Investigation of Apoptosis-mediated Cytotoxic Effects of Royal Jelly on HL-60 Cells

Yıl 2024, Cilt: 9 Sayı: 4, 514 - 520

Hasan Ulusal , Mehmet Akif Bozdayı , Hatice Kübra Yigit Dumrul , Melek Sena Tarakçıoğlu , Abdullah Taşkın

https://doi.org/10.35229/jaes.1550189

Öz

In recent years, the use of nontoxic natural products that can be effective on cancer cells as new agents has attracted the attention of scientists in order to reduce the negative side effects of existing cancer drugs and their toxicity to normal cells. Some in vivo and in vitro studies have shown that royal jelly (RJ) inhibits cell proliferation and induces apoptosis. In this research, we aimed to investigate the effects of RJ on proliferative and apoptotic processes in the human acute promyelocytic leukemia cell line (HL-60). The HL60 cell line was treated with different concentrations of RJ for 24, 48, and 72 hours. The half maximum inhibitory concentration (IC50) of RJ was determined using 3-(4.5 dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide test (MTT) and the proliferation activity of HL-60 cells was evaluated. Flow cytometry analysis was performed to measure apoptosis in HL-60 cells. IC50 values for RJ were calculated as 13.98, 6.45, and 2.06 mg/mL for 24, 48, and 72 hours, respectively. Flow cytometry results also showed that RJ had apoptotic effects at the concentrations found. The results showed that RJ treatment significantly induced apoptosis and reduced the proliferation of HL-60 cells. This study shows that RJ can be a complementary treatment against HL-60 acute myeloid leukemia cells due to its anticancer and antiproliferative effects.

Anahtar Kelimeler

Royal Jelly, HL-60, Anti-cancer, Apoptosis, MTT

Etik Beyan

There is no problem in terms of research and publication ethics.

Destekleyen Kurum

No support was received from any institution for the study..

Teşekkür

We would like to thank Prof. Dr. Seyithan TAYSI, Department of Medical Biochemistry, Gaziantep University, for providing laboratory facilities and necessary materials.

Arı Sütünün HL-60 Hücreleri Üzerindeki Apoptoz Aracılı Sitotoksik Etkilerinin Araştırılması

Öz

Son yıllarda mevcut kanser ilaçlarının olumsuz yan etkilerini ve normal hücrelere olan toksisiteyi zararlarını azaltmak için kanser hücrelerinde etkili olabilecek nontoksik doğal ürünlerin yeni ajan olarak kullanılması bilim insanlarının dikkatini çekmektedir. Bazı in vitro ve in vivo çalışmalar, arı sütünün hücre proliferasyonunu inhibe ettiğini, apoptozu indüklediğini göstermiştir. Bu çalışmada, arı sütünün insan akut miyeloid lösemi hücre hattında proliferatif ve apoptotik süreçlerdeki etkilerini araştırmayı amaçladık. HL60 hücre hattı, 24, 48 ve 72 saat boyunca farklı konsantrasyonlarda arı sütü ile muamele edildi. 3-(4,5 dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür testi (MTT) kullanarak arı sütünün yarı maksimum inhibitör konsantrasyonu (IC50) belirlendi ve HL-60 hücrelerinin proliferasyon aktivitesi değerlendirildi. HL-60 hücrelerindeki apoptozu ölçmek için flow sitometresi analizi gerçekleştirildi. Yapılan hesaplamalarda arı sütü için IC50 değerleri 24, 48 ve 72 saat için sırasıyla 13,98, 6,45 ve 2,06 mg/mL bulundu. Flow sitometri sonuçlarıde arı sütünün bulunan konsantrasyonlarda apoptotik etkilere sahip olduğunu gösterdi. Sonuçlar, arı sütü tedavisinin HL-60 hücrelerinin proliferasyonunu önemli ölçüde azalttığını ve apoptozunu indüklediğini gösterdi. Bu çalışma, arı sütünün antikanser ve antiproliferatif etkilerinden dolayı HL-60 akut miyeloid lösemi hücrelerine karşı tedavide tamamlayıcı olarak kullanılabileceğini göstermektedir.

Anahtar Kelimeler

Arı sütü, HL-60, Anti-kanser, Apoptoz, MTT

Etik Beyan

Araştırma ve yayın etiği açısından herhangi bir sorun bulunmamaktadır.

Destekleyen Kurum

Çalışma için herhangi bir kurumdan destek alınmamıştır.

Teşekkür

Laboratuvar olanakları ve gerekli malzemeler konusunda vermiş olduğu desteklerden dolayı için Gaziantep Üniversitesi Tıbbi Biyokimya öğretim görevlisi Prof. Dr. Seyithan TAYSI'ya teşekkür ederiz.

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