Usage of Lactobacillus rhamnosus as a Probiotic in Sea Bass (Dicentrarchus labrax)

Fatma Öztürk; Ömer Memduh Esendal

doi:10.35229/jaes.687057

EN

Usage of Lactobacillus rhamnosus as a Probiotic in Sea Bass (Dicentrarchus labrax)

Abstract

Bu çalışmada, levrek balıklarında (Dicentrarchus labrax) probiyotik olarak kullanılan Lactobacillus rhamnosus (ATCC 53103)’un deformasyon oranı, yaşama oranı, ağırlık artışı ve bağırsak mikroflorası üzerine etkisi araştırılmıştır. Bu amaçla, probiyotik etken son konsantrasyonu yetiştirme suyunda 106 kob/ml ve Artemia nauplii kültüründe ise 108 kob/ml olacak şekilde ilave edilmiştir. Bu şekilde probiyotik ilavesi 50. güne kadar devam etmiştir. Bu sürenin sonunda her iki grubun toz yemine 109 kob/g düzeyinde probiyotik ilavesi yapılmıştır. Deneme sonunda larvalardaki deformasyon oranları; yetiştirme suyuna probiyotik ilave edilen grupta %5, Artemia kültürüne ilave edilen grupta %2 ve kontrol grubunda %7 olarak tespit edilmiştir (P<0.01). Deneme gruplarının yaşama oranı kontrol grubuna kıyasla önemli düzeyde yüksek bulunmuştur (P<0.01). Probiyotik ilavesinin balıkların ağırlık artışları üzerinde önemli bir fark oluşturmadığı tespit edilmiştir (P>0.01). Ayrıca, Artemia kültürüne probiyotik ilavesinin Vibrio spp. sayısında önemli düzeyde azalmaya neden olduğu belirlenmiştir (P<0.01).

Keywords

Deformation,Lactobacillus rhamnosus,microflora,sea bass

References

Aubin, J., Gatesoupe, F.J., Labbé, L. & Lebrun, L. (2005). Trial of probiotics to prevent the vertebral column compression syndrome in rainbow trout (Oncorhynchus mykiss Walbaum). Aquaculture Research, 36(8), 758-767.
Byun, J.W., Park, S. & Benno, Y. (1997). Probiotic effect of Lactobacillus sp. DS–12 in flounder (Paralichthys olivaceus). Journal of General and Applied Microbiology, 43, 305-308.
Carnevali, O., de Vivo, L., Sulpizio, R., Gioacchini, G., Olivotto, I., Silvi, S. & Cresci, A. (2006). Growth improvement by probiotic in European sea bass juveniles (Dicentrarchus labrax, L.), with particular attention to IGF-1, myostatin and cortisol gene expression. Aquaculture, 258(1-4), 430-438.
Franke, A., Roth, O., De Schryver, P., Bayer, T., Garcia-Gonzalez, L., Künzel, S. & Clemmesen, C. (2017). Poly-β-hydroxybutyrate administration during early life: effects on performance, immunity and microbial community of European sea bass yolk-sac larvae. Scientific Reports, 7(1), 15022. Doi: 0.1038/s41598-017-14785-z
Gatesoupe, F.J. (1991). The effect of three strains of lactic bacteria on the production rate of rotifers, Brachionus plicatilis, and their dietary value for larval turbot, Scophthalmus maximus. Aquaculture, 96(3-4), 335-342.
Gil‐Martens, L. (2010). Inflammation as a potential risk factor for spinal deformities in farmed Atlantic salmon (Salmo salar L.). Journal of Applied Ichthyology, 26(2), 350-354.
Gobi, N., Vaseeharan, B., Chen, J.C., Rekha, R., Vijayakumar, S., Anjugam, M. & Iswarya, A. (2018). Dietary supplementation of probiotic Bacillus licheniformis Dahb1 improves growth performance, mucus and serum immune parameters, antioxidant enzyme activity as well as resistance against Aeromonas hydrophila in tilapia Oreochromis mossambicus. Fish & Shellfish Immunology, 74, 501-508. Doi: 10.1016/j.fsi.2017.12.066
Goda, A.M., Omar, E.A., Srour, T.M., Kotiet, A.M., El-Haroun, E. & Davies, S..J. (2018). Effect of diets supplemented with feed additives on growth, feed utilization, survival, body composition and intestinal bacterial load of early weaning European seabass, Dicentrarchus labrax post-larvae. Aquaculture International, 26(1), 169-183. Doi: 10.1007/s10499-017-0200-8d
Hameed, A.S. & Balasubramanian, G. (2000). Antibiotic resistance in bacteria isolated from Artemia nauplii and efficacy of formaldehyde to control bacterial load. Aquaculture, 183(3-4), 195-205.
Hamza, A., Fdhila, K., Zouiten, D. & Masmoudi, A.S. (2016). Virgibacillus proomii and Bacillus mojavensis as probiotics in sea bass (Dicentrarchus labrax) larvae: effects on growth performance and digestive enzyme activities. Fish Physiology and Biochemistry, 42(2), 495-507. Doi: 10.1007/s10695-015-0154-6

Harrigan, W.F (1998). Laboratory Methods in Food Microbiology. 3rd ed. San Diego, CA, USA: Academic Press.
Harzevili, A.R., Duffel, H.V., Dhert, P., Swings, J., and Sargeloos, P. (1998). Use of potential probiotic Lactococcus lactis AR21 strain for the enhancement of growth in the rotifer Brachionus plicatilis. Aquaculture Research, 29, 411-417. Doi: 0.1046/j.1365-2109.1998.00217.x
Jöborn, A., Olsson, J.C., Westerdahl, A., Conway, P.L. & Kjelleberg, S. (1997). Colonization in the fish intestinal tract and production of inhibitory substances in intestinal mucus and faecal extracts by Carnobacterium sp. strain K1. Journal of Fish Disease, 20, 383-392. Doi: 10.1046/j.1365-2761.1997.00316.x
Lamari, F., Castex, M., Larcher, T., Ledevin, M., Mazurais, D., Bakhrouf, A. & Gatesoupe, F.J. (2013). Comparison of the effects of the dietary addition of two lactic acid bacteria on the development and conformation of sea bass larvae, Dicentrarchus labrax, and the influence on associated microbiota. Aquaculture, 376, 137-145. Doi: 10.1016/j.aquaculture.2012.11.016
Lin, H.L., Shiu, Y.L., Chiu, C.S., Huang, S.L. & Liu, C.H. (2017). Screening probiotic candidates for a mixture of probiotics to enhance the growth performance, immunity, and disease resistance of Asian seabass, Lates calcarifer (Bloch), against Aeromonas hydrophila. Fish & Shellfish Immunology, 60, 474-482. Doi: 10.1016/j.fsi.2016.11.026.
Ljubobratovic, U., Kosanovic, D., Vukotic, G., Molnar, Z., Stanisavljevi, N.S., Ristovic, T. & Jeney, G. (2017). Supplementation of lactobacilli improves growth, regulates microbiota composition and suppresses skeletal anomalies in juvenile pike-perch (Sander lucioperca) reared in recirculating aquaculture system (RAS): a pilot study. Research in Veterinary Science, 115, 451-462. Doi: 10.1016/j.rvsc.2017.07.018
Muroga, K., Higashi, M. & Keitoku, H. (1987). The isolation of intestinal microflora of farmed red sea bream (Pagrus major) and black sea bream (Acanthopagrus schlegeli) at larval and juvenile stages. Aquaculture, 65, 79-88.
Nguyen, T.L., Park, C.I. & Kim, D.H. (2017). Improved growth rate and disease resistance in olive flounder, Paralichthys olivaceus, by probiotic Lactococcus lactis WFLU12 isolated from wild marine fish. Aquaculture, 471, 113-120. Doi: 10.1016/j.aquaculture.2017.01.008
Nikoskelainen, S., Ouwehand, A.C., Salminen, S. & Bylund, G. (2001). Protection of rainbow trout (Oncorhynchus mykiss) from furunculosis by Lactobacillus rhamnosus. Aquaculture, 198, 229-236.
Olsen, A.I., Olsen, Y., Attramadal, M.Y., Chiristie, K., Birkbeck, T.H., Skjermo, J. & Vadstein, O. (2000). Effect of short term feeding of microalgae on the bacterial flora associated with juvenile Artemia franciscana. Aquaculture, 190, 11-25.
Piccolo, G., Bovera, F., Lombardi, P., Mastellone, V., Nizza, S., Meo, C.D. & Marono, S. (2015). Effect of Lactobacillus plantarum on growth performance and hematological traits of European sea bass (Dicentrarchus labrax). Aquaculture International, 23(4), 1025-1032. Doi: 10.1007/s10499-014-9861-8
Reitan, K.I., Rainuzzo, J.R. & Olsen, Y. (1993). Nutritional effects of algal addition in first feeding tanks of turbot (S. maximus) larvae. Aquaculture, 118, 257-275.
Rollo, A., Sulpizio, R., Nardi, M., Silvi, S., Orpianesi, C., Caggiano, M. & Carnevali, O. (2006). Live microbial feed supplement in aquaculture for improvement of stress tolerance. Fish Physiology and Biochemistry, 32(2), 167-177. Doi: 10.1007/s10695-006-0009-2
Silvi, S., Nardi, M., Sulpizio, R., Orpianesi, C., Caggiano, M., Carnevali, O. & Cresci, A. (2008). Effect of the addition of Lactobacillus delbrueckii subsp. delbrueckii on the gut microbiota composition and contribution to the well-being of European sea bass. Microbiol Ecology in Health and Disease, 20(1), 53-59. Doi: 10.1080/08910600701876020
Sutthi, N., Thaimuangphol, W., Rodmongkoldee, M., Leelapatra, W. & Panase, P. (2018). Growth performances, survival rate, and biochemical parameters of Nile tilapia (Oreochromis niloticus) reared in water treated with probiotic. Comparative Clinical Pathology, 27(3), 597-603. Doi: 10.1007/s00580-017-2633-x
Tovar-Ramirez, D., Infante, Z.J., Cahu, C., Gatesoupe, F.J., Vazquez-Juarez, R. (2004). Influence of dietary live yeast on European sea bass larval development. Aquaculture, 234, 415-427. Doi:10.1016/j.aquaculture.2004.01.028
Van Doan, H., Hoseinifar, S. H., Khanongnuch, C., Kanpiengjai, A., Unban, K. & Srichaiyo, S. (2018). Host-associated probiotics boosted mucosal and serum immunity, disease resistance and growth performance of Nile tilapia (Oreochromis niloticus). Aquaculture, 491, 94-100. Doi: 10.1016/j.aquaculture.2018.03.019
Varsamos, S., Connes, R., Diaz, J. P., Barnabé, G. & Charmantier, G. (2001). Ontogeny of osmoregulation in the European sea bass Dicentrarchus labrax L. Marine Biology, 138(5), 909-915.
Vendrell, D., Balcázar, J.L., de Blas, I., Ruiz-Zarzuela, I., Gironés, O. & Múzquiz, J.L. ( 2008). Protection of rainbow trout from lactococcosis by probiotic bacteria. Comparative Immunology, Microbiology & Infectious Diseases, 31, 337-345. Doi:10.1016/j.cimid.2007.04.002
Villamil, L, Figueras, A., Planas, M. & Novoa, B. (2010). Pediococcus acidilactici in the culture of turbot larvae: administration pathways. Aquaculture, 307(1-2), 83-88. Doi:10.1016/j.aquaculture.2010.07.004

Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Fatma Öztürk ^*
0000-0003-4763-3801
Türkiye

Ömer Memduh Esendal
0000-0002-2700-2634
Kuzey Kıbrıs Türk Cumhuriyeti

Publication Date

March 31, 2020

Submission Date

February 10, 2020

Acceptance Date

March 13, 2020

Published in Issue

Year 2020 Volume: 5 Number: 1

DOI

https://doi.org/10.35229/jaes.687057

IZ

https://izlik.org/JA22NL63CB

APA

Öztürk, F., & Esendal, Ö. M. (2020). Usage of Lactobacillus rhamnosus as a Probiotic in Sea Bass (Dicentrarchus labrax). Journal of Anatolian Environmental and Animal Sciences, 5(1), 93-99. https://doi.org/10.35229/jaes.687057

EBSCOHost Scilit CABI

JAES/AAS-Journal of Anatolian Environmental and Animal Sciences/Anatolian Academic Sciences&Anadolu Çevre ve Hayvancılık Dergisi/Anadolu Akademik Bilimler-AÇEH/AAS

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Usage of Lactobacillus rhamnosus as a Probiotic in Sea Bass (Dicentrarchus labrax)

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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