Plant Origin Authentication of Sonoran Propolis and its Antiproliferative Effect on Cancer Cells: A Bioactive Poplar Type Propolis from Semi-arid Zones
Abstract
The main chemical
composition of Sonoran propolis (SP), as well as its antiproliferative activity
on cancer cells via apoptosis induction has been reported1.
Additionally, the chemical constitution of SP remained qualitatively similar
throughout the year, whereas the antiproliferative effect exhibited significant
differences amongst seasonal samples2. The goal of this study was to
authenticate the botanical
source of SP bioactivity by using an approach based
on a chemical comparative analysis, antiproliferative activity and cell cycle
progression analysis on cancer cell lines. The polyphenolic profile of SP
throughout the year resulted to be qualitatively similar to that of Populus
fremontii resins (PFR). However, the antiproliferative activity of PFR did
not consistently match that exhibited by SP. In addition, SP induced evident
morphological modifications (elongation) on treated cells, different to those
induced by PFR. Most of cancer cells treated with SP were arrested in G2/M
checkpoint (M12.C3.F6: 94.8±1.7 %, and HeLa cells: 70.8±5.7 %), similarly as
colchicine did (control drug; 2 µM; 94.8±2.3 % and 72.7±6.4 %, respectively).
In contrast, PFR treatment increased cell population at G0/G1
on both M12.C3.F6 (56.9±5.0 %) and HeLa cells (70.3±0.3 %), in comparison with
dissolvent control. Interestingly, Ambrosia confertiflora resins induced
morphological elongation and cell cycle arrest at G2/M on both
M12.C3.F6 and HeLa cells, similarly as SP did. These results suggest that P.
fremontii is the main plant origin of SP, nevertheless, A. confertiflora
resins participate as a complementary source that enhances its bioactivity.
Therefore, SP is a poplar-type propolis from subtropical semi-arid zones.
Keywords
References
- 1. Alday E, Valencia D, Carreсo AL, Picerno P, Piccinelli AL, Rastrelli L, Robles-Zepeda R, Hernandez J, Velazquez C (2015) Apoptotic induction by pinobanksin and some of its ester derivatives from Sonoran propolis in a B-cell lymphoma cell line. Chemico-Biological Interactions 242: 35–44. 2.Valencia D, Alday E, Robles-Zepeda R, Garibay-Escobar A, Galvez-Ruiz JC, Salas-Reyes M, Jimйnez-Estrada M, Velazquez-Contreras E, Hernandez J, Velazquez C (2012). Seasonal effect on chemical composition and biological activities of Sonoran propolis. Food Chemistry 131: 645–651.