Türkiye’nin Batı Karadeniz Bölgesinde Düzce İli Yığılca İlçesinde Üretilen Propolisin Fenolik İçeriğinin Araştırılması

Year 2018, Volume: 1 Issue: 2, 20 - 28, 29.10.2018

Pınar Göç Rasgele , Meral Kekecoglu

Abstract

Son yıllarda tüketici bilincinin artmasıyla birlikte, gıda, ilaç, kozmetik gibi pek çok sektörde doğal ürünlerin kullanımı önem kazanmıştır ve Propolis bu doğal ürünlerden biridir. Bu çalışmanın amacı, tıbbi ve biyolojik yararlığı nedeniyle, Türkiye'nin batı Karadeniz Bölgesinde yer alan Düzce İli Yığılca ilçesinde üretilen propolis örneklerinin kimyasal bileşimlerini araştırmaktır. Düzce Üniversitesi Arıcılık Araştırma, Geliştirme ve Uygulama Merkezi'ndeki (DAGEM) ve farklı arılıklardaki kovanlara yerleştirilen plastik propolis tuzaklarından ham propolisler toplandı. Etanolik propolis ektraktı hazırlandı ve diyot dizisi detektörü (HPLC-DAD) ile yüksek performanslı sıvı kromatografisi kullanılarak analiz edildi. Sonuçlar göstermiştir ki; Biochanin, Gallik Asit, CAPE, Pinostrobin ve Pinobankstin dominant olarak bulunmaktadır ve bu bileşenler flavonoid grubuna aittir. Tüm örneklerin farklı seviyelerde farklı biyoaktif bileşimler içerdiği gözlenmiştir. Aynı bölgeden alınan örneklerde bile kimyasal içeriğin farklı olduğu gözlenmiştir. Sonuç olarak, propolisin kimyasal içeriği flora, coğrafi konum, bal arısı alt türleri, çözücü gibi birçok faktöre bağlı olmasına rağmen, aynı bölgeden elde edilen propolis örneklerinin de içeriği ve özellikleri farklı olabilir. Bu nedenle arıcılıkta propolis standardizasyonu için kimyasal bileşiğin anlaşılması önemlidir.

Keywords

Propolisin Biyoaktif Bileşenleri, HPLC DAD

Investigation of Phenolic Content of Propolis Produced in Yigilca District of Duzce Province in Western Black Sea Region of Turkey

Abstract

With the increase of consumer consciousness in recent years, the use of natural products has gained importance in many sectors such as food, medicine, cosmetics and Propolis is one of these natural products. The purpose of this study was to investigate the chemical compositions of propolis samples from the Yigilca town of Duzce province of in the western Black Sea Region of Turkey due to its medical and biological availability. Raw propolis collected from plastic propolis traps put into top of the hive from different apiaries and Duzce University Beekeeping Research, Development and Application Center (DAGEM). Ethanolic propolis extract was prepared and analysis was performed using a high-performance liquid chromatography with diode-array detector (HPLC-DAD). The results demonstrated that Biochanin, Gallic Acid, CAPE, Pinostrobin and Pinobankstin were seen dominantly and they belong to the flavonoid group. It has been observed that all of the samples contained different bioactive compositions at different levels. It was observed that the chemical contents were different even in the samples obtained from the same region. In conclusion, the contents and properties of the propolis samples obtained from the same region may be different although the chemical content of propolis are largely dependent on many factors such as flora, geographical location, honeybee subspecies, solvent. So it is important to understand chemical compound for the propolis standardization in beekeeping.

Keywords

Bioactive Compounds of Propolis, HPLC DAD

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