Cell-based Cytotoxicity Methods

Albandri Alfraidi; Theara Cendi Fagundes

EN

Cell-based Cytotoxicity Methods

Abstract

Cell-based cytotoxicity methods are an essential part of cancer research and drug discovery. Cancer is characterized by increasing in the cells proliferation and decreasing in cell death. Likewise, drugs interfere with many cellular functions that involve different cell death pathways. These changes in the physiology of the cells can be qualified with several available methods. Many assays rely on numerous cell functions from metabolic activity and cellular Adenosine 5′-triphosphate (ATP), membrane integrity, DNA cleavage, caspase activity, protein content and mitochondria changes. Some assays are considered suitable for high throughput screening due to the rapid evaluation of many samples in a cost-effective way, in addition to low sample and reagent consumption. Selecting the right methods is critical to generating meaningful results. There are many molecular signaling pathways involved in cell deaths and every assay gives specific insight into the process of apoptosis in the cells. This helps in the data interpretation for a better understanding of specific cell death mechanisms for in vitro analysis. This review is given an overview of the most commonly used methods to assess cell death.

Keywords

cytotoxic assays,in vitro,apoptosis

References

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Details

Primary Language

English

Subjects

-

Journal Section

Review

Authors

Albandri Alfraidi ^*
Departamento de oncologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais
Brazil

Theara Cendi Fagundes This is me
Brazil

Publication Date

April 1, 2018

Submission Date

January 1, 2018

Acceptance Date

January 18, 2018

Published in Issue

Year 2018 Volume: 1 Number: 1

IZ

https://izlik.org/JA78NS54RD

APA

Alfraidi, A., & Cendi Fagundes, T. (2018). Cell-based Cytotoxicity Methods. Journal of Apitherapy and Nature, 1(1), 3-12. https://izlik.org/JA78NS54RD

AMA

1.Alfraidi A, Cendi Fagundes T. Cell-based Cytotoxicity Methods. J.Apit.Nat. 2018;1(1):3-12. https://izlik.org/JA78NS54RD

Chicago

Alfraidi, Albandri, and Theara Cendi Fagundes. 2018. “Cell-Based Cytotoxicity Methods”. Journal of Apitherapy and Nature 1 (1): 3-12. https://izlik.org/JA78NS54RD.

EndNote

Alfraidi A, Cendi Fagundes T (April 1, 2018) Cell-based Cytotoxicity Methods. Journal of Apitherapy and Nature 1 1 3–12.

IEEE

[1]A. Alfraidi and T. Cendi Fagundes, “Cell-based Cytotoxicity Methods”, J.Apit.Nat., vol. 1, no. 1, pp. 3–12, Apr. 2018, [Online]. Available: https://izlik.org/JA78NS54RD

ISNAD

Alfraidi, Albandri - Cendi Fagundes, Theara. “Cell-Based Cytotoxicity Methods”. Journal of Apitherapy and Nature 1/1 (April 1, 2018): 3-12. https://izlik.org/JA78NS54RD.

JAMA

1.Alfraidi A, Cendi Fagundes T. Cell-based Cytotoxicity Methods. J.Apit.Nat. 2018;1:3–12.

MLA

Alfraidi, Albandri, and Theara Cendi Fagundes. “Cell-Based Cytotoxicity Methods”. Journal of Apitherapy and Nature, vol. 1, no. 1, Apr. 2018, pp. 3-12, https://izlik.org/JA78NS54RD.

Vancouver

1.Albandri Alfraidi, Theara Cendi Fagundes. Cell-based Cytotoxicity Methods. J.Apit.Nat. [Internet]. 2018 Apr. 1;1(1):3-12. Available from: https://izlik.org/JA78NS54RD

e-ISSN: 2667-4734 Publication Model: Continuous Publication Citation Indexes: TR Dizin

Cell-based Cytotoxicity Methods

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Cell-based Cytotoxicity Methods

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