Peganum harmala ve Achillea millefolium Uçucu Yağlarının ve Kombinasyonlarının Leishmania infantum promastigotes'e Karşı Antileishmanial Aktivitelerinin Değerlendirilmesi

Year 2022, Volume: 5 Issue: 2, 93 - 102, 31.12.2022

Fatemeh Ayrom Elsever Asadov Anita Dadashkhanı Sefiqe Suleymanova

https://doi.org/10.35206/jan.1174836

Abstract

Tıbbi bitkiler ve türevleri paraziter hastalıkların tedavisinde güvenli ajanlar olarak kullanılmaktadır. Bu ön çalışma, Peganum harmala uçucu yağı (PHEO), Achillea millefolium uçucu yağı (AMEO) ve bunların kombinasyonlarının Leishmania infantum (L. infantum) promastigotlarına karşı antileishmanial aktivitelerini araştırmaktadır. Standart bir L. infantum promastigot suşu, 96 kuyulu bir Novy-MacNeal-Nicolle (NNN) kültür ortamında yetiştirildi ve 10, 100, 500 ve 1000 mg/mL konsantrasyonlarda ve 24 saat, 48 saat ve 72 saat aralık sürelerinde glucantime, PHEO, AMEO, ikisinin eşit oranında ve %80 PHEO+%20 AMEO'nun antileishmanial aktiviteleri araştırıldı. Sonuçlar, en yüksek inhibisyonun %50 PHEO + AMEO'da gözlemlendiğini ve en düşük inhibisyonun kontrol grubunda görüldüğünü gösterdi. Artan zaman ve artan konsantrasyon, verimliliklerini önemli ölçüde artırdı. Analizler, zaman ve ajanlar arasında önemli bir etkileşim olduğunu gösterdi [F (10, 360)=7.84, P=0.000]. Ajanlar, artan süre ile daha iyi etkiler gösterdi. Özetle, PHEO ve AMEO'nun eşit kombinasyonu, antileishmanial güvenli bir yapı olarak potansiyelini göstermiştir ve gelecekteki çalışmalar için dikkate alınmalıdır.

Keywords

Achillea millefolium, Antileishmanial aktivite, Peganum harmala, Promastigot

Evaluation of Antileishmanial Activities of a Peganum Harmala and Achillea Millefolium Essential oils and their combinations against Leishmania Infantum Promastigotes

Abstract

Medicinal plants and their derivations are used as safe agents for the treatment of parasitic diseases. This preliminary study investigates antileishmanial activities of Peganum harmala essential oil (PHEO), Achillea millefolium essential oils (AMEO) and their combinations against Leishmania infantum promastigotes. A standard strain of L. infantum promastigote was cultured in a 96-well Novy-MacNeal-Nicolle media culture and antileishmanial activities of glucantime, PHEO, AMEO, an equal ratio of both and 80% PHEO+20%AMEO were investigated in concentrations of 10, 100, 500 and 1000 mg/mL and interval times of 24h, 48h and 72h. The results showed that greatest inhibition was observed in 50PHEO+ AMEO and lowest inhibition was seen in control group. The increased time and increased concentration significantly increased their efficiencies. The analyses showed a significant interaction between time and agents [F (10, 360)=7.84, P=0.000]. The agents showed better effects with increased time. In sum, an equal combination of PHEO and AMEO showed its potential as an antileishmanial safe structure and must be considered for future studies.

Keywords

Achillea millefolium, Antileishmanial activity, Peganum harmala, Promastigote, Antileishmanial aktivite, Promastigot

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