Interlaboratory Precision of Acute Toxicity Tests Using Reference Toxicant Formaldehyde

Yıl 2016, , 96 - 99, 24.12.2016

V. Zülal Sönmez , Nüket Sivri

https://doi.org/10.35229/jaes.277868

Cited By: 2

Öz

Acute
toxicity test, which takes place among rapid toxicity tests and is conducted
through bioluminescent bacteria, has been included in many countries' standards
thanks to its test duration, sample volume, cost-efficiency and result
sensitivity. Sensitivity of Microtox® Reagent, being one of the commercial kits
of bioluminescent bacteria, can be detected via reference toxicants (control).
Various reference toxicants (control) are recommended by the manufacturer
company to be used in Microtox® acute toxicity test. This study aims to use
formaldehyde as alternative reference toxicant (control) in Microtox® acute
toxicity test. Acute toxicity of formaldehyde, according to Vibrio fischeri,
was investigated on 5, 15 and 30 minutes of inhibition periods. EC₅₀
values were found as 2,30±0,60 mg/l; 2,14±0,63 mg/l and 2,17±0,70 mg/l by
inhibition periods, respectively. Standard deviation of
EC₅₀ values found for each sample group differed between 0,01-0,47.
According to mean acute toxicity results of all formaldehyde inhibition
periods, 0,10 unit deviation was found in 5 minutes of results. For 15 and 30
minutes of inhibition periods, mean value of unit deviation was found as 0,06
and 0,04 which showed closer values to each other and the mean value. The investigated toxicants show the performance of
pipetting sensitivity of reagent, analyzer and test operator.

Anahtar Kelimeler

Acute toxicity, Vibrio fischeri, Microtox®, reference toxicant, formaldehyde

Akut Toksisite Testi Hassasiyetinin Belirlenmesinde Referans Toksik Madde Olarak Formaldehit Kullanımı

Öz

Hızlı toksisite testlerinden; test süresi, numune hacmi,
uygun maliyeti ve sonuç hassasiyeti nedeniyle; biyolüminesans bakteri ile
yapılan akut toksisite testi, birçok ülke standartlarında yerini almıştır. Biyolüminesans
bakterinin ticari bir kiti olan Microtox® Reagent’ın hassasiyeti, referans toksik
madde (kontrol) sayesinde tespit edilebilmektedir. Microtox® akut toksisite
testinde kullanılmak üzere üretici firma tarafından farklı referans toksik
maddeler (kontrol)  önerilmektedir. Bu
çalışmada, Microtox® akut toksisite testinde kullanılmak üzere formaldehitin
alternatif referans toksik madde (kontrol) olarak kullanımı hedeflenmiştir.
Formaldehitin Vibrio fischeri’ye göre
olan akut toksisitesi, 5, 15 ve 30. dakika inhibisyon sürelerinde çalışılmıştır.
İnhibisyon sürelerine göre EC₅₀ değerleri sırasıyla 2,30±0,60 mg/l; 2,14±0,63
mg/l ve 2,17±0,70 mg/l bulunmuştur. Her bir örnekleme grubu için bulunan EC₅₀
değerlerinin, ortalamadan sapmaları ise 0,01-0.47 arasında değişim
göstermiştir. Formaldehitin tüm
inhibisyon süreleri için ortalama akut toksisite sonucuna bakıldığında da; 5.
dakikalık sonuçta 0,10 birim sapma görülmektedir. 15 ve 30 dakikalık inhibisyon
süreleri için ise; ortalama sırasıyla 0,06 ve 0,04 birim sapma ile ortalamaya
ve birbirlerine daha yakın değerler aldığı tespit edilmiştir. Çalışılan referans toksik maddeler; reagent, analizör ve
test operatörünün pipetleme hassasiyetinin performansını göstermektedir.

Anahtar Kelimeler

Akut toksisite, Vibrio fischeri, Microtox®, referans toksik madde, formaldehit

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