Sıcaklık Şoku Uygulaması ile Triploid Karadeniz Alabalığı (Salmo trutta labrax) Üretimi ve Kuluçka Performansının Belirlenmesi[*]

Yıl 2021, Cilt: 6 Sayı: 4, 635 - 641, 31.12.2021

Fatma Delihasan Sonay , Nadir Başçınar , Süleyman Akhan

https://doi.org/10.35229/jaes.957507

Öz

Bu çalışmada, triploid Karadeniz alabalığı (Salmo trutta labrax) üretmek için termal şok protokolünün optimizasyonu belirlenmiştir. Triploidizasyon işlemi için döllenmeden sonra 10, 15 ve 20. dakikalarda dört farklı sıcaklıkta (26, 28, 30 ve 32 ºC) şok uygulanmıştır. Ploidi oranı NOR (Nükleolar Organizatör Bölgeleri) boyama ve eritrosit büyüklüğü yöntemleri ile belirlenmiştir. En yüksek triploid oranları, döllendikten 15 dakika sonra 10 dakika sıcaklık şoku uygulamasıyla 32 ºC (%86,15) ve 28 ºC (%81,26)’lerde tespit edilmiştir. Fakat, çıkış oranı ve kuluçka randımanı 28 ºC sıcaklık şoku grubunda daha başarılı bulunmuştur. Çalışmadan elde edilen bulgular, Karadeniz alabalığı triploid uygulamalarında kullanılabilir.

Anahtar Kelimeler

Salmo trutta labrax, triploid oranı, NOR, eritrosit büyüklüğü

Destekleyen Kurum

Karadeniz Teknik Üniversitesi

Proje Numarası

2009.117.001.5 (922)

Triploid Black Sea Trout (Salmo trutta labrax) Production by Using Heat Shocks and Determination of Incubation Performance

Öz

In this study, optimization of thermal shock protocol for triploid Black Sea trout (Salmo trutta labrax) production were determined. In total, four different temperature regimes (26, 28, 30, and 32 ºC) were performed following fertilization at 10th, 15 th, and 20 th minutes for triploidization process. Ploidy rate was determined by NOR (Nucleolus Organizer Regions) and measures of erythrocyte size. The highest rate of triploidy was detected with a shock treatment at 32 °C (86,15%) and 28°C (81,26%) for 10 min shock duration initiated 15 min post-fertilization. However, hatching rate and hatching efficiency were more successful at the 28 ºC heat shock group. The results of the present study is applicable to the production of triploid Black sea trout production.

Anahtar Kelimeler

Salmo trutta labrax, triploid rate, NOR, erythrocyte size

Proje Numarası

2009.117.001.5 (922)

Kaynakça

• Akhan, S., Serezli, R. & Delihasan Sonay, F. (2011a). Hematology of diploid and triploid rainbow trout (Oncorhynchus mykiss), Black Sea trout (Salmo labrax Pallas, 1814) and their F1 hybrids. The Israeli Journal of Aquaculture, Bamidgeh, 63, 4.
• Akhan, S., Delihasan Sonay, F., Okumus, I., Köse, Ö. & Yandi, I. (2011b). Inter-specific hybridization between Black Sea trout (Salmo labrax Pallas, 1814) and rainbow trout (Oncorhynchus mykiss Walbaum, 1792). Aquaculture Research, 42, 1632–1638. doi: 10.1111/j.1365-2109.2010.02755.x
• Aksungur, M., Yılmaz, C., Tabak, İ., Aksungur, N. & Mısır, D.S. (2005). Karadeniz alabalığı (Salmo trutta labrax, 1811)’nın kültür şartlarına adaptasyonu. Fırat Üniversitesi, Fen ve Mühendislik Bilimleri Dergisi, 17, 349-359.
• Arai, K. & Wilkins, N. (1987). Triploidization of Brown trout (Salmo trutta) by heat shocks. Aquaculture, 64, 97-103.
• Bai, Z., Liu, F., Li, J. & Yue, G.H. (2011). Identification of triploid individuals and clonal lines in Carassius auratus complex using microsatellites. International Journal of Biological Sciences, 7(3), 279-285.
• Baki, B. (2006). Gökkuşağı alabalıklarından (Oncorhynchus mykiss, W., 1792) elde edilen yumurtaların iki farklı su kaynağında açılma süreleri, larva çıkışı ve büyümelerinin karşılaştırılması. Ondokuz Mayıs Üniversitesi Fen Bilimleri Enstitüsü, Samsun, Türkiye, 88 s.
• Blanc, J.M., Vallée, F. & Dorson, M. (2000). Survival, growth and dressing traits of triploid hybrids between rainbow trout and three charr species, Aquaculture Research, 31, 349-358.
• Cal, R., M., Vidal, S., Camacho, T., Piferrer, F. & Guitian, F., J. (2005). Effect of Triploidy on Turbot Haematology, Comparative Biochemistry and Physiology, Part A 141, 35-41.
• Canyurt, M.A. & Akhan, S. (2009). Development and situation of trout culture in Turkey. pp. 90-94. In: 15th Annu. Int. Sci. Conf.: Research for Rural Development 2009. 20-22 May 2009, Jelgava, Latvia.
• Crozier, W.W. & Moffett, I.J.J. (1989). Experimental Production of Triploid Brown Trout, Salmo trutta L., Using Heat Shock. Aquaculture and Fisheries Management, 20, 343-353.
• Çakmak, E., Çankırılıgil, E.C., Düzgüneş, Z.D., Özel, O.T., Eroğlu, O. & Firidin, Ş. (2019). Triploid Black Sea Trout (Salmo labrax Pallas, 1814) Induced by Heat Shock and Evaluation of Triploidy with Different Techniques. GenAqua, 3(1), 01-07.
• Dillon, J.C. (1988). Production of Triploid Rainbow Trout for Evaluation in South Dakota Waters. The Degree Master of Science, South Dakota State University, Wildlife and Fisheries Sciences. South Dakota. 69 p.
• Dorafshan, S., Kalbassi, M.R., Pourkazemi, M., Amiri, B.M. & Karimi, S.S. (2008). Effects of triploidy on the Caspian salmon Salmo trutta caspius haematology. Fish Physiology and Biochemistry, 34, 195-200.
• Dorota, F., B., Jankun, M. & Wosnicki, P. (2006). Chromosome number and erythrocyte nuclei length in triploid Siberian sturgeon Acipenser baeri Brandt. Caryologia, 59 (4), 319-321.
• Dubé, P., Blanc, J.M., Chouinard, M. & Noüe, J. (1991). Triploidy induced by heat shock in brook trout (Salvelinus fontinalis). Aquaculture, 92, 305-311. Felip, A., Zanuy, S., Carrillo, M., Martinez, G., Ramos, J. & Piferrer, F. (1997). Optimal Conditions Fort The Induction of Triploidy in the Sea bass (Dicentrarchus labrax L.), Aquaculture, 152, 287-298.
• Felip, A., Zanuy, S., Carrillo, M. & Piferrer, F. (2001). Induction of triploidy and gynogenesis in teleost fish with emphasis on marine species. Genetica, 111, 175-195.
• Gao, Z., Wang, W., Abbas, K., Zhou, X., Yang, Y., Diana, J.S., Wang, H., Wang, H., Li, H. & Sun, Y. (2007). Haematological characterization of loach Misgurnus anguillicaudatus: Comparison among diploid, triploid and tetraploid specimens. Comparative Biochemistry and Pysiology, Part A147, 1001-1008.
• Gray, A., K., Evans, M., A., & Thorgaard, G., H. (1993). Viability and Development of Diploid and Triploid Salmonid Hybrids. Aquaculture, 112, 125-142.
• Gündoğdu, H. (2016). Endemik Beyşehir Kababurun Balığı, Chondrostoma beysehirense (Bogutskaya, 1997) Üzerine Sitogenetik Araştırmalar. Selçuk Üniversitesi Fen Bilimleri Enstitüsü. Konya, Türkiye, 43s.
• Happe, A., Quillet, E. & Chevassus, B. (1988). Early life history of triploid rainbow trout (Salmo gairdneri Richardson). Aquaculture, 71, 107-118.
• Hisar, S.A., Yanık, T. & Hisar, O. (2003). Hatchery and growth performance of two trout pure breeds, Salvelinus alpinus and Salmo trutta fario, and their hybrid. The Israeli Journal of Aquaculture-Bamidgeh, 55 (3), 154-159.
• Howell, W.M. & Black, D.A. (1980). Controlled Silver-Staining of Nucleolar Organizer Regions With A Protective Colloidal Developer: A 1-step Method. Experienta, 36, 1014-1015.
• Jankun, M., Woznicki, P., Dajnowicz, G., Demska-Zakes, K., Luczynski, M.J. & Luczynski, M. (1998). Heterochromatin and NOR location in Northern Pike (Esox lucius), Aquatic Science, 60, 17-21.
• Jankun, M., Kuzminski, H. & Furgala-Selezniow, G. (2007). Cytologic ploidy determination in fish–an example of two salmonid species. Environmental Biotechnology, 3 (2), 52–56.
• Johnson, R.M., Shrimpton, J.M., Heath, J.W. & Heath, D.D. (2004). Family, induction methodology and interaction effects on the performance of diploid and triploid chinook salmon (Oncorhychus tshawytscha). Aquaculture, 234, 123-142.
• Kalbassi, M.R., Dorafshan, S., Pourkazemi, M. & Amiri, B.M. (2009). Triploidy induction in the Caspian Salmon, Salmo trutta caspius, by heat shock. Journal of Applied Ichthyology, 25, 104-107.
• Kankaya, E. (1998). Gökkuşağı Alabalığında (Oncorhynchus mykiss W., 1792) Isı Şoku Uygulamasıyla Triploidi Oluşturulması Üzerine Bir Araştırma. Yüksek Lisans Tezi, Yüzüncü Yıl Üniversitesi, Fen Bilimleri Enstitüsü, 54 s.
• Katsutoshi, A. & Wilkins, N.P. (1987). Triplodization of Brown Trout ( Salmo trutta) by Heat Shock. Aquaculture, 64, 97-103.
• Kenanoğlu, O.N., Yilmaz, S., Ergun, S. & Aki, C. (2013). A preliminary study on the determination of triploidy by chromosome analysis at the different stages of development in rainbow trout, Oncorhynchus mykiss. Marine Science and Technology Bulletin, 2 (2), 17-21.
• Kızak, V., Güner, Y., Türel, M. & Kayım, M. (2013). Comparison of Growth Performance, Gonadal Structure and Erythrocyte Size in Triploid and Diploid Brown Trout (Salmo trutta fario L, 1758). Turkish Journal of Fisheries and Aquatic Sciences, 13, 571-580. Doi: 10.4194/1303-2712-v13_4_02
• Kim, H.S., Chung, K.H. & Son, J.H. (2017). Comparison of different ploidy detection methods in Oncorhynchus mykiss, the rainbow trout. Fisheries and Aquatic Sciences, 20, 29. Doi:10.1186/s41240-017-0074-8
• Liu, F.G. & Quillet, E. (1989). Preliminary results on triploidy induced bu heat shocks in the brown trout (Salmo trutta). Journal of the Fisheries Society of Taiwan (JFST). 16 (2), 91-95.
• Lozano, R., Rejón, C.R. & Rejón, M.R. (1992). A comparative analysis of NORs in diploid and triploid salmonids: implications with respect to the diploidization process occurring in this fish group. Heredity. 69, 450-457.
• Ojolick, E.J., Cusack, R., Benfey, T.J. & Kerr, S.R. (1995). Survival and growth of all-female diploid and triploid rainbow trout (Oncorhynchus mykiss) reared at chronic high temperature. Aquaculture, 131, 177-187.
• Okumura, S.I., Furukawa, S., Kawai, T., Takahashi, S. & Yamamori, K. (2001). Comparison of nucleoli number in diploid and triploid larva of Pacific abalone Haliotis discus hannai. Fisheries Science, 67, 176–178.
• Peruzzi, S., Varsamos, S., Chatain, B., Fauvel, C., Menu, B., Falguière, C., Sévère, A. & Flik, G. (2005). Haematological and Physiological Characteristics of Diploid and Triploid Sea Bass, Dicentrarchus labrax L. Aquaculture, 244 (1-4), 359-367.
• Piferreer, F., Cal, R., Àlvarez-Blázquez, B., Sánchez, L. & Martinez, P. (2000). Introduction of triploidy in the turbot (Scophthalmus maximus) I. Ploidy determination and the effects of cold shocks. Aquaculture, 188, 79-90.
• Piferreer, F., Cal, R., Gómez, C., Bouza, C. & Martinez, P., 2003. Introduction of Triploidy in The Turbot (Scophthalmus maximus) II.Effects of Cold Shock Timing and Induction of Triploidy in a Large Volume of Eggs, Aquaculture, 220, 821-831.
• Quillet, E., Foisil, L., Chevassus, B., Chourrout, D. & Liu, F.G. (1991). Production of All-triploid and All-female Brown Trout for Aquaculture. Aquatic Living Resources, 4, 27-32.
• Saygun, S. (2005). Karadeniz’de yaşayan çeşitli yassı balıkların (Pisces, Pleuronectiformes) kromozom yapılarının karşılaştırılması. On Dokuz Mayıs Üniversitesi Fen Bilimleri Enstitüsü. Samsun, Türkiye, 147s.
• Serezli, R., Okumuş, İ. & Akhan, S. (2005). Akuakültürde Anestezinin Kullanımı. Türk Sucul Yaşam Dergisi, 4, 475-480.
• Sola, L., Bressanello, S., Rossi, A., R., Iaselli,V., Crosetti, D. & Cataudella, S. (1993). A Karyotype Analysis of The Genus Dicentrarchus by Different Staining Techniques, Journal of Fish Biology, 43, 329-337.
• Solar, I.I., Donaldson, E.M. & Hunter, G.A. (1984). Induction of triploidy in rainbow trout (Salmo gairdneri RICHARDSON) by heat shock, and investigation of early growth. Aquaculture, 42, 57-67. Sonay, F. (2013). Triploid Karadeniz Alabalığı (Salmo trutta labrax Pallas, 1811) Üretimi Ve Büyüme Potansiyeli Ve Et Kalitesinin Belirlenmesi. Karadeniz Teknik Üniversitesi Fen Bilimleri Enstitüsü. Trabzon, Türkiye, 137s.
• URL 1. http://ressources.ciheam.org/om/pdf/b63/00800917pdf
• Valenti, R.J. (1975). Induced polyploidy in Tilapia aurea (Steindachner) by means of temperature shock treatment. Journal of Fish Biology, 7, 519-528.
• Wolters, W., R., Chrisman, C., L. & Libey, G.S. (1982). Erythrocyte Nuclear Measurement of Diploid Triploid Channel Catfish, Ictalurus punctatus (Rafinesque). Journal of Fish Biology, 20, 253-258.
• Woznicki, P. & Kuzminski, H. (2002). Chromosome Number and Erythrocyte Nuclei Length in Triploid Brook Trout (Salvelinus fontinalis). Caryologia, 55 (4), 295-298.
• Yılmaz, S., Çelik, E.Ş., Kenanoğlu, O.N. & Ergün, S. (2017). Asidik stresin triploid ve diploid gökkuşağı alabalığı (Oncorhynchus mykiss)'nın hematolojik, immunolojik ve biyokimyasal kan parametreleri üzerine etkileri. Alınteri Journal of Agricultural Sciences, 32(2),17-24. Doi: 10.28955/alinterizbd.320262