonomik kayıplara yol açan istilacı bir zararlı tür olup, sentetik insektisitlerin çevresel etkileri nedeniyle doğal ve çevre dostu alternatiflerin geliştirilmesi önem kazanmaktadır. Bu çalışmada, sumak (Rhus coriaria L.), defne (Laurus nobilis L.), kurt üzümü (Lycium barbarum L.), kızılcık (Cornus mas L.) ve aronya (Aronia melanocarpa (Michx.) Elliott) bitkilerinden elde edilen sulu ekstraktların, O. japonica’nın nimf ve ergin dönemlerinden izole edilen AChE enzimleri üzerindeki inhibitör etkileri in vitro koşullarda araştırılmıştır. Pozitif kontrollerden edrofonyum klorit, nimf ve ergin AChE için sırasıyla 0,76 ± 0,1 ve 2,6 ± 0,5 µM IC₅₀ değerleriyle en güçlü inhibitör etkiyi göstermiştir. Bitkisel ekstraktlar arasında ise sumak meyvesi, nimf (48,3 ± 8,2 µg kuru madde/mL) ve ergin (66,1 ± 11,5 µg kuru madde/mL) dönemlerde en düşük IC₅₀ değerlerini sergileyerek en etkili doğal ekstrakt olarak öne çıkmıştır. Genel olarak, ergin bireylerden elde edilen AChE’nin nimf dönemine kıyasla inhibitörlere karşı daha düşük duyarlılık gösterdiği belirlenmiştir. Bulgular, incelenen tüm bitkisel sulu ekstraktların O. japonica AChE’si üzerinde farklı düzeylerde inhibitör etki gösterdiğini ve özellikle sumak meyvesi ekstraktının diğer ekstraktlara kıyasla daha düşük IC₅₀ değerleri sergilemesi nedeniyle doğal kökenli ve çevre dostu biyoinsektisitlerin geliştirilmesine yönelik umut verici bir aday olarak öne çıktığını göstermektedir

Keywords

Asetilkolinesteraz, Biyopestisitler, Haşere Kontrolü, İnhibisyon, Orosanga japonica

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Turkish

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Entomology, Conservation and Biodiversity

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Research Article

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Demet Kızıl ^*
0000-0002-2346-7514
Türkiye

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March 17, 2026

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January 13, 2026

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February 25, 2026

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https://doi.org/10.35229/jaes.1862726

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https://izlik.org/JA37MP68LW

APA

Kızıl, D. (2026). Doğu Karadeniz’in Önemli Bir Tarım Zararlısı Orosanga japonica (Melichar, 1898) (Homoptera: Ricaniidae)’ya Karşı Bitkisel Asetilkolinesteraz İnhibitörlerinin Değerlendirilmesi Değerlendirilmesi. Journal of Anatolian Environmental and Animal Sciences, 2026. https://doi.org/10.35229/jaes.1862726

AMA

1.Kızıl D. Doğu Karadeniz’in Önemli Bir Tarım Zararlısı Orosanga japonica (Melichar, 1898) (Homoptera: Ricaniidae)’ya Karşı Bitkisel Asetilkolinesteraz İnhibitörlerinin Değerlendirilmesi Değerlendirilmesi. JAES. 2026;(2026). doi:10.35229/jaes.1862726

Chicago

Kızıl, Demet. 2026. “Doğu Karadeniz’in Önemli Bir Tarım Zararlısı Orosanga Japonica (Melichar, 1898) (Homoptera: Ricaniidae)’ya Karşı Bitkisel Asetilkolinesteraz İnhibitörlerinin Değerlendirilmesi Değerlendirilmesi”. Journal of Anatolian Environmental and Animal Sciences, nos. 2026. https://doi.org/10.35229/jaes.1862726.

EndNote

Kızıl D (March 1, 2026) Doğu Karadeniz’in Önemli Bir Tarım Zararlısı Orosanga japonica (Melichar, 1898) (Homoptera: Ricaniidae)’ya Karşı Bitkisel Asetilkolinesteraz İnhibitörlerinin Değerlendirilmesi Değerlendirilmesi. Journal of Anatolian Environmental and Animal Sciences 2026

IEEE

[1]D. Kızıl, “Doğu Karadeniz’in Önemli Bir Tarım Zararlısı Orosanga japonica (Melichar, 1898) (Homoptera: Ricaniidae)’ya Karşı Bitkisel Asetilkolinesteraz İnhibitörlerinin Değerlendirilmesi Değerlendirilmesi”, JAES, no. 2026, Mar. 2026, doi: 10.35229/jaes.1862726.

ISNAD

Kızıl, Demet. “Doğu Karadeniz’in Önemli Bir Tarım Zararlısı Orosanga Japonica (Melichar, 1898) (Homoptera: Ricaniidae)’ya Karşı Bitkisel Asetilkolinesteraz İnhibitörlerinin Değerlendirilmesi Değerlendirilmesi”. Journal of Anatolian Environmental and Animal Sciences. 2026 (March 1, 2026). https://doi.org/10.35229/jaes.1862726.

JAMA

1.Kızıl D. Doğu Karadeniz’in Önemli Bir Tarım Zararlısı Orosanga japonica (Melichar, 1898) (Homoptera: Ricaniidae)’ya Karşı Bitkisel Asetilkolinesteraz İnhibitörlerinin Değerlendirilmesi Değerlendirilmesi. JAES. 2026. doi:10.35229/jaes.1862726.

MLA

Kızıl, Demet. “Doğu Karadeniz’in Önemli Bir Tarım Zararlısı Orosanga Japonica (Melichar, 1898) (Homoptera: Ricaniidae)’ya Karşı Bitkisel Asetilkolinesteraz İnhibitörlerinin Değerlendirilmesi Değerlendirilmesi”. Journal of Anatolian Environmental and Animal Sciences, no. 2026, Mar. 2026, doi:10.35229/jaes.1862726.

Vancouver

1.Demet Kızıl. Doğu Karadeniz’in Önemli Bir Tarım Zararlısı Orosanga japonica (Melichar, 1898) (Homoptera: Ricaniidae)’ya Karşı Bitkisel Asetilkolinesteraz İnhibitörlerinin Değerlendirilmesi Değerlendirilmesi. JAES. 2026 Mar. 1;(2026). doi:10.35229/jaes.1862726

EBSCOHost Scilit CABI

JAES/AAS-Journal of Anatolian Environmental and Animal Sciences/Anatolian Academic Sciences&Anadolu Çevre ve Hayvancılık Dergisi/Anadolu Akademik Bilimler-AÇEH/AAS

Evaluation of Plant-Based Acetylcholinesterase Inhibitors Against Orosanga japonica (Melichar, 1898) (Homoptera: Ricaniidae), an Important Agricultural Pest in the Eastern Black Sea Regionl Pest in the Eastern Black Sea Region

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Doğu Karadeniz’in Önemli Bir Tarım Zararlısı Orosanga japonica (Melichar, 1898) (Homoptera: Ricaniidae)’ya Karşı Bitkisel Asetilkolinesteraz İnhibitörlerinin Değerlendirilmesi Değerlendirilmesi

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