Primula vulgaris L.'nin Sıçanlarda Metotreksat Kaynaklı Testis Hasarına Karşı Antioksidan ve Antiapoptotik Etkileri

Year 2024, Volume: 7 Issue: 2, 154 - 169, 29.12.2024

Murat Berber , Merve Badem , Beyza Ayan , Şeyda Kanbolat , Sıla Özlem Sener , Rezzan Aliyazıcıoğlu , Engin Yenilmez , Sermet Yıldırmış , Diler Us Altay , Ufuk Özgen

https://doi.org/10.35206/jan.1528954

https://izlik.org/JA77YD97AA

Abstract

Bu çalışmanın amacı metotreksat (MTX) kaynaklı testis hasarına karşı Primula vulgaris ekstraktının antioksidan ve antiapoptotik etkilerini araştırmaktır. Bu çalışmada her grupta 8 adet sıçan olacak şekilde 4 grup oluşturulmuştur. Grup 1’deki sıçanlara 7 gün boyunca 0.8 mg/kg serum fizyolojik gavaj ile verilmiştir. Grup 2’deki sıçanlara çalışmanın ilk günü tek doz (30 mg/kg) MTX intraperitoneal olarak uygulanmıştır. Grup 3’deki sıçanlara çalışmanın ilk günü tek doz (30 mg/kg) MTX uygulanmış ve daha sonra ilk günden başlayarak 7 gün boyunca 100 mg/kg sulu ekstrakt gavaj yoluyla verilmiştir. Grup 4’deki sıçanlara 7 gün boyunca 100 mg/kg sulu ekstrakt gavaj yoluyla verilmiştir. 8. gün anestezi altında sıçanların testisleri ve epididimisleri çıkarılmış, kanları alınmıştır. Çıkarılan testisler histolojik ve biyokimyasal analizler için kullanılmıştır. Grup 2, grup 1 ile kıyaslandığında seminifer tübül çapı, epitel kalınlığı, sperm sayısı, motilite, vitalite ve Johnsen skorlama değerlerinin azaldığı; lümenine immatür hücre dökülen tübül sayısı ve apoptotik indeksin arttığı belirlenmiştir. Grup 3, grup 2’ye göre seminifer tübül çapı, epitel kalınlığı, sperm sayısı, motilite, vitalite ve Johnsen skorlama değerlerinin arttığı; lümenine immatür hücre dökülen seminifer tübül sayısı ve AI’nın azaldığı gözlenmiştir. Grup 2, grup 1 ile kıyaslandığında kan plazmasında ve testis dokusunda MDA değerinin arttığı, SOD ve CAT değerlerinin ise azaldığı bulunmuştur. Çalışma sonuçlarına göre MTX’in testiste oksidatif stres oluşturarak testise zarar verdiği, Primula vulgaris’in ise antioksidan etkileri sayesinde bu hasarı azalttığı belirlenmiştir.

Keywords

Antioksidan , Apoptozis , Primula vulgaris , Testis

Project Number

TYL-2015-5341

Antioxidant and Antiapoptotic Effects of Primula vulgaris L. Against Methotrexate-Induced Testicular Damage in Rats

https://izlik.org/JA77YD97AA

Abstract

The aim of this study was to investigate the antioxidant and antiapoptotic effects of Primula vulgaris extract against methotrexate (MTX)-induced testicular damage. In this study, 4 groups were formed with 8 rats in each group. Rats in group 1 were given 0.8 mg/kg physiological serum via gavage for 7 days. The rats in group 2 were administered a single dose (30 mg/kg) of MTX intraperitoneally on the first day of the study. The rats in group 3 were administered a single dose (30 mg/kg) of MTX on the first day of the study and then 100 mg/kg of aqueous extract via gavage for 7 days starting from the first day. The rats in group 4 were given 100 mg/kg of aqueous extract via gavage for 7 days. On the 8th day, the testicles and epididymis of the rats were removed under anesthesia and their blood was collected. The removed testicles were used for histological and biochemical analyses. When group 2 was compared with group 1, it was determined that seminiferous tubule diameter, epithelial thickness, sperm count, motility, vitality, and Johnsen scoring values decreased; tubule number that immature cells sloughed into the lumen and apoptotic index (AI) increased. In group 3, it was observed that seminiferous tubule diameter, epithelial thickness, sperm count, motility, vitality, and Johnsen scoring values increased; tubule number that immature cells sloughed into the lumen and AI decreased compared to group 2. When group 2 was compared with group 1, it was found that MDA values increased, and SOD and CAT values decreased in blood plasma and testicular tissue. According to the study results, it was determined that MTX caused damage to the testicle by creating oxidative stress, while Primula vulgaris reduced this damage thanks to its antioxidant effects.

Keywords

Antioxidant , Apoptosis , Primula vulgaris , Testes

Supporting Institution

Office of Scientific Research Projects of Karadeniz Technical University

Project Number

TYL-2015-5341

Thanks

This work was supported by Office of Scientific Research Projects of Karadeniz Technical University (Project number TYL-2015-5341).

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