GC-MS and UPLCA-PDA-TOF Profile of Polish and Eurasian Propolis

Year 2018, Volume: 1 Issue: 3 - 2nd Propolis Conference Special Issue, 78 - 78, 08.12.2018

Jarosław Wıdelskı Piotr Okıńczyc Antoni Szumny Jakub Szperlık Anna Kulma Zbigniew Sroka Krystyna Skalıcka-woźnıak Tomasz Mroczek

Abstract

Propolis, is
natural resinous substance used in beehive as a protective barrier against
bee’s enemies among them bacteria and fungi. For this properties were used in
folk medicine all over the world since centuries for treatment of different
types of wounds, burns and many others applications.

The chemical
composition of propolis is strongly connected with type of plant material
available to the bees in different regions and climate.

Chemical profile
of propolis sample determines all biological activity of this complicated
mixture and it is god indicator of its origin.

We analyzed chemical composition of propolis samples
from Poland and Eurasia (Ukraine and Russia) by two complementary techniques.
Dichloromethane extracts, rich in apolar components, were investigated by
GC-MS. More polar substances, which was presented in 70 ethanol in water
extracts, were analyzed by UPLCA-PDA-TOF. In a results, main components of
GC-MS analysed was wax alcohols and acids, some flavonoid aglicones (chrysin,
pinocembrin and tectochrysin) and trace of essential oils substances.
UPLC-PDA-TOF allowed to tracking free phenolic acid (mainly p-comuric, caffeic
and ferrulic acids), their monoesters and glycerides and flavonoids. According
to samples composition, most of them exhibited black poplar, aspen or mixed
origin.

Keywords

Propolis, GC-MS, UPLCA-PDA-TOF

References

• .