Cyprinus carpio’da Bakırın Solungaç Dokusunda Birikimi ve Na/K İyon Düzeylerine Etkisi

Yıl 2020, Cilt: 5 Sayı: 3, 313 - 317, 30.09.2020

Hikmet Yeter Çoğun , Ferit Kargın

https://doi.org/10.35229/jaes.749347

Cited By: 3

Öz

Bu çalışmada farklı bakır ortam derişimlerinin etkisine farklı sürelerle bırakılan Cyprinus carpio’nun solungaç dokusunda bakır birikimi ve bakırın Na+ ve K+- iyon düzeylerine etkileri incelenmiştir. Balıklar 0.1, 0.5 ve 1.0 mg/L Cu derişimlerine 10, 20 ve 30 günlük sürelerle bırakılarak solungaç dokularındaki bakır birikimi ile sodyum ve potasyum iyon düzeyleri Atomik Absorbsiyon Spektrofotometrik yöntemle saptanmıştır. Bakırın 1.0 mg/L derişiminin etkisinde 30. gün sonunda balıkların tümü ölmüştür. C. carpio’nun solungaç dokusunda bakır birikimi derişim artışına ve sürenin uzamasına bağlı olarak arttığı belirlenmiştir. C. carpio’da denenen tüm koşullarda bakır, solungaç Na+ ve K+ düzeyini azaltmıştır.

Anahtar Kelimeler

Cyprinus carpio, Bakır, solungaç dokusu

Destekleyen Kurum

Çukurova Üniversitesi Bilimsel Araştırma Projeleri birimi (BAP)

Proje Numarası

FEF2003D13

Teşekkür

Bu çalışma Çukurova Üniversitesi Bilimsel Araştırma Projeleri birimi (BAP) tarafından desteklenmiştir (Proje No: FEF2003D13).

Accumulation of Copper in Gill Tissue and Its Effect on Na / K Ion Levels in Cyprinus carpio

Öz

In this study, copper accumulation in the gill tissue of Cyprinus carpio, which was exposed to the effect of different copper media concentrations for different periods, and the effects of copper on Na + and K + - ion levels were investigated. The fish were dropped to 0.1, 0.5 and 1.0 mg / L Cu concentrations for 10, 20 and 30 days, and copper accumulation and sodium and potassium ion levels in the gill tissues were determined by Atomic Absorption Spectrophotometric method. All fish died at the end of the 30th day under the effect of copper 1.0 mg / L concentration. It was determined that copper accumulation in the gill tissue of C. carpio increased due to the increase in concentration and the prolongation of the time. In all conditions tested in C. carpio, copper decreased gill Na + and K + levels.

Anahtar Kelimeler

C. carpio, Copper, accumulation

Proje Numarası

FEF2003D13

Kaynakça

• Astorga-Espana, M. S., Pena-Mendez, E. M. & Montelongo F. J. (1999). Application of principal companent analysis to the study of major cations and trace metals in fish from Tenefire (Canary Islands), Chem. And İntell. Lab. 49, 173-178.
• Bjerregaard, P. and Vislie, T. (1985). Effect of mercury on ion and osmoregulation in the shore Crab Carcinus maenas (L.), Comp. Biochem. Physiol. 82C, 1, 227-230.
• Buckley, J. T., Roch, M., Mccarter, J. A., Rendell, C. A. & Matherson, A. T. (1982). Chronic exposure of Coho Salmon to sublethal concentrations of copper-I. effects of growth, on accumulation and distribution of copper and on copper tolerance. Comp. Biochem. Physiol., 72 C (1): 15-19. Campana, O., Sarasquete, C. & Blasco, J. (2003). Effect of lead on ALA-D activity, metallothionein levels, and lipid peroxidation in blood, kidney, and liver of the Toadfish Halobatrachus didactylus. Ecotoxicology and Environmental Safety. 55, 116-125. Cearley, J. E. & Coleman, R. L. (1974). Cadmium toxicity and bioconcentration in laregmouth bass and bluegill. Bulletin of Environmental Contamination and Toxicology, 11, 146-151. Croke, S. J. & Mcdonald, D. G. (2002). The further development of ionoregulatory measures as biomarkers of sensitivity and effect in fish species, Environ. Toxicol. Chemist, 21, 8, 1683-1691.
• Çoğun, H & Kargın, F . (2019). Oreochromis niloticus’un solungaç dokusu iyon düzeyleri üzerine kurşunun etkisi. Journal of Anatolian Environmental and Animal Sciences, 4 (1) , 22-26 . DOI: 10.35229/jaes.527071
• Depledge, M. H., Aagaard, A. & Gyorkos, P. (1995). Assessment of Trace Metal Toxicity Using Molecular, Physiological and Behavioural Biomarkers. Marine Pollut. Bull., 31: 19-27.
• Erdem, C. & Kargin, F. (1992). a comparative study on the accumulation of copper in liver, spleen, stomach, ıntestine, gill and muscle tissues of Cyprinus carpio and Tilapia nilotica. Biyokimya Dergisi, XVII, (1), 13-27.
• Flik, G. & Verbost, P. M. (1993). Calcium transport in fish gills and ıntestine. J. Exp. Biol. 184, 17-29.
• Hawkins, A. D. (1981). Aquarium systems, Academic Pres. London. 452 pp.
• Heath, A. G. (1987). Water Pollution and Fish Physiology. CRC Press. 24 pp. Florida USA.
• Hilmy, A. M., Shabana, M. B. & Daabees, A. Y. (1985). Bioaccumulation of cadmium: toxicity in Mugil cephalus. Comp. Biochem. Physiol. 81C(1), 139-143.
• Lacrox, G.L., Gordon, D.J. & Johnstan, D.J. (1985). Effects of low environmental pH on the survival, growth, and ıonic composition of postemergent Atlantic Salmon (Salmo salar). Can. J. Fish. Aquat. Sci, 42, 768-775.
• Lam, K. L., Ko, P. W. & Judy, K. Y. (1998). Metal toxicity and metallothionein gene expression studies in Carp and Tilapia. Marine Environ. Res. 46, 563-566.
• Lauren, D.J. & Mc Donald, D.G. (1987). Acclimation to copper by Rainbow Trout, Salmo gairdneri: Physiology. Can. J. Fish. Aquat. Sci. 44: 99-104.
• McDonald, A., Silk, L., Schwartz, M. & Playle, R. C. (2002). A lead-gill binding model to predict acute lead toxicity to Rainbow Trout (Oncorhynchus mykiss). Comp. Biochem. Physiol., C. 133, 227-242.
• McDonald, D. G., Tang, Y.& Boutilier, R. G. (1989). Acid and ıon transfer across the gills of fish: mechanisms and regulation. Can. J. Zool., 67, 3046-3054.
• McGeer, J. C., Szebedınszky C., McDonald D. G. & Wood C. M. (2000). Effect of chronic sublethal exposure to waterborne Cu, Cd or Zn in Rainbow trout 2: tissue spesific metal accumulation. Aquatic Toxicology, 50, 245-256.
• Munoz, M. J., Carballo, M. & Tarazona, J. V. (1991). The Effect of sublethal levels of copper and cyanide on some biochemical parameters of Rainbow Trout along subacute explotion. Comp. Biochem. Physiol, 100C (3), 577-582.
• Muramoto, S. (1983). Elimination of Copper from Cu-contaminated fish by long-term exposure to EDTA and fresh-water. J. Environ. Sci. Health A, 18 (3), 455-461.
• Murphy, C. B. Jr, and Spiegel, S. J. (1983). Bioaccumulation and toxicity of heavy metals and related trace elements, Water Pollution, 55, 6, 816-821.
• Pelgrom, S. M. G. J., Lock, R. A. C., Balm, P. H. M. & Wendelaar Bonga, S. E. (1995). Effects of Combined Waterborne Cd and Cu Exposures on Ionic Composition and Plasma Cortisol in Tilapia, Oreochromis mossambicus. Comp. Biochem. And Physiol. P. C: Toxicol. And Endocrin. 111, 2, 227-235.
• Roger, J. T., Rıchards, J. G. & Wood, C. M. (2003). Ionoregulatory disruption as the toxic mechanism for lead in the Rainbow Trout (Oncorhnchus mykiss). Aquatic Toxicology. 64(2), 215-234.
• Satyavathi, C. & Rao, Y. P. (2000). Inhibition of Na+, K+-ATPase in Penaeus indicus postlarvae by lead. Comp. Biochem. Physiol., C. 127, 11-22.
• Suresh, A., Sivaramakrishna, B. & Radhakrishnaiah, K. (1995). Cadmium induced changes in ıon levels and ATPase activities in the muscle of the fry and fingerlings of the freshwater fish, Cyprinus carpio. Chemosphere, 30, 2, 365-375.
• Thaker, J., Chhaya, J., Nuzhat, S., Mittal, R., Mansuri, A. P. & Kundu, R. (1996). Effects of chromium (VI) on some ion-dependent ATPases in gills, kidney and ıntestine of a Coastal Teleost Periophtalmus dipes. Toxicology. 112, 237-244.
• Viarengo, A. (1985). Biochemical Effects of Trace Metals. Marine Pollution Bull, 16, 4, 153-158.
• Viarengo, A. (1989). Heavy metals in marine ınvertebrates. mechanism of regulation and toxicity at the cellular level. Aquatic Sciences, 1 (2), 295–317.
• Wang, T., Knudsen, P. K., Brauner, C. J, Busk, M., Vijayan, M. M. & Jensen, F. B. (1998). Copper exposure impairs intra-and extracelluler acid-base regulation during hypercapnia in the fresh water Rainbow Trout (Oncorhynchus mykiss). J. Comp. Physiol., B, 168, 591-599.