Formulations Containing Propolis of Apis mellifera with Antibacterial and Antitumoral Activity
Abstract
Propolis, a resin produced by A. mellifera bees, presents several compounds of pharmaceutical
interest and, further studies to demonstrate their pharmacological actions is
sought. Capsules containing green propolis (BRPX) extracted in flax oil (1 and 2) polyethylene glycol (PEG) (3
and 4) were analyzed and analyzed
according to IN 31. The following parameters were evaluated: total
phenols and flavonoids, quantification of Artepillin-C
(HPLC)
confirmed by UHPLC-MS, antitumor activity in MCF-7 (breast code 0162) and
B16F10 (melanoma code 0046) and antimicrobial activity (E.coli, C. albicans and S .aureus). The flavonoid content (%) of
each capsule was 1: 1.135 ± 0.009, 2: 0.521 ± 0.039, 3: 0.758 ± 0.020, 4:
1.232 ± 0.09. The phenol content (%) of 1:
2,509 ± 0,024, 2: 2,280 ± 0,075, 3: 1,039 ± 0,029, 4: 3,426 ± 0,074. The content of Artepillin-C (mg / capsule) of 1: 1.39 ± 0.12, 2: 1.07 ± 0.05, 3: 0.66
± 0.01, 4: 0.39 ± 0, 01. The IC50
(μg / mL) for MCF-7 was: 1: 100.49 ±
6.41, 2: 463.65 ± 0.71, 3: 209.03 ± 35.99, 4: 238.84 ± 4 , 16 and for B16F10, 1: 277.53 ± 7.37, 2:
357.48 ± 10.15, 3: 376.15 ± 5.92, 4: 286.87 ± 10.79. Inhibition halos
ranged from 10 to 14 mm (E.coli), 8
to 10 mm (C. albicans) and 8 to 9 mm
(S.aureus). This study demonstrates
the importance of quality control for reliable formulations based on propolis.
Keywords
References
- 1. BRASIL (2001). MINISTRY OF AGRICULTURE. Normative Instruction nº 3 - ANNEX VII - Technical regulation for determination of identity and quality of propolis. Diário Oficial da República Federativa do Brasil.