Isolation of Lignin-Degrading Bacteria from Different Sources and Testing of Their Ligninolytic Activities

Year 2019, , 30 - 45, 23.04.2020

Ayşegül Özer Erva Rakıcı Kadriye Inan Bektas Sabriye Çanakçı , Ali Osman Belduz

https://doi.org/10.35206/jan.554729

Cited By: 3

Abstract

Nine
lignin-degrading bacteria were isolated from petroleum-contaminated soil and animal
manure samples and characterized by 16S rRNA sequence analysis. Three isolates
were identified as Enterobacter cancerogenus, two as Enterobacter
ludwigii, one as Citrobacter sedlakii,
one as Citrobacter farmeri, one as Klebsiella pneumoniae, and one as Citrobacter murliniae.
These  bacteria used ligno sulphate as
the sole carbon source but  did not
utilize kraft lignin (KL) as the sole source of carbon and energy. For this reason, basic nutrients, such as 1.0% glucose
(w/v) and 0.5% peptone (w/v), were used as additional carbon and nitrogen
sources to stimulate bacterial growth for KL decolorization.
Under these conditions, the isolates L1, L2, L3, L4, PT21, PT22, PT41, G1, and
C1 degraded kraft lignin by 37 %, 14 %, 20%, 43%, 48%, 51%, 28%, 60%, and %99,
respectively. The decolorization of Remazol Brilliant Blue R (RBBR) by the
isolates was analyzed. The isolates were decolorized at 20-90 % of RBBR,
respectively. 

Keywords

Lignin, Bacteria, 16S rRNA

References

• Allocati, N., Federici, L., Masulli, M. &Di IIio, C. (2009) Glutathione transferases in bacteria. The FEBS Journal, 276(1), 58-75.
• Bal, A.S., Betts, W.B. & McCarthy, A.G. (1989) Degradation of lignin-related compounds by Actinomycetes. Appl. Environ. Microbiol., 55, 1642-1646.
• Bandounas, L., Wierckx, N., Winde, J. & Ruijssenaars, H.J. (2011) Isolation and characterization of novel bacterial strains exhibiting ligninolytic potential. BMC Biotechnology, 11(94).
• Beffa, T., Blanc, M., Lyon, P.F., Vogt, G., Marchiani, M., Fischer, J.L. & Aragno, M. (1996) Isolation of Thermus Strains from hot composts. Appl. Environ. Microbiol., 62, 1723-1727.
• Bholay, A.D., Borkhataria Bhavna, V., Jadhav Priyanka, U., Palekar Kayeri, S., Dhalkari Mayuri, V. & Nlawade, P.M. (2012) Bacterial lignin peroxidase: A tool for bioleaching and biodegradation of industrial effluents. Universal journal of Environmental Research and Technology, 2(1), 58-64.
• Canas, A.I., Alcalde, M., Plou, F., Martinez, F.J., Martinez, A.T. & Camerero, S. (2007) Transformation of polycyclic aromatic hydrocarbons by laccase is strongly enhanced by phenolic compounds present in soil. Environmental Science & Technology, 41(8), 2964-2971.
• Chandra, R., Raj, A., Purohit, H.J. & Kapley, A. (2007) Characterization and optimization of three potential aerobic bacterial strains for kraft lignin degradation from pulp paper waste. Chemosphere, 67 (4), 839–846.
• Deschamps, A.M., Mahoudeau, G. & Lebeault, J.M. (1980) Fast degradation of Kraft lignin by bacteria. Appl. Microbiol. Biotechnol., 9, 45-51.
• EL-Hanafy, A.A., Abd-Elsalam, H.E. & Hafez, E.E. (2008). Molecular characterization of two native Egyptian ligninolytic bacterial strains. J. Appl. Sci. Res., 4(10), 1291-1296.Falcon, M.A., Rodriguez, A., Carnicero, A., Regalado, V., Perastelo, F., Milstein, O. & Fuente, G. (1995) Isolation of microorganisms with lignin transformation potential from soil of Tenerife Island. Soil Biol. Biochem., 27, 121–6.
• Franc, M., Hullo, O., Moszer, I., Danchin, A. & Martin-Verstraete, I. (2001) CotA of Bacillus subtilis is a copper-dependent laccase. Journal of Bacteriology, 183(18), 5426-5430.
• Gottschalk, M.F., Macedo, M.B. & Bon, P.S. (1999) Lignin peroxidase production by Streptomyces viridosporus T7A use of corn oil as a carbon source. Applied Biochemistry and Biotechnology, 77(79). 771-778.
• Hatakka, A. (1994) Lignin-modifying enzyme from selected white-rot fungi: production and role in lignin degradation. FEMS Microbiology Review, 13, 125–135.
• Hernandez, M., Hernandez-Coronado, M.J., Montiel, M.D., Rodriquez, J. & Arias, M.E. (2001) Analysis of alkali-lignin in a paper mill effluent decolorised with two Streptomyces strains by gas chromatography-mass spectrometry after cupric oxide degradation. J. Chromatogr., 919, 389–394.
• Kirk, T.K. & Farrell, R.L. (1987) Enzymatic “combustion”: The microbial degradation of lignin. Annual Review of Microbiology, 41(1), 465-501.
• Lopez, M.J., Guisado, G., Vargas-Garcia, M.C., Suarez-Estella, F. & Moreno, J. (2006) Decolorization of industrial dyes by ligninolytic microorganisms isolated from composting environment. Enzyme and Microbial Technology, 40, 42-45.
• Maciel, M.J.M., Silva, A.C. & Ribeiro, H.C.T. (2010) Industrial and biotechnological applications of ligninolytic enzymes of the basidiomycota: A review. Enviromental Biotechnology, 13(6), 14-15.
• Masai, E., Ichhimura, A., Sato, Y., Miyauchi, K., Katayama, Y. & Fukuda, M., 2003. Roles of the enantioselective glutathione S-transferase in cleavage of beta-aryl ether. Journal of Bacteriology, 185(6), 1768-1775.
• McKay, G. (1979) Waste colour removal from textile effluents. Am. Dyes. Rep., 68, 29–36.
• McMullan, G., Meehan, C., Conneely, A., Kirby, N., Robinson, T., Nigam, P., Banat, I.M., … Smyth, W.F. (2001) Microbial decolourisation and degradation of textile dyes. Appl. Microbiol. Biotechnol., 56, 81–87.
• Morii, H., Nakamiya, K. & Kinoshita. S. (1995) Isolation of lignin decolouring bacterium. J. Ferment. Bioeng., 80, 296-299.
• Nascimento, H.J. & Silva, E.J.G. (2008) Purification of lignin peroxidase isoforms from Streptomyces viridosporus T7A by hydrophobic based chromatographies. World J. Microbiol. Biotechnol., 24, 1973-1975.
• Nishimura, M., Ooi, O. & Davies, J. (2006) Isolation and characterization of Streptomyces sp. NL15-2K capable of degrading lignin-related aromatic compounds. J. Biosci. Bioeng., 102, 124-127.
• Odier, E., Janin, G. & Monties, B. (1981) Poplar lignin decomposition by gram-negative aerobic bacteria. Appl. Environ. Microbiol., 41, 337-341.
• Oliveira, P., Duarte, M.C.T., Ponezi, A.N. & Durrant, L.R. (2009) Purification and partial characterization of manganese peroxidase from Bacillus pumilus and Paenibacillus sp. Brazilian Journal of Microbiology, 40, 818-826.
• Ollikka, P., Alhonmaki, K., Leppanen, V.M., Glumoff, T., Raijola, T. & Suominen, I. (1993) Decolorization of azo, triphenyl methane, heterocyclic and polymeric dyes by lignin peroxidase isoenzymes from Phanerochaete chrysosporium. Appl. Environ. Microbiol., 59(12), 4010-4016.
• Özsoy, D.H., Ünayar, A. & Mazmancı, M.A. (2005) Decolourisation of reactive textile dyes Drimarene Blue X3LR and Remazol Brillant Blue R by Funali trogii ATCC200800. Biodegradation, 16, 195-204.Pasti, M.B. & Crawford, D.L. (1991) Relationships between the abilities of streptomycetes to decolorize three anthron-type dyes and to degrade lignocellulose. Can. J. Microbiol., 37, 902–907.
• Perestelo, F., Falcon, M.A., Perez, M.L., Roig, E.C. & Martin, G.F. (1989) Bioalteration of kraft pine lignin by bacillus megaterium ısolated fromcompost piles. J. Fermen. Bioeng., 68(2), 151-153.
• Pometto, A.L. & Crawford, D.L. (1986) Effect of pH on lignin and cellulose degradation by Streptomyces viridosporus. Appl. Environ. Microbiol., 52, 246-250.
• Raj, A., Chandra, R., Reddy, M.M.K., Purohit, H.J. & Kapley, A. (2007) Biodegradation of kraft lignin by newly isolated bacterial strain, Aneurinibacillus aneurinilyticus from the sludge of a pulp paper mill. World J. Microbiol. Biotechnol., 23, 793-799.
• Rayner, A.D.M. & Boddy, L. (1988) Fungal decomposition of wood. Its biology and ecology. Wiley, New York.
• Reiss, R., Ihssen, J. & Thöny-Meyer, L. (2011) Bacillus pumilus laccase: a heat stable enzyme with a wide substrate spectrum. BMC Biotechnology, 11(9).
• Rodriguez, E., Pickard, M.A. & Vazquez-Duhalt, R. (1999) Industrial dye decolorization by laccases from ligninolytic fungi. Current Microbiol., 38, 27-32.
• Ruiz-Dueñas, F.J. & Martínez, A.T. (2009) Microbial degradation of lignin: how a bulky recalcitrant polymer is efficiently recycled in nature and how we can take advantage of this. Microbial Biotechnology, 2(2). 164-177.
• Somogyi, M. (1952) Notes on sugar determination. J. Biol. Chem., 195, 19-23.
• Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol., 24, 1596-1599.Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmouqin, F. & Higgins, D.G. (1997) The ClustalX window interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res., 24, 4876-4882.
• Tien, M. & Kirk, T.K. (1983) Lignin-degrading enzymes from himenomycete Phanerochaete chrysosporium. Burds Science., 221, 661–663.
• Trojanowski, J., Haider, K. & Sundman, V. (1977) Decomposition of 14C-labelled lignin and phenols by a Nocardia sp. Arch. Microbiol., 114, 149–153.