Analysis of Essential Oil Composition of Endemic Marrubium parviflorum subsp. oligodon Grown in Turkey by Using SPME with GC/MS

Year 2023, Volume: 6 Issue: 1, 1 - 10, 26.06.2023

Özlem Saral , Mustafa Karaköse

https://doi.org/10.35206/jan.1213617

Abstract

The aim of the study is to determine the essential oil profile and antioxidant activity of endemic Marrubium parviflorum subsp. oligodon. The essential oil contents were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) using Solid Phase Microextraction (SPME). In addition, total phenolic content and the capacity of antioxidant activity measured by DPPH• and FRAP assays were determined. Twenty-three (64.12%) essential oil components were determined. Pentadecanolide was found to be the major essential oil and palmitic acid was determined as main fatty acid in Marrubium parviflorum subsp. oligodon. Total phenolic content was found to be 39.9±0.31 mg GAE/g sample. FRAP value was found to be 48.91±0.33 µmol Fe/g sample and DPPH• scavenging activity was found to be 0.76±0.03 mg/mL. This study is the first report in which the essential oil content of Marrubium parviflorum subsp. oligodon was determined by SPME.

Keywords

Marrubium parviflorum subsp. oligodon, SPME, Lamiaceae, essential oil.

Türkiye'de Yetiştirilen Endemik Marrubium parviflorum subsp. oligodon'un Uçucu Yağ Kompozisyonunun SPME Kullanılarak GC/MS ile Analizi

Abstract

Bu çalışmanın amacı, endemik Marrubium parviflorum subsp. oligodon'un uçucu yağ profilini ve antioksidan aktivitesini belirlemektir. Esansiyel yağ içerikleri, Katı Faz Mikro Ekstraksiyon (SPME) kullanılarak Gaz Kromatografisi-Kütle Spektrometresi (GC-MS) ile analiz edildi. Ek olarak, DPPH• ve FRAP testleri ile ölçülen antioksidan aktivite kapasitesi ve toplam fenolik madde miktarı belirlendi. Toplam 23 esansiyel yağ bileşeni (%64,12) belirlendi. Marrubium parviflorum subsp. oligodon’un toplam fenolik madde miktarı 39.9±0.31 mg GAE/g numune olarak bulundu. FRAP değeri 48.91±0.33 µmol Fe/g numune ve DPPH• süpürme aktivitesi 0.76±0.03 mg/mL olarak tespit edildi. Bu çalışma Marrubium parviflorum subsp. oligodon'un uçucu yağ içeriğinin SPME ile belirlendiği ilk rapordur.

Keywords

Marrubium parviflorum subsp. oligodon, SPME, Lamiaceae, uçucu yağ

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