Potential Use of Beauveria bassiana (Balsamo) Vuillemin in biological control of Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae)

Year 2025, Volume: 10 Issue: 3, 321 - 327, 30.05.2025

Nasibe Tekiner Aydın , Elif Tozlu , Göksel Tozlu

https://doi.org/10.35229/jaes.1660970

Abstract

The use of entomopathogenic fungi as biological control agents against storage pests has become an effective method to reduce postharvest losses. This study aimed to determine the insecticidal effect of Beauveria bassiana, an important entomopathogenic fungus, on Sitophilus zeamais adults, known as the most important storage pest in maize. B. bassiana (ET 10) isolate prepared in three different spore suspensions (106, 107 and 108 conidia/ml + Tween 80 (0.02%)) was applied to S. zeamais adults by the spraying method. The experiment was set up in three replicates according to the randomized plot design. For each suspension, one experimental group was set with corn grains and 10 adult S. zeamais adults and the number of dead insects was recorded every 24 hours and the data was recorded until the conclusion 264 hours. As a result of the application, mortality were observed in all suspensions from the first 24 hours, reaching 100% in the 108 conidia/ml suspension at the 144th hour. At the end of 264 hours, the mortality rates were recorded as 26.7% for 106 conidia/ml, 36.7% for 107 conidia/ml, 100% for 108 conidia/ml. The most effective dose among the application suspensions was determined to be 108 conidia/ml. It has been proven that B. bassiana ET 10 isolate can be used as a potential bioagent as an alternative in the biological control of S. zeamais and that biological control with entomopathogenic fungi can be a correct and positive strategy.

Keywords

Beauveria bassiana , Biological control , Maize weevil , Mortality rate , Zea mays L.

Sitophilus zeamais (Mots.) (Coleoptera: Curculionidae)’in biyolojik mücadelesinde Beauveria bassiana ET 10’nun kullanılma potansiyeli

Abstract

Entomopatojen fungusların depo zararlısı böceklere karşı biyolojik mücadele ajanı olarak kullanımı, hasat sonrası kayıpları etkili bir şekilde azaltan iyi bir yöntem haline gelmiştir. Bu çalışmada önemli bir entomopatojen fungus olan Beauveria bassiana (Balsamo) Vuillemin’in, mısırda en önemli depo zararlısı olarak bilinen Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae) erginleri üzerindeki insektisidal etkisinin laboratuvar şartları altında belirlenmesi amaçlanmıştır. Üç farklı süspansiyonda (106, 107 ve 108 konidi/ml+ Tween 80 (%0.02)) hazırlanmış B. bassiana (ET 10) izolatı spreyleme yöntemi ile S. zeamais erginleri üzerine uygulanmıştır. Deneme tesadüf parselleri desenine göre üç tekerrürlü olarak kurulmuştur. Her bir süspansiyona mısır taneleri ile 10 adet ergin bireyi S. zeamais konulmuş ve her 24 saatte bir ölü böcek sayıları kaydedilerek 264 saat sonuna kadar veriler kaydedilmiştir. Uygulama sonucu ilk 24 saatten itibaren ölümler bütün süspansiyonlarda gözlemlenmiş ve 144. saatte 108 konidi/ml süspansiyonda %100 olmuştur. 264 saat sonunda sırasıyla (106, 107 ve 108 konidi/ml) yüzde ölüm oranı %26.7, %36.7, %100 olarak kaydedilmiştir. Uygulama süspansiyonları arasında en etkili dozun 108 konidi/ml olduğu belirlenmiştir. ET 10 izolatının bu böceğin biyolojik mücadelesinde alternatif olarak potansiyel bir biyoajan olarak kullanılabileceğini ve entomopatojen funguslar ile biyolojik mücadelenin doğru ve olumlu bir mücadele yöntem stratejisi olabileceğini kanıtlamıştır.

Keywords

Beauveria bassiana , Biyolojik mücadele , Mısır biti , Ölüm oranı , Zea mays L.

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