Antituberculosis Activity of Propolis
Abstract
Tuberculosis an infectious disease caused by Mycobacterium tuberculosis is one of the
leading causes of human morbidity and mortality. In 2013 the WHO reported one
and half million of deaths and nine millions new cases of active tuberculosis
caused by TB1.The increase in the incidence of clinical tuberculosis
is associated with increasing reports of new cases of multi drug resistant
(MDR-TB) and extensively multidrug resistant (XDR-TB) strains1.
Propolis (bee glue) is well known for its
antibacterial and antifungal properties.More than 30 ethanol-water extracts
obtained from different propolis samples were tested for antituberculosis
activity.
Minimal Inhibitory Concentrations (MIC) values for the
EO were established with 96-well micro plate method with AlamarBlue
(Invitrogen). Reference strain of Mycobacterium tuberculosis H37Ra
inoculum in Middlebrook 7H9 broth (Difco) was 5x105cfu/ml per well,
accordingly to CLSI standards. Serial twofold dilutions of propolis extracts
ranged from 64 to 8 µg/ml. As the internal control of the method serial twofold
dilutions of four first line antibiotics dedicated to the tuberculosis
treatment: isoniazid (INH), rifampicin (RMP), ethambutol (EMB) and streptomicin
(SM) were used2,3.
Keywords
References
- 1. WHO (2014). Global tuberculosis report (www.who.int/tdr/news/2014/global-TB-report/en/).World Health Organization (2010). Multidrug and extensively drug resistant TB (M/ XDR-TB) Global report on surveillance and response. Geneva: World Health
- 2 Palomino, J.C., Martin, A., Camacho, A., Guerra, H., Swings, J. ,Portaels, F.(2002). Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis. Antimicrobial Agents and Chemotheraphy, 46, 2720-2722
- 3 Wayene, P.A. (2011). Susceptibility Testing of Mycobacteria, Nocarediae, and Other Aerobic Acinomycetes; Approved Standard-Second Edition. CLSI document M24-A2. USA. Clinical and Laboratory Standards Institute.