Analysis of Essential Oil Composition of Endemic Marrubium parviflorum subsp. oligodon Grown in Turkey by Using SPME with GC/MS
Abstract
The aim of the study is to determine the essential oil profile and antioxidant activity of endemic Marrubium parviflorum subsp. oligodon. The essential oil contents were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) using Solid Phase Microextraction (SPME). In addition, total phenolic content and the capacity of antioxidant activity measured by DPPH• and FRAP assays were determined. Twenty-three (64.12%) essential oil components were determined. Pentadecanolide was found to be the major essential oil and palmitic acid was determined as main fatty acid in Marrubium parviflorum subsp. oligodon. Total phenolic content was found to be 39.9±0.31 mg GAE/g sample. FRAP value was found to be 48.91±0.33 µmol Fe/g sample and DPPH• scavenging activity was found to be 0.76±0.03 mg/mL. This study is the first report in which the essential oil content of Marrubium parviflorum subsp. oligodon was determined by SPME.
Keywords
Marrubium parviflorum subsp. oligodon, SPME, Lamiaceae, essential oil.
References
- Akgul, G., Ketenoglu, O., Pinar, N. M., & Kurt, L. (2008). Pollen and seed morphology of the genus Marrubium (Lamiaceae) in Turkey. Annales Botanici Fennici, 45, 1–10.
- Altundag, E., & Ozturk, M. (2011). Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Procedia Social and Behavioral Sciences, 19, 756–777.
- Bal, Y., Kaban, S., Kirimer, N., & Baser, K. H. C. (1999). Composition of the essential oil of Marrubium parviflorum Fisch. et Mey. subsp. oligodon (Boiss.) Seybold. Journal of Essential Oil Research, 11, 300-302.
- Balasundram, N., Sundram, K., & Samman, S. (2006). Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chemistry, 99, 191-203.
- Benzie, I. F. F., & Szeto, Y. T. (1999). Total antioxidant capacity of teas by the ferric reducing/antioxidant power assay. Journal of Agricultural and Food Chemistry, 47, 633–636.
- Bishop, C. D. (1995). Antiviral activity of the essential oil of Melaleuca alternifolia (Maiden and Betche) Cheel (tea tree) against tobacco mosaic virus. Journal of Essential Oil Research, 7(6), 641- 644.
- Boyle, W. (1955). Spices and essential oils as preservatives. The American Perfumer and Essential Oil Review, 66, 25-28.
- Ceylan, A. (1983). Tıbbi Bitkiler-II. Ege Üniversitesi Ziraat Fakültesi Yayını No:481, Bornova-İzmir.
- Ceylan, Ş., Yardımcı, Ş.S., Camadan, Y., Saral, Ö., & Batur, Ö. Ö. (2021). Chemical composition of essential oil by SPME and evaluation of antimicrobial, antioxidant activities of Quercus inectoria Gall. Acta Scientiarum Polonorum Hortorum Cultus, 20, 93-103.
- Ceylan, Ş., Camadan, Y., Saral, Ö., & Batur, Ö. Ö. (2022). Chemical composition, antimicrobial, and antioxidant activities of medicinal plants nutsedge and fenugreek. BioResources, 17, 4580-4594.