Oreochromis niloticus'un Karaciğerinde Fungisit Propiconazole'un Oksidatif Stres Parametreleri ve Antioksidan Sistem Enzimleri Üzerine Etkileri

Yıl 2019, Cilt: 4 Sayı: 1, 43 - 47, 30.04.2019

Ozge Temiz

https://doi.org/10.35229/jaes.532079

Cited By: 2

Öz

Bu
Araştırmada, fungisit propiconazole’un 0.5 ppb ve 2.5 ppb derişim etkilerinde 7
gün ve 28 gün sürelerde Oreochromis
niloticus’un karaciğer dokusunda, süperoksit dismutaz (SOD), katalaz (CAT),
glutatyon peroksidaz (GPx) ve glutatyon S transferaz (GST) antioksidan sistem
enzim aktiviteleri ve oksidatif stres parametreleri glutatyon (GSH) ve
tiyobarbitürik asit reaktif maddeleri (TBARS) miktarlarındaki değişimler
spektrofotometrik yöntemlerle belirlenmiştir.

Çalışmamızda
karaciğer dokusunda GSH miktarı ve GSH-bağımlı GPx ve GST aktivitesi 7. günde
artarken; 28. günde GSH miktarında azalma ve GST aktivitelerinde artış
belirlenmiştir. SOD ve CAT aktiviteleri 7. günde artarken 28. günde azalmıştır.
TBARS düzeylerinde propiconazole etkisinde süreye ve derişime bağlı olarak
artış göstermektedir.

Çalışma
sonuçlarına göre propiconazole etkisinde O.
niloticus’da antioksidan sistem enzim aktivitelerinde ve oksidatif stres
parametrelerindeki değişimler propiconazole’un hepatotoksik etkiye neden olduğu
belirlenmiştir.

Anahtar Kelimeler

Fungisit, Propiconazole, Oreochromis niloticus, Karaciğer, Antioksidan sistem, Glutatyon

Effects of Fungicide Propiconazole on Oxidative Stress Parameters and Antioxidant System enzymes in liver of Oreochromis niloticus

Öz

In
this research, 0.5 ppb and 2.5 ppb effect of fungicide propiconazole in 7 days
and 28 days periods in liver tissue of Oreochromis
niloticus, superoxide dismutase (SOD), catalase (CAT), glutathione
peroxidase (GPx) and glutathione S
transferase (GST) antioxidant system enzyme activities and oxidative stress
parameters glutathione (GSH) and thiobarbituric acid reagents (TBARS) were
determined by spectrophotometric methods.

In
our study, GSH levels and GSH-dependent GPx and GST activity increased in 7
days. In 28 days, decrease in GSH and increase in GST activities were
determined. SOD and CAT activities increased in 7 days and decreased in 28
days. In TBARS levels, the time and concentrations -related increase in the
effect of propiconazole

According
to the results of the study; The effect of propiconazole on antioxidant enzyme
activity and oxidative stress parameters in O.
niloticus was determined to cause hepatotoxic effect.

Anahtar Kelimeler

Fungicide, Propiconazole, glutathion, antioxidant system, liver

Kaynakça

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