Royal Jelly and Its SDS-PAGE Electrophoresis Profiles
Abstract
Royal jelly (RJ) is an important functional food, is used for many
purposes. New methods are needed for the practical characterization of royal
jelly. In this preliminary study, SDS-PAGE electrophoretic profiles of royal
jelly proteins were investigated. Raw royal jelly samples were analyzed by
classical SDS-PAGE electrophoresis using 5% and 10% gels. The major protein
spot were obtained at the samples collected same regions. Compared the proteins
with known marker proteins they were between 50 and 80 kDa. Classical SDS-PAGE
electrophoresis alone is not sufficient to determine royal jelly proteins, but
can be used to basic characterization of royal jelly. Furthers electrophoretic
techniques are needed for a better separation.
Keywords
References
- Albert, Š., & Klaudiny, J. (2004). The MRJP/YELLOW protein family of Apismellifera: Identification of new members in the EST library. Journal of Insect Physiology, 50(1), 51-59.
- Albert, Š., Klaudiny, J., & Šimúth, J. (1999). Molecular characterization of MRJP3, highly polymorphic protein of honeybee (Apis mellifera) royal jelly. Insect Biochemistry and Molecular Biology, 29(5), 427-434.
- Fujiwara, S., Imai, J., Fujiwara, M., Yaeshima, T., Kawashima, T., & Kobayashi, K. (1990). A potent antibacterial protein in royal jelly. Purification and determination of the primary structure of royalisin. Journal of Biological Chemistry, 265(19), 11333-11337.
- Guo, H., Kouzuma, Y., & Yonekura, M. (2009). Structures and properties of antioxidative peptides derived from royal jelly protein. Food Chemistry, 113(1), 238-245.
- Kolayli, S., Sahin, H., Can, Z., Yildiz, O., Malkoc, M., & Asadov, A. (2016). A member of complementary medicinal food: anatolian royal jellies, their chemical compositions, and antioxidant properties. Journal of Evidence-Based Complementary & Alternative Medicine, 21(4), NP43-NP48.
- Pasupuleti, V. R., Sammugam, L., Ramesh, N., & Gan, S. H. (2017). Honey, propolis, and royal jelly: a comprehensive review of their biological actions and health benefits. Oxidative medicine and cellular longevity, 2017.
- Ramadana, M. F., & Al-Ghamdi, A. (2012). Bioactive compounds and health promoting properties of royal jelly: a review, Journal of Functional Foods, 4, 39 –52.
- Sabatini, A. G., Marcazzan, G. L., Caboni, M. F., Bogdanov, S., & Almeida-Muradian, L. B. D. (2009). Quality and standardisation of royal jelly. Journal of ApiProduct and ApiMedical Science, 1(1), 1-6.
- Sano, O., Kunikata, T., Kohno, K., Iwaki, K., Ikeda, M., & Kurimoto, M. (2004). Characterization of royal jelly proteins in both Africanized and European honeybees (Apis mellifera) by two-dimensional gel electrophoresis. Journal of Agricultural and Food Chemistry, 52(1), 15-20.
- Scarselli, R., Donadio, E., Giuffrida, M. G., Fortunato, D., Conti, A., Balestreri, E., ... & Felicioli, A. (2005). Towards royal jelly proteome. Proteomics, 5(3), 769-776.